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Profibrotic interleukin-33 is correlated with uterine leiomyoma tumour burden.
Hum Reprod 2013; 28(8):2126-33HR

Abstract

STUDY QUESTION

Are interleukin-33 (IL-33) serum levels higher in women with uterine leiomyoma compared with controls without leiomyoma?

SUMMARY ANSWER

Serum IL-33 is elevated in women with uterine leiomyoma and correlated with features of uterine leiomyoma tumour burden, namely fibroid number, size and weight.

WHAT IS KNOWN ALREADY

Uterine leiomyomas are the most common benign tumours in premenopausal women associated with major tissue fibrosis. IL-33 is a cytokine involved in fibrotic disorders. The potential role of IL-33 in leiomyoma has not been reported before.

STUDY DESIGN, SIZE, DURATION

This is a prospective laboratory study conducted in a tertiary-care university hospital between January 2005 and December 2010. We investigated non-pregnant, 42-year-old patients (n = 151) during surgery for a benign gynaecological condition.

PARTICIPANTS/MATERIALS, SETTING, METHODS

After complete surgical exploration of the abdominopelvic cavity, 59 women with histologically proved uterine leiomyoma and 92 leiomyoma-free control women were enrolled. Women with endometriosis or past history of ovarian malignancy and borderline tumours were not included. The control group included women with benign ovarian cysts, paratubal cysts or tubal defects without any evidence of uterine leiomyoma. For each patient, a structured questionnaire was completed during a face-to-face interview conducted by the surgeon during the month preceding surgery. Serum samples were obtained in the month preceding the surgical procedures according to the menstrual phase or hormonal therapy. IL-33 was measured in sera by enzyme-linked immunosorbent assay, and correlation of IL-33 concentration with the extent and severity of the disease was investigated.

MAIN RESULTS AND THE ROLE OF CHANCE

IL-33 was detected in 32 (54.2%) women with leiomyoma and 18 (19.6%) controls (P < 0.001). Serum IL-33 was higher in women with leiomyoma (median, 140.1 pg/ml; range, 7.5-2247.7) than in controls (median, 27.8 pg/ml; range, 7.5-71.6; P = 0.002). We found positive correlations between serum IL-33 concentration and leiomyoma features, such as fibroid weight (r = 0.630; P = 0.001) and size (r = 0.511; P = 0.018) and the number of fibroids (r = 0.503; P = 0.003).

LIMITATIONS, REASONS FOR CAUTION

There was a possible selection bias due to inclusion of only surgical patients. Therefore our control group consisted of women who underwent surgery for benign gynaecological conditions. This may lead to biases stemming from the fact that certain of these conditions, such as tubal infertility or ovarian cysts, might be associated with altered serum IL-33 levels.

WIDER IMPLICATIONS OF THE FINDINGS

We demonstrate for the first time that elevated serum IL-33 levels are associated with the existence of uterine leiomyoma. However, even if an association does not constitute proof of cause and effect, investigating the mechanisms that underlie fibrogenesis associated with leiomyomas is a step towards understanding this enigmatic disease. This study opens the doors to future, more mechanistics studies to establish the exact role of IL-33 in uterine leiomyomas pathogenesis.

STUDY FUNDING/COMPETING INTEREST(S)

No funding, no conflict of interest.

Authors+Show Affiliations

Department of Gynecology Obstetrics II and Reproductive Medicine, Sorbonne Paris Cité, Faculté de Médecine, Université Paris Descartes, AP-HP, Hôpital Cochin, 75679 Paris, France. pietro.santulli@cch.aphp.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23739220

