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Expression of rd29A::AtDREB1A/CBF3 in tomato alleviates drought-induced oxidative stress by regulating key enzymatic and non-enzymatic antioxidants.
Plant Physiol Biochem. 2013 Aug; 69:90-100.PP

Abstract

Transgenic tomato lines (cv. Kashi Vishesh) over-expressing AtDREB1A/CBF3 driven by stress-inducible rd29A promoter showed significantly higher activities of key antioxidant enzymes when exposed to water-deficit for 7, 14, and 21 days. Transgenic tomato plants exposed to water-deficit recorded lower levels of hydrogen peroxide and superoxide anion formation compared to the non-transgenic plants, suggesting alleviation of reactive oxygen species (ROS). A significant increase in activities of enzymes superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR), and monodehydroascorbate reductase (MDHAR) was observed in response to the different durations of water-deficit conditions. In contrast, enzyme guaiacol peroxidase (POD) activity was lower in the transgenic lines and showed a negative correlation with ROS, ascorbic acid (AsA), and glutathione levels. The concentrations of AsA, glutathione and their reduced forms were higher in the transgenic plants and increased with ROS levels. These results indicate that AtDREB1A transgenic tomato lines are better adapted to water-deficit as they showed lower drought-induced oxidative stress due to activation of the antioxidant response.

Authors+Show Affiliations

Indian Institute of Vegetable Research, P.O. Jakhini-Shahanshahpur, Varanasi 221 305, India. govindrai25@yahoo.inNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23728392

Citation

Rai, Govind Kumar, et al. "Expression of rd29A::AtDREB1A/CBF3 in Tomato Alleviates Drought-induced Oxidative Stress By Regulating Key Enzymatic and Non-enzymatic Antioxidants." Plant Physiology and Biochemistry : PPB, vol. 69, 2013, pp. 90-100.
Rai GK, Rai NP, Rathaur S, et al. Expression of rd29A::AtDREB1A/CBF3 in tomato alleviates drought-induced oxidative stress by regulating key enzymatic and non-enzymatic antioxidants. Plant Physiol Biochem. 2013;69:90-100.
Rai, G. K., Rai, N. P., Rathaur, S., Kumar, S., & Singh, M. (2013). Expression of rd29A::AtDREB1A/CBF3 in tomato alleviates drought-induced oxidative stress by regulating key enzymatic and non-enzymatic antioxidants. Plant Physiology and Biochemistry : PPB, 69, 90-100. https://doi.org/10.1016/j.plaphy.2013.05.002
Rai GK, et al. Expression of rd29A::AtDREB1A/CBF3 in Tomato Alleviates Drought-induced Oxidative Stress By Regulating Key Enzymatic and Non-enzymatic Antioxidants. Plant Physiol Biochem. 2013;69:90-100. PubMed PMID: 23728392.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of rd29A::AtDREB1A/CBF3 in tomato alleviates drought-induced oxidative stress by regulating key enzymatic and non-enzymatic antioxidants. AU - Rai,Govind Kumar, AU - Rai,Neha Prakash, AU - Rathaur,Sushma, AU - Kumar,Sanjeev, AU - Singh,Major, Y1 - 2013/05/15/ PY - 2012/12/28/received PY - 2013/05/03/accepted PY - 2013/6/4/entrez PY - 2013/6/4/pubmed PY - 2014/1/11/medline SP - 90 EP - 100 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 69 N2 - Transgenic tomato lines (cv. Kashi Vishesh) over-expressing AtDREB1A/CBF3 driven by stress-inducible rd29A promoter showed significantly higher activities of key antioxidant enzymes when exposed to water-deficit for 7, 14, and 21 days. Transgenic tomato plants exposed to water-deficit recorded lower levels of hydrogen peroxide and superoxide anion formation compared to the non-transgenic plants, suggesting alleviation of reactive oxygen species (ROS). A significant increase in activities of enzymes superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR), and monodehydroascorbate reductase (MDHAR) was observed in response to the different durations of water-deficit conditions. In contrast, enzyme guaiacol peroxidase (POD) activity was lower in the transgenic lines and showed a negative correlation with ROS, ascorbic acid (AsA), and glutathione levels. The concentrations of AsA, glutathione and their reduced forms were higher in the transgenic plants and increased with ROS levels. These results indicate that AtDREB1A transgenic tomato lines are better adapted to water-deficit as they showed lower drought-induced oxidative stress due to activation of the antioxidant response. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/23728392/Expression_of_rd29A::AtDREB1A/CBF3_in_tomato_alleviates_drought_induced_oxidative_stress_by_regulating_key_enzymatic_and_non_enzymatic_antioxidants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(13)00161-7 DB - PRIME DP - Unbound Medicine ER -