Tags

Type your tag names separated by a space and hit enter

Enhanced phytotransformation of Navy Blue RX dye by Petunia grandiflora Juss. with augmentation of rhizospheric Bacillus pumilus strain PgJ and subsequent toxicity analysis.
Bioresour Technol. 2013 Aug; 142:246-54.BT

Abstract

This study reveals the beneficial synergistic phytoremediation potential of Petunia grandiflora Juss. with its rhizospheric bacterial isolate Bacillus pumilus strain PgJ to decolorize reactive Navy Blue RX (NBRX) dye by their active enzymatic machinery. In vitro cultures of P. grandiflora and B. pumilus gave 80.01% and 76.80% while their consortium decolorized NBRX up to 96.86% within 36 h. Significant induction in the enzyme activities of lignin peroxidase (207%), tyrosinase (133%), laccase (161%), riboflavin reductase (78%) were seen in the roots of tissue cultured plants while enzymes tyrosinase (660%), laccase (689%), riboflavin reductase (528%) were induced significantly in the B. pumilus cells. Metabolites of treated NBRX were analyzed using UV-vis spectroscopy, gas chromatography and biotransformation was visualized using high performance thin layer chromatography profile. Metabolites of the dye exhibited reduced phytotoxicity Sorghum vulgare and Phaeseolus mungo and significant reduction in cytogenotoxicity on Allium cepa roots when compared to NBRX.

Links

Publisher Full Text

Authors+Show Affiliations

Watharkar AD
Department of Biotechnology, Shivaji University, Kolhapur, India.
Rane NR
No affiliation info available
Patil SM
No affiliation info available
Khandare RV
No affiliation info available
Jadhav JP
No affiliation info available

MeSH

BacillusBiotransformationChromatography, GasChromatography, Thin LayerColorColoring AgentsComet AssayEnzyme InductionLaccaseMonophenol MonooxygenasePeroxidasesPetuniaPhylogenySpectrophotometry, UltravioletToxicity Tests

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23743429

Citation

Watharkar, Anuprita D., et al. "Enhanced Phytotransformation of Navy Blue RX Dye By Petunia Grandiflora Juss. With Augmentation of Rhizospheric Bacillus Pumilus Strain PgJ and Subsequent Toxicity Analysis." Bioresource Technology, vol. 142, 2013, pp. 246-54.
Watharkar AD, Rane NR, Patil SM, et al. Enhanced phytotransformation of Navy Blue RX dye by Petunia grandiflora Juss. with augmentation of rhizospheric Bacillus pumilus strain PgJ and subsequent toxicity analysis. Bioresour Technol. 2013;142:246-54.
Watharkar, A. D., Rane, N. R., Patil, S. M., Khandare, R. V., & Jadhav, J. P. (2013). Enhanced phytotransformation of Navy Blue RX dye by Petunia grandiflora Juss. with augmentation of rhizospheric Bacillus pumilus strain PgJ and subsequent toxicity analysis. Bioresource Technology, 142, 246-54. https://doi.org/10.1016/j.biortech.2013.05.044
Watharkar AD, et al. Enhanced Phytotransformation of Navy Blue RX Dye By Petunia Grandiflora Juss. With Augmentation of Rhizospheric Bacillus Pumilus Strain PgJ and Subsequent Toxicity Analysis. Bioresour Technol. 2013;142:246-54. PubMed PMID: 23743429.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced phytotransformation of Navy Blue RX dye by Petunia grandiflora Juss. with augmentation of rhizospheric Bacillus pumilus strain PgJ and subsequent toxicity analysis. AU - Watharkar,Anuprita D, AU - Rane,Niraj R, AU - Patil,Swapnil M, AU - Khandare,Rahul V, AU - Jadhav,Jyoti P, Y1 - 2013/05/22/ PY - 2013/04/03/received PY - 2013/05/13/revised PY - 2013/05/15/accepted PY - 2013/6/8/entrez PY - 2013/6/8/pubmed PY - 2014/2/12/medline KW - Cytogenotoxicity KW - Decolorization KW - Navy Blue RX KW - Phytoremediation KW - Plant-bacterium consortium SP - 246 EP - 54 JF - Bioresource technology JO - Bioresour Technol VL - 142 N2 - This study reveals the beneficial synergistic phytoremediation potential of Petunia grandiflora Juss. with its rhizospheric bacterial isolate Bacillus pumilus strain PgJ to decolorize reactive Navy Blue RX (NBRX) dye by their active enzymatic machinery. In vitro cultures of P. grandiflora and B. pumilus gave 80.01% and 76.80% while their consortium decolorized NBRX up to 96.86% within 36 h. Significant induction in the enzyme activities of lignin peroxidase (207%), tyrosinase (133%), laccase (161%), riboflavin reductase (78%) were seen in the roots of tissue cultured plants while enzymes tyrosinase (660%), laccase (689%), riboflavin reductase (528%) were induced significantly in the B. pumilus cells. Metabolites of treated NBRX were analyzed using UV-vis spectroscopy, gas chromatography and biotransformation was visualized using high performance thin layer chromatography profile. Metabolites of the dye exhibited reduced phytotoxicity Sorghum vulgare and Phaeseolus mungo and significant reduction in cytogenotoxicity on Allium cepa roots when compared to NBRX. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/23743429/Enhanced_phytotransformation_of_Navy_Blue_RX_dye_by_Petunia_grandiflora_Juss__with_augmentation_of_rhizospheric_Bacillus_pumilus_strain_PgJ_and_subsequent_toxicity_analysis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(13)00804-3 DB - PRIME DP - Unbound Medicine ER -