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Rice OsGL1-6 is involved in leaf cuticular wax accumulation and drought resistance.
PLoS One. 2013; 8(5):e65139.Plos

Abstract

Cuticular wax is a class of organic compounds that comprises the outermost layer of plant surfaces. Plant cuticular wax, the last barrier of self-defense, plays an important role in plant growth and development. The OsGL1-6 gene, a member of the fatty aldehyde decarbonylase gene family, is highly homologous to Arabidopsis CER1, which is involved in cuticular wax biosynthesis. However, whether OsGL1-6 participates in cuticular wax biosynthesis remains unknown. In this study, an OsGL1-6 antisense-RNA vector driven by its own promoter was constructed and introduced into the rice variety Zhonghua11 by Agrobacterium-mediated transformation to obtain several independent transgenic plants with decreased OsGL1-6 expression. These OsGL1-6 antisense-RNA transgenic plants showed droopy leaves at the booting stage, significantly decreased leaf cuticular wax deposition, thinner cuticle membrane, increased chlorophyll leaching and water loss rates, and enhanced drought sensitivity. The OsGL1-6 gene was constitutively expressed in all examined organs and was very highly expressed in leaf epidermal cells and vascular bundles. The transient expression of OsGL1-6-GFP fusion indicated that OsGL1-6 is localized in the endoplasmic reticulum. Qualitative and quantitative analysis of the wax composition using gas chromatography-mass spectrometry revealed a significantly reduced total cuticular wax load on the leaf blades of the OsGL1-6 antisense-RNA transgenic plants as well as markedly decreased alkane and aldehyde contents. Their primary alcohol contents increased significantly compared with those in the wild type plants, suggesting that OsGL1-6 is associated with the decarbonylation pathways in wax biosynthesis. We propose that OsGL1-6 is involved in the accumulation of leaf cuticular wax and directly impacts drought resistance in rice.

Authors+Show Affiliations

Laboratory Center of Basic Biology and Biotechnology, Education Department of Guangdong Province, College of Life Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23741473

Citation

Zhou, Lingyan, et al. "Rice OsGL1-6 Is Involved in Leaf Cuticular Wax Accumulation and Drought Resistance." PloS One, vol. 8, no. 5, 2013, pp. e65139.
Zhou L, Ni E, Yang J, et al. Rice OsGL1-6 is involved in leaf cuticular wax accumulation and drought resistance. PLoS One. 2013;8(5):e65139.
Zhou, L., Ni, E., Yang, J., Zhou, H., Liang, H., Li, J., Jiang, D., Wang, Z., Liu, Z., & Zhuang, C. (2013). Rice OsGL1-6 is involved in leaf cuticular wax accumulation and drought resistance. PloS One, 8(5), e65139. https://doi.org/10.1371/journal.pone.0065139
Zhou L, et al. Rice OsGL1-6 Is Involved in Leaf Cuticular Wax Accumulation and Drought Resistance. PLoS One. 2013;8(5):e65139. PubMed PMID: 23741473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rice OsGL1-6 is involved in leaf cuticular wax accumulation and drought resistance. AU - Zhou,Lingyan, AU - Ni,Erdong, AU - Yang,Jiawei, AU - Zhou,Hai, AU - Liang,Hong, AU - Li,Jing, AU - Jiang,Dagang, AU - Wang,Zhonghua, AU - Liu,Zhenlan, AU - Zhuang,Chuxiong, Y1 - 2013/05/31/ PY - 2012/12/14/received PY - 2013/04/23/accepted PY - 2013/6/7/entrez PY - 2013/6/7/pubmed PY - 2014/1/11/medline SP - e65139 EP - e65139 JF - PloS one JO - PLoS One VL - 8 IS - 5 N2 - Cuticular wax is a class of organic compounds that comprises the outermost layer of plant surfaces. Plant cuticular wax, the last barrier of self-defense, plays an important role in plant growth and development. The OsGL1-6 gene, a member of the fatty aldehyde decarbonylase gene family, is highly homologous to Arabidopsis CER1, which is involved in cuticular wax biosynthesis. However, whether OsGL1-6 participates in cuticular wax biosynthesis remains unknown. In this study, an OsGL1-6 antisense-RNA vector driven by its own promoter was constructed and introduced into the rice variety Zhonghua11 by Agrobacterium-mediated transformation to obtain several independent transgenic plants with decreased OsGL1-6 expression. These OsGL1-6 antisense-RNA transgenic plants showed droopy leaves at the booting stage, significantly decreased leaf cuticular wax deposition, thinner cuticle membrane, increased chlorophyll leaching and water loss rates, and enhanced drought sensitivity. The OsGL1-6 gene was constitutively expressed in all examined organs and was very highly expressed in leaf epidermal cells and vascular bundles. The transient expression of OsGL1-6-GFP fusion indicated that OsGL1-6 is localized in the endoplasmic reticulum. Qualitative and quantitative analysis of the wax composition using gas chromatography-mass spectrometry revealed a significantly reduced total cuticular wax load on the leaf blades of the OsGL1-6 antisense-RNA transgenic plants as well as markedly decreased alkane and aldehyde contents. Their primary alcohol contents increased significantly compared with those in the wild type plants, suggesting that OsGL1-6 is associated with the decarbonylation pathways in wax biosynthesis. We propose that OsGL1-6 is involved in the accumulation of leaf cuticular wax and directly impacts drought resistance in rice. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23741473/Rice_OsGL1_6_is_involved_in_leaf_cuticular_wax_accumulation_and_drought_resistance_ L2 - https://dx.plos.org/10.1371/journal.pone.0065139 DB - PRIME DP - Unbound Medicine ER -