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Correlation between human clusterin in seminal plasma with sperm protamine deficiency and DNA fragmentation.
Mol Reprod Dev. 2013 Sep; 80(9):718-24.MR

Abstract

Seminal proteins can be considered as factors that control fertilization. Clusterin is one such protein that has been implicated in many activities, including apoptosis inhibition, cell cycle control, DNA repair, and sperm maturation. In this study, the relationship between human secretory clusterin (sCLU) in seminal plasma with sperm parameters, protamine deficiency, and DNA fragmentation was investigated. Semen samples were collected from 63 Iranian men, and semen analysis was performed according to World Health Organization criteria and computer aided semen analysis (CASA). The concentration of sCLU in seminal plasma was measured by enzyme-linked immunosorbant assay (ELISA), protamine deficiency was determined by chromomycin A3 staining (CMA3), and sperm DNA fragmentation was checked by sperm chromatin dispersion (SCD) assay. The level of sCLU in seminal fluid of fertile patients was 48.3 ± 38.6 ng/ml and in infertile patients was 15.5 ± 9.7 ng/ml; this difference was significant (P < 0.001). sCLU correlated negatively with protamine deficiency, sperm DNA fragmentation, and abnormal morphology. In conclusion, seminal clusterin can be considered as a marker for the quick assessment of semen quality in male infertility studies.

Authors+Show Affiliations

Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23740886

Citation

Salehi, Mohammad, et al. "Correlation Between Human Clusterin in Seminal Plasma With Sperm Protamine Deficiency and DNA Fragmentation." Molecular Reproduction and Development, vol. 80, no. 9, 2013, pp. 718-24.
Salehi M, Akbari H, Heidari MH, et al. Correlation between human clusterin in seminal plasma with sperm protamine deficiency and DNA fragmentation. Mol Reprod Dev. 2013;80(9):718-24.
Salehi, M., Akbari, H., Heidari, M. H., Molouki, A., Murulitharan, K., Moeini, H., Novin, M. G., Aabed, F., Taheri, H., Fadaei, F., Mohsenzadeh, M., Jafari, M., Pirouzi, A., & Heidari, R. (2013). Correlation between human clusterin in seminal plasma with sperm protamine deficiency and DNA fragmentation. Molecular Reproduction and Development, 80(9), 718-24. https://doi.org/10.1002/mrd.22202
Salehi M, et al. Correlation Between Human Clusterin in Seminal Plasma With Sperm Protamine Deficiency and DNA Fragmentation. Mol Reprod Dev. 2013;80(9):718-24. PubMed PMID: 23740886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation between human clusterin in seminal plasma with sperm protamine deficiency and DNA fragmentation. AU - Salehi,Mohammad, AU - Akbari,Hakimeh, AU - Heidari,Mohammad Hassan, AU - Molouki,Aidin, AU - Murulitharan,Kavitha, AU - Moeini,Hassan, AU - Novin,Marefat Ghaffari, AU - Aabed,Farhang, AU - Taheri,Hossein, AU - Fadaei,Fateme, AU - Mohsenzadeh,Mehdi, AU - Jafari,Mohammad, AU - Pirouzi,Aliyar, AU - Heidari,Reihane, Y1 - 2013/07/01/ PY - 2012/12/19/received PY - 2013/05/30/accepted PY - 2013/6/7/entrez PY - 2013/6/7/pubmed PY - 2014/5/3/medline SP - 718 EP - 24 JF - Molecular reproduction and development JO - Mol. Reprod. Dev. VL - 80 IS - 9 N2 - Seminal proteins can be considered as factors that control fertilization. Clusterin is one such protein that has been implicated in many activities, including apoptosis inhibition, cell cycle control, DNA repair, and sperm maturation. In this study, the relationship between human secretory clusterin (sCLU) in seminal plasma with sperm parameters, protamine deficiency, and DNA fragmentation was investigated. Semen samples were collected from 63 Iranian men, and semen analysis was performed according to World Health Organization criteria and computer aided semen analysis (CASA). The concentration of sCLU in seminal plasma was measured by enzyme-linked immunosorbant assay (ELISA), protamine deficiency was determined by chromomycin A3 staining (CMA3), and sperm DNA fragmentation was checked by sperm chromatin dispersion (SCD) assay. The level of sCLU in seminal fluid of fertile patients was 48.3 ± 38.6 ng/ml and in infertile patients was 15.5 ± 9.7 ng/ml; this difference was significant (P < 0.001). sCLU correlated negatively with protamine deficiency, sperm DNA fragmentation, and abnormal morphology. In conclusion, seminal clusterin can be considered as a marker for the quick assessment of semen quality in male infertility studies. SN - 1098-2795 UR - https://www.unboundmedicine.com/medline/citation/23740886/Correlation_between_human_clusterin_in_seminal_plasma_with_sperm_protamine_deficiency_and_DNA_fragmentation_ L2 - https://doi.org/10.1002/mrd.22202 DB - PRIME DP - Unbound Medicine ER -