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Antibodies against thrombin in dengue patients contain both anti-thrombotic and pro-fibrinolytic activities.
Thromb Haemost. 2013 Aug; 110(2):358-65.TH

Abstract

Dengue virus (DENV) infection may result in severe life-threatening Dengue haemorrhagic fever (DHF). The mechanisms causing haemorrhage in those with DHF are unclear. In this study, we demonstrated that antibodies against human thrombin were increased in the sera of Dengue patients but not in that of patients infected with other viruses. To further characterise the properties of these antibodies, affinity-purified anti-thrombin antibodies (ATAs) were collected from Dengue patient sera by thrombin and protein A/L affinity columns. Most of the ATAs belonged to the IgG class and recognized DENV nonstructural protein 1 (NS1). In addition, we found that dengue patient ATAs also cross-reacted with human plasminogen (Plg). Functional studies in vitro indicated that Dengue patient ATAs could inhibit thrombin activity and enhance Plg activation. Taken together, these results suggest that DENV NS1-induced thrombin and Plg cross-reactive antibodies may contribute to the development of haemorrhage in patients with DHF by interfering with coagulation and fibrinolysis.

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Authors+Show Affiliations

Chuang YC
Department of Medical Laboratory Science and Biotechnology, Medical College, National Cheng Kung University, Tainan, Taiwan. today@mail.ncku.edu.tw
Lin YS
No affiliation info available
Liu HS
No affiliation info available
Wang JR
No affiliation info available
Yeh TM
No affiliation info available

MeSH

Antibodies, ViralAntigens, ViralAutoantibodiesBlood CoagulationCross ReactionsDengue VirusFibrinolysisHumansImmunoglobulin GPlasminogenProthrombinSevere DengueThrombinViral Nonstructural Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23740201

Citation

Chuang, Yung-Chun, et al. "Antibodies Against Thrombin in Dengue Patients Contain Both Anti-thrombotic and Pro-fibrinolytic Activities." Thrombosis and Haemostasis, vol. 110, no. 2, 2013, pp. 358-65.
Chuang YC, Lin YS, Liu HS, et al. Antibodies against thrombin in dengue patients contain both anti-thrombotic and pro-fibrinolytic activities. Thromb Haemost. 2013;110(2):358-65.
Chuang, Y. C., Lin, Y. S., Liu, H. S., Wang, J. R., & Yeh, T. M. (2013). Antibodies against thrombin in dengue patients contain both anti-thrombotic and pro-fibrinolytic activities. Thrombosis and Haemostasis, 110(2), 358-65. https://doi.org/10.1160/TH13-02-0149
Chuang YC, et al. Antibodies Against Thrombin in Dengue Patients Contain Both Anti-thrombotic and Pro-fibrinolytic Activities. Thromb Haemost. 2013;110(2):358-65. PubMed PMID: 23740201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibodies against thrombin in dengue patients contain both anti-thrombotic and pro-fibrinolytic activities. AU - Chuang,Yung-Chun, AU - Lin,Yee-Shin, AU - Liu,Hsiao-Sheng, AU - Wang,Jen-Reng, AU - Yeh,Trai-Ming, Y1 - 2013/06/06/ PY - 2013/02/22/received PY - 2013/05/02/accepted PY - 2013/6/7/entrez PY - 2013/6/7/pubmed PY - 2014/4/4/medline SP - 358 EP - 65 JF - Thrombosis and haemostasis JO - Thromb Haemost VL - 110 IS - 2 N2 - Dengue virus (DENV) infection may result in severe life-threatening Dengue haemorrhagic fever (DHF). The mechanisms causing haemorrhage in those with DHF are unclear. In this study, we demonstrated that antibodies against human thrombin were increased in the sera of Dengue patients but not in that of patients infected with other viruses. To further characterise the properties of these antibodies, affinity-purified anti-thrombin antibodies (ATAs) were collected from Dengue patient sera by thrombin and protein A/L affinity columns. Most of the ATAs belonged to the IgG class and recognized DENV nonstructural protein 1 (NS1). In addition, we found that dengue patient ATAs also cross-reacted with human plasminogen (Plg). Functional studies in vitro indicated that Dengue patient ATAs could inhibit thrombin activity and enhance Plg activation. Taken together, these results suggest that DENV NS1-induced thrombin and Plg cross-reactive antibodies may contribute to the development of haemorrhage in patients with DHF by interfering with coagulation and fibrinolysis. SN - 2567-689X UR - https://www.unboundmedicine.com/medline/citation/23740201/Antibodies_against_thrombin_in_dengue_patients_contain_both_anti_thrombotic_and_pro_fibrinolytic_activities_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1160/TH13-02-0149 DB - PRIME DP - Unbound Medicine ER -