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Environmental concentrations of irgarol, diuron and S-metolachlor induce deleterious effects on gametes and embryos of the Pacific oyster, Crassostrea gigas.
Mar Environ Res. 2013 Aug; 89:1-8.ME

Abstract

Irgarol and diuron are the most representative "organic booster biocides" that replace organotin compounds in antifouling paints, and metolachlor is one of the most extensively used chloroacetamide herbicides in agriculture. The toxicity of S-metolachlor, irgarol and diuron was evaluated in Pacific oyster (Crassostrea gigas) gametes or embryos exposed to concentrations of pesticides ranging from 0.1× to 1000×, with 1× corresponding to environmental concentrations of the three studied pesticides in Arcachon Bay (France). Exposures were performed on (1) spermatozoa alone (2) oocytes alone and (3) both spermatozoa and oocytes, and adverse effects on fertilization success and offspring development were recorded. The results showed that the fertilizing capacity of spermatozoa was significantly affected after gamete exposure to pesticide concentrations as low as 1× of irgarol and diuron and 10× of metolachlor. The offspring obtained from pesticide-exposed spermatozoa displayed a dose-dependent increase in developmental abnormalities. In contrast, treating oocytes with pesticide concentrations up to 10× did not alter fertilization rate and offspring quality. However, a significant decline in fertilization success and increase in abnormal D-larvae prevalence were observed at higher concentrations 10× (0.1 μg L(-1)) for S-metolachlor and 100× for irgarol (1.0 μg L(-1)) and diuron (4.0 μg L(-1)). Irgarol, diuron and S-metolachlor also induced a dose-dependent increase in abnormal D-larvae prevalence when freshly fertilized embryos were treated with pesticide concentrations as low as concentration of 1× (0.01 μg L(-1) for irgarol or S-metolachlor, and 0.04 μg L(-1) for diuron). The two bioassays on C. gigas spermatozoa and embryos displayed similar sensitivities to the studied pesticides while oocytes were less sensitive. Diuron, irgarol and S-metolachlor induced spermiotoxicity and embryotoxicity at environmentally relevant concentrations and therefore might be a threat to oyster recruitment in coastal areas facing chronic inputs of pesticides.

Authors+Show Affiliations

Univ. Bordeaux, EPOC UMR CNRS 5805, F-33400 Talence, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23727205

Citation

Mai, Huong, et al. "Environmental Concentrations of Irgarol, Diuron and S-metolachlor Induce Deleterious Effects On Gametes and Embryos of the Pacific Oyster, Crassostrea Gigas." Marine Environmental Research, vol. 89, 2013, pp. 1-8.
Mai H, Morin B, Pardon P, et al. Environmental concentrations of irgarol, diuron and S-metolachlor induce deleterious effects on gametes and embryos of the Pacific oyster, Crassostrea gigas. Mar Environ Res. 2013;89:1-8.
Mai, H., Morin, B., Pardon, P., Gonzalez, P., Budzinski, H., & Cachot, J. (2013). Environmental concentrations of irgarol, diuron and S-metolachlor induce deleterious effects on gametes and embryos of the Pacific oyster, Crassostrea gigas. Marine Environmental Research, 89, 1-8. https://doi.org/10.1016/j.marenvres.2013.04.003
Mai H, et al. Environmental Concentrations of Irgarol, Diuron and S-metolachlor Induce Deleterious Effects On Gametes and Embryos of the Pacific Oyster, Crassostrea Gigas. Mar Environ Res. 2013;89:1-8. PubMed PMID: 23727205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Environmental concentrations of irgarol, diuron and S-metolachlor induce deleterious effects on gametes and embryos of the Pacific oyster, Crassostrea gigas. AU - Mai,Huong, AU - Morin,Bénédicte, AU - Pardon,Patrick, AU - Gonzalez,Patrice, AU - Budzinski,Hélène, AU - Cachot,Jérôme, Y1 - 2013/05/09/ PY - 2013/01/09/received PY - 2013/04/05/revised PY - 2013/04/18/accepted PY - 2013/6/4/entrez PY - 2013/6/4/pubmed PY - 2014/2/25/medline KW - Developmental abnormalities KW - Embryos KW - Fertilization success KW - Gametes KW - Pacific oyster KW - Pesticides SP - 1 EP - 8 JF - Marine environmental research JO - Mar Environ Res VL - 89 N2 - Irgarol and diuron are the most representative "organic booster biocides" that replace organotin compounds in antifouling paints, and metolachlor is one of the most extensively used chloroacetamide herbicides in agriculture. The toxicity of S-metolachlor, irgarol and diuron was evaluated in Pacific oyster (Crassostrea gigas) gametes or embryos exposed to concentrations of pesticides ranging from 0.1× to 1000×, with 1× corresponding to environmental concentrations of the three studied pesticides in Arcachon Bay (France). Exposures were performed on (1) spermatozoa alone (2) oocytes alone and (3) both spermatozoa and oocytes, and adverse effects on fertilization success and offspring development were recorded. The results showed that the fertilizing capacity of spermatozoa was significantly affected after gamete exposure to pesticide concentrations as low as 1× of irgarol and diuron and 10× of metolachlor. The offspring obtained from pesticide-exposed spermatozoa displayed a dose-dependent increase in developmental abnormalities. In contrast, treating oocytes with pesticide concentrations up to 10× did not alter fertilization rate and offspring quality. However, a significant decline in fertilization success and increase in abnormal D-larvae prevalence were observed at higher concentrations 10× (0.1 μg L(-1)) for S-metolachlor and 100× for irgarol (1.0 μg L(-1)) and diuron (4.0 μg L(-1)). Irgarol, diuron and S-metolachlor also induced a dose-dependent increase in abnormal D-larvae prevalence when freshly fertilized embryos were treated with pesticide concentrations as low as concentration of 1× (0.01 μg L(-1) for irgarol or S-metolachlor, and 0.04 μg L(-1) for diuron). The two bioassays on C. gigas spermatozoa and embryos displayed similar sensitivities to the studied pesticides while oocytes were less sensitive. Diuron, irgarol and S-metolachlor induced spermiotoxicity and embryotoxicity at environmentally relevant concentrations and therefore might be a threat to oyster recruitment in coastal areas facing chronic inputs of pesticides. SN - 1879-0291 UR - https://www.unboundmedicine.com/medline/citation/23727205/Environmental_concentrations_of_irgarol_diuron_and_S_metolachlor_induce_deleterious_effects_on_gametes_and_embryos_of_the_Pacific_oyster_Crassostrea_gigas_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-1136(13)00067-6 DB - PRIME DP - Unbound Medicine ER -