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Rapid determination of hexapeptides by hydrophilic interaction LC-MS/MS for in vitro skin-penetration studies.
Bioanalysis. 2013 Jun; 5(11):1353-62.B

Abstract

BACKGROUND

A sensitive analytical method is needed for assessing penetration of topically applied peptides for in vitro skin-penetration studies.

RESULTS

A rapid hydrophilic interaction LC (HILIC)-MS/MS method for analyzing the polar peptides Ac-EEMQRR-amide and H2N-EEMQRR-amide in various skin layers and matrices has been developed and evaluated. The matrices included emulsion, receptor fluids, cotton-tipped applicators, stratum corneum tape strips, epidermis and dermis of the skin. Stable isotopically labeled analogues were used as internal standards to correct for recovery and matrix effects. A HILIC-SPE procedure was optimized to minimize significant ion suppression in the more complex matrices.

CONCLUSION

This HILIC-MS/MS method is applicable to the determination of Ac-EEMQRR-amide and H2N-EEMQRR-amide in complex skin samples and other matrices generated during in vitro skin-penetration studies.

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Authors+Show Affiliations

Zhou W
US FDA, Center for Food Safety & Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA.
Wang PG
No affiliation info available
Ogunsola OA
No affiliation info available
Kraeling ME
No affiliation info available

MeSH

Amino Acid SequenceAnimalsChromatography, LiquidGuinea PigsHumansHydrophobic and Hydrophilic InteractionsLimit of DetectionOligopeptidesSkinSkin AbsorptionTandem Mass SpectrometryTime Factors

Pub Type(s)

Evaluation Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

23742305

Citation

Zhou, Wanlong, et al. "Rapid Determination of Hexapeptides By Hydrophilic Interaction LC-MS/MS for in Vitro Skin-penetration Studies." Bioanalysis, vol. 5, no. 11, 2013, pp. 1353-62.
Zhou W, Wang PG, Ogunsola OA, et al. Rapid determination of hexapeptides by hydrophilic interaction LC-MS/MS for in vitro skin-penetration studies. Bioanalysis. 2013;5(11):1353-62.
Zhou, W., Wang, P. G., Ogunsola, O. A., & Kraeling, M. E. (2013). Rapid determination of hexapeptides by hydrophilic interaction LC-MS/MS for in vitro skin-penetration studies. Bioanalysis, 5(11), 1353-62. https://doi.org/10.4155/bio.13.60
Zhou W, et al. Rapid Determination of Hexapeptides By Hydrophilic Interaction LC-MS/MS for in Vitro Skin-penetration Studies. Bioanalysis. 2013;5(11):1353-62. PubMed PMID: 23742305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid determination of hexapeptides by hydrophilic interaction LC-MS/MS for in vitro skin-penetration studies. AU - Zhou,Wanlong, AU - Wang,Perry G, AU - Ogunsola,Oluwatosin A, AU - Kraeling,Margaret E K, PY - 2013/6/8/entrez PY - 2013/6/8/pubmed PY - 2013/12/24/medline SP - 1353 EP - 62 JF - Bioanalysis JO - Bioanalysis VL - 5 IS - 11 N2 - BACKGROUND: A sensitive analytical method is needed for assessing penetration of topically applied peptides for in vitro skin-penetration studies. RESULTS: A rapid hydrophilic interaction LC (HILIC)-MS/MS method for analyzing the polar peptides Ac-EEMQRR-amide and H2N-EEMQRR-amide in various skin layers and matrices has been developed and evaluated. The matrices included emulsion, receptor fluids, cotton-tipped applicators, stratum corneum tape strips, epidermis and dermis of the skin. Stable isotopically labeled analogues were used as internal standards to correct for recovery and matrix effects. A HILIC-SPE procedure was optimized to minimize significant ion suppression in the more complex matrices. CONCLUSION: This HILIC-MS/MS method is applicable to the determination of Ac-EEMQRR-amide and H2N-EEMQRR-amide in complex skin samples and other matrices generated during in vitro skin-penetration studies. SN - 1757-6199 UR - https://www.unboundmedicine.com/medline/citation/23742305/Rapid_determination_of_hexapeptides_by_hydrophilic_interaction_LC_MS/MS_for_in_vitro_skin_penetration_studies_ L2 - https://www.future-science.com/doi/10.4155/bio.13.60?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -
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