Citation

Santulli, Pietro, et al. "Profibrotic Interleukin-33 Is Correlated With Uterine Leiomyoma Tumour Burden." Human Reproduction (Oxford, England), vol. 28, no. 8, 2013, pp. 2126-33.
Santulli P, Even M, Chouzenoux S, et al. Profibrotic interleukin-33 is correlated with uterine leiomyoma tumour burden. Hum Reprod. 2013;28(8):2126-33.
Santulli, P., Even, M., Chouzenoux, S., Millischer, A. E., Borghese, B., de Ziegler, D., ... Chapron, C. (2013). Profibrotic interleukin-33 is correlated with uterine leiomyoma tumour burden. Human Reproduction (Oxford, England), 28(8), pp. 2126-33. doi:10.1093/humrep/det238.
Santulli P, et al. Profibrotic Interleukin-33 Is Correlated With Uterine Leiomyoma Tumour Burden. Hum Reprod. 2013;28(8):2126-33. PubMed PMID: 23739220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Profibrotic interleukin-33 is correlated with uterine leiomyoma tumour burden. AU - Santulli,Pietro, AU - Even,Marc, AU - Chouzenoux,Sandrine, AU - Millischer,Anne-Elodie, AU - Borghese,Bruno, AU - de Ziegler,Dominique, AU - Batteux,Frédéric, AU - Chapron,Charles, Y1 - 2013/06/05/ PY - 2013/6/7/entrez PY - 2013/6/7/pubmed PY - 2014/3/4/medline KW - fibroids KW - fibrosis KW - interleukin KW - interleukin-33 KW - pathogenesis SP - 2126 EP - 33 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 28 IS - 8 N2 - STUDY QUESTION: Are interleukin-33 (IL-33) serum levels higher in women with uterine leiomyoma compared with controls without leiomyoma? SUMMARY ANSWER: Serum IL-33 is elevated in women with uterine leiomyoma and correlated with features of uterine leiomyoma tumour burden, namely fibroid number, size and weight. WHAT IS KNOWN ALREADY: Uterine leiomyomas are the most common benign tumours in premenopausal women associated with major tissue fibrosis. IL-33 is a cytokine involved in fibrotic disorders. The potential role of IL-33 in leiomyoma has not been reported before. STUDY DESIGN, SIZE, DURATION: This is a prospective laboratory study conducted in a tertiary-care university hospital between January 2005 and December 2010. We investigated non-pregnant, 42-year-old patients (n = 151) during surgery for a benign gynaecological condition. PARTICIPANTS/MATERIALS, SETTING, METHODS: After complete surgical exploration of the abdominopelvic cavity, 59 women with histologically proved uterine leiomyoma and 92 leiomyoma-free control women were enrolled. Women with endometriosis or past history of ovarian malignancy and borderline tumours were not included. The control group included women with benign ovarian cysts, paratubal cysts or tubal defects without any evidence of uterine leiomyoma. For each patient, a structured questionnaire was completed during a face-to-face interview conducted by the surgeon during the month preceding surgery. Serum samples were obtained in the month preceding the surgical procedures according to the menstrual phase or hormonal therapy. IL-33 was measured in sera by enzyme-linked immunosorbent assay, and correlation of IL-33 concentration with the extent and severity of the disease was investigated. MAIN RESULTS AND THE ROLE OF CHANCE: IL-33 was detected in 32 (54.2%) women with leiomyoma and 18 (19.6%) controls (P < 0.001). Serum IL-33 was higher in women with leiomyoma (median, 140.1 pg/ml; range, 7.5-2247.7) than in controls (median, 27.8 pg/ml; range, 7.5-71.6; P = 0.002). We found positive correlations between serum IL-33 concentration and leiomyoma features, such as fibroid weight (r = 0.630; P = 0.001) and size (r = 0.511; P = 0.018) and the number of fibroids (r = 0.503; P = 0.003). LIMITATIONS, REASONS FOR CAUTION: There was a possible selection bias due to inclusion of only surgical patients. Therefore our control group consisted of women who underwent surgery for benign gynaecological conditions. This may lead to biases stemming from the fact that certain of these conditions, such as tubal infertility or ovarian cysts, might be associated with altered serum IL-33 levels. WIDER IMPLICATIONS OF THE FINDINGS: We demonstrate for the first time that elevated serum IL-33 levels are associated with the existence of uterine leiomyoma. However, even if an association does not constitute proof of cause and effect, investigating the mechanisms that underlie fibrogenesis associated with leiomyomas is a step towards understanding this enigmatic disease. This study opens the doors to future, more mechanistics studies to establish the exact role of IL-33 in uterine leiomyomas pathogenesis. STUDY FUNDING/COMPETING INTEREST(S): No funding, no conflict of interest. SN - 1460-2350 UR - https://www.unboundmedicine.com/medline/citation/23739220/Profibrotic_interleukin_33_is_correlated_with_uterine_leiomyoma_tumour_burden_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/det238 DB - PRIME DP - Unbound Medicine ER -