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Biomarkers of waterborne copper exposure in the guppy Poecilia vivipara acclimated to salt water.
Aquat Toxicol. 2013 Aug 15; 138-139:60-9.AT

Abstract

The responses of a large suite of biochemical and genetic parameters were evaluated in tissues (liver, gills, muscle and erythrocytes) of the estuarine guppy Poecilia vivipara exposed to waterborne copper in salt water (salinity 24 ppt). Activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase, and glutathione S-transferase), metallothionein-like protein concentration, reactive oxygen species (ROS) content, antioxidant capacity against peroxyl radicals (ACAP), and lipid peroxidation (LPO) were evaluated in liver, gills, and muscle. Comet assay score and nuclear abnormalities and micronucleated cell frequency were analyzed in peripheral erythrocytes. The responses of these parameters were evaluated in fish exposed (96 h) to environmentally relevant copper concentrations (5, 9 and 20 μg L⁻¹). In control and copper-exposed fish, no mortality was observed over the experimental period. Almost all biochemical and genetic parameters proved to be affected by waterborne copper exposure. However, the response of catalase activity in liver, ROS, ACAP and LPO in muscle, gills and liver, and DNA damages in erythrocytes clearly showed to be dependent on copper concentration in salt water. Therefore, the use of these parameters could be of relevance in the scope of biomonitoring programs in salt water environments contaminated with copper.

Authors+Show Affiliations

Universidade Federal do Rio Grande, Programa de Pós-Graduação em Oceanografia Biológica, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23721848

Citation

Machado, Anderson Abel de Souza, et al. "Biomarkers of Waterborne Copper Exposure in the Guppy Poecilia Vivipara Acclimated to Salt Water." Aquatic Toxicology (Amsterdam, Netherlands), vol. 138-139, 2013, pp. 60-9.
Machado AA, Hoff ML, Klein RD, et al. Biomarkers of waterborne copper exposure in the guppy Poecilia vivipara acclimated to salt water. Aquat Toxicol. 2013;138-139:60-9.
Machado, A. A., Hoff, M. L., Klein, R. D., Cardozo, J. G., Giacomin, M. M., Pinho, G. L., & Bianchini, A. (2013). Biomarkers of waterborne copper exposure in the guppy Poecilia vivipara acclimated to salt water. Aquatic Toxicology (Amsterdam, Netherlands), 138-139, 60-9. https://doi.org/10.1016/j.aquatox.2013.04.009
Machado AA, et al. Biomarkers of Waterborne Copper Exposure in the Guppy Poecilia Vivipara Acclimated to Salt Water. Aquat Toxicol. 2013 Aug 15;138-139:60-9. PubMed PMID: 23721848.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biomarkers of waterborne copper exposure in the guppy Poecilia vivipara acclimated to salt water. AU - Machado,Anderson Abel de Souza, AU - Hoff,Mariana Leivas Müller, AU - Klein,Roberta Daniele, AU - Cardozo,Janaina Goulart, AU - Giacomin,Marina Mussoi, AU - Pinho,Grasiela Lopes Leães, AU - Bianchini,Adalto, Y1 - 2013/04/29/ PY - 2012/12/29/received PY - 2013/04/12/revised PY - 2013/04/19/accepted PY - 2013/6/1/entrez PY - 2013/6/1/pubmed PY - 2013/10/18/medline SP - 60 EP - 9 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat. Toxicol. VL - 138-139 N2 - The responses of a large suite of biochemical and genetic parameters were evaluated in tissues (liver, gills, muscle and erythrocytes) of the estuarine guppy Poecilia vivipara exposed to waterborne copper in salt water (salinity 24 ppt). Activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase, and glutathione S-transferase), metallothionein-like protein concentration, reactive oxygen species (ROS) content, antioxidant capacity against peroxyl radicals (ACAP), and lipid peroxidation (LPO) were evaluated in liver, gills, and muscle. Comet assay score and nuclear abnormalities and micronucleated cell frequency were analyzed in peripheral erythrocytes. The responses of these parameters were evaluated in fish exposed (96 h) to environmentally relevant copper concentrations (5, 9 and 20 μg L⁻¹). In control and copper-exposed fish, no mortality was observed over the experimental period. Almost all biochemical and genetic parameters proved to be affected by waterborne copper exposure. However, the response of catalase activity in liver, ROS, ACAP and LPO in muscle, gills and liver, and DNA damages in erythrocytes clearly showed to be dependent on copper concentration in salt water. Therefore, the use of these parameters could be of relevance in the scope of biomonitoring programs in salt water environments contaminated with copper. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/23721848/Biomarkers_of_waterborne_copper_exposure_in_the_guppy_Poecilia_vivipara_acclimated_to_salt_water_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(13)00098-2 DB - PRIME DP - Unbound Medicine ER -