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Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus.
J Nat Med. 2014 Jan; 68(1):225-30.JN

Abstract

A quantitative (1)H-NMR method (qHNMR) was used to measure the amygdalin content of Persicae semen, Armeniacae semen, and Mume fructus, in each of which amygdalin constitutes a major component. The purity of amygdalin was calculated from the ratio of the intensity of the amygdalin H-2 signal at δ 6.50 ppm in pyridine-d 5 to that of the hexamethyldisilane (HMD) signal at 0 ppm. The HMD concentration was corrected by the International System of Units (SI) traceability with certified reference material (CRM)-grade bisphenol A. qHNMR revealed the amygdalin contents to be 2.72 and 3.13% in 2 lots of Persicae semen, 3.62 and 5.19% in 2 lots of Armeniacae semen, and 0.23% in Mume fructus. Thus, we demonstrated the utility of this method for the quantitative analysis of crude drugs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Tanaka R
Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.
Nitta A
No affiliation info available
Nagatsu A
No affiliation info available

MeSH

AmygdalinCalibrationChromatography, High Pressure LiquidMagnetic Resonance SpectroscopyPhytotherapyPlant PreparationsPlants, MedicinalPrunusReference Standards

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23744252

Citation

Tanaka, Rie, et al. "Application of a Quantitative 1H-NMR Method for the Determination of Amygdalin in Persicae Semen, Armeniacae Semen, and Mume Fructus." Journal of Natural Medicines, vol. 68, no. 1, 2014, pp. 225-30.
Tanaka R, Nitta A, Nagatsu A. Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus. J Nat Med. 2014;68(1):225-30.
Tanaka, R., Nitta, A., & Nagatsu, A. (2014). Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus. Journal of Natural Medicines, 68(1), 225-30. https://doi.org/10.1007/s11418-013-0783-y
Tanaka R, Nitta A, Nagatsu A. Application of a Quantitative 1H-NMR Method for the Determination of Amygdalin in Persicae Semen, Armeniacae Semen, and Mume Fructus. J Nat Med. 2014;68(1):225-30. PubMed PMID: 23744252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus. AU - Tanaka,Rie, AU - Nitta,Akane, AU - Nagatsu,Akito, Y1 - 2013/06/07/ PY - 2013/04/15/received PY - 2013/05/06/accepted PY - 2013/6/8/entrez PY - 2013/6/8/pubmed PY - 2014/5/23/medline SP - 225 EP - 30 JF - Journal of natural medicines JO - J Nat Med VL - 68 IS - 1 N2 - A quantitative (1)H-NMR method (qHNMR) was used to measure the amygdalin content of Persicae semen, Armeniacae semen, and Mume fructus, in each of which amygdalin constitutes a major component. The purity of amygdalin was calculated from the ratio of the intensity of the amygdalin H-2 signal at δ 6.50 ppm in pyridine-d 5 to that of the hexamethyldisilane (HMD) signal at 0 ppm. The HMD concentration was corrected by the International System of Units (SI) traceability with certified reference material (CRM)-grade bisphenol A. qHNMR revealed the amygdalin contents to be 2.72 and 3.13% in 2 lots of Persicae semen, 3.62 and 5.19% in 2 lots of Armeniacae semen, and 0.23% in Mume fructus. Thus, we demonstrated the utility of this method for the quantitative analysis of crude drugs. SN - 1861-0293 UR - https://www.unboundmedicine.com/medline/citation/23744252/Application_of_a_quantitative_1H_NMR_method_for_the_determination_of_amygdalin_in_Persicae_semen_Armeniacae_semen_and_Mume_fructus_ L2 - https://dx.doi.org/10.1007/s11418-013-0783-y DB - PRIME DP - Unbound Medicine ER -