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Changes in pain behavior and glial activation in the spinal dorsal horn after pulsed radiofrequency current administration to the dorsal root ganglion in a rat model of lumbar disc herniation: laboratory investigation.
J Neurosurg Spine. 2013 Aug; 19(2):256-63.JN

Abstract

OBJECT

Herniated discs can induce sciatica by mechanical compression and/or chemical irritation caused by proinflammatory cytokines. Using immunