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Myricetin-mediated lifespan extension in Caenorhabditis elegans is modulated by DAF-16.
Int J Mol Sci 2013; 14(6):11895-914IJ

Abstract

Myricetin is a naturally occurring flavonol found in many plant based food sources. It increases the lifespan of Caenorhabditis elegans, but the molecular mechanisms are not yet fully understood. We have investigated the impact of this flavonoid on the transcription factors DAF-16 (C. elegans FoxO homologue) and SKN-1 (Nrf2 homologue), which have crucial functions in the regulation of ageing. Myricetin is rapidly assimilated by the nematode, causes a nuclear translocation of DAF-16 but not of SKN-1, and finally prolongs the mean adult lifespan of C. elegans by 32.9%. The lifespan prolongation was associated with a decrease in the accumulation of reactive oxygen species (ROS) detected by DCF. Myricetin also decreases the formation of lipofuscin, a pigment consisting of highly oxidized and cross-linked proteins that is considered as a biomarker of ageing in diverse species. The lifespan extension was completely abolished in a daf-16 loss-of-function mutant strain (CF1038). Consistently with this result, myricetin was also not able to diminish stress-induced ROS accumulation in the mutant. These results strongly indicate that the pro-longevity effect of myricetin is dependent on DAF-16 and not on direct anti-oxidative effects of the flavonoid.

Authors+Show Affiliations

Institute of Agricultural and Nutritional Sciences, Faculty III, Martin-Luther-Universität Halle-Wittenberg, Weinbergweg 22 (Biozentrum), 06120 Halle/Saale, Germany. wim.waetjen@landw.uni-halle.de.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23736695

Citation

Büchter, Christian, et al. "Myricetin-mediated Lifespan Extension in Caenorhabditis Elegans Is Modulated By DAF-16." International Journal of Molecular Sciences, vol. 14, no. 6, 2013, pp. 11895-914.
Büchter C, Ackermann D, Havermann S, et al. Myricetin-mediated lifespan extension in Caenorhabditis elegans is modulated by DAF-16. Int J Mol Sci. 2013;14(6):11895-914.
Büchter, C., Ackermann, D., Havermann, S., Honnen, S., Chovolou, Y., Fritz, G., ... Wätjen, W. (2013). Myricetin-mediated lifespan extension in Caenorhabditis elegans is modulated by DAF-16. International Journal of Molecular Sciences, 14(6), pp. 11895-914. doi:10.3390/ijms140611895.
Büchter C, et al. Myricetin-mediated Lifespan Extension in Caenorhabditis Elegans Is Modulated By DAF-16. Int J Mol Sci. 2013 Jun 4;14(6):11895-914. PubMed PMID: 23736695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myricetin-mediated lifespan extension in Caenorhabditis elegans is modulated by DAF-16. AU - Büchter,Christian, AU - Ackermann,Daniela, AU - Havermann,Susannah, AU - Honnen,Sebastian, AU - Chovolou,Yvonni, AU - Fritz,Gerhard, AU - Kampkötter,Andreas, AU - Wätjen,Wim, Y1 - 2013/06/04/ PY - 2013/04/26/received PY - 2013/05/16/revised PY - 2013/05/17/accepted PY - 2013/6/6/entrez PY - 2013/6/6/pubmed PY - 2013/6/6/medline SP - 11895 EP - 914 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 14 IS - 6 N2 - Myricetin is a naturally occurring flavonol found in many plant based food sources. It increases the lifespan of Caenorhabditis elegans, but the molecular mechanisms are not yet fully understood. We have investigated the impact of this flavonoid on the transcription factors DAF-16 (C. elegans FoxO homologue) and SKN-1 (Nrf2 homologue), which have crucial functions in the regulation of ageing. Myricetin is rapidly assimilated by the nematode, causes a nuclear translocation of DAF-16 but not of SKN-1, and finally prolongs the mean adult lifespan of C. elegans by 32.9%. The lifespan prolongation was associated with a decrease in the accumulation of reactive oxygen species (ROS) detected by DCF. Myricetin also decreases the formation of lipofuscin, a pigment consisting of highly oxidized and cross-linked proteins that is considered as a biomarker of ageing in diverse species. The lifespan extension was completely abolished in a daf-16 loss-of-function mutant strain (CF1038). Consistently with this result, myricetin was also not able to diminish stress-induced ROS accumulation in the mutant. These results strongly indicate that the pro-longevity effect of myricetin is dependent on DAF-16 and not on direct anti-oxidative effects of the flavonoid. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/23736695/Myricetin_mediated_lifespan_extension_in_Caenorhabditis_elegans_is_modulated_by_DAF_16_ L2 - http://www.mdpi.com/resolver?pii=ijms140611895 DB - PRIME DP - Unbound Medicine ER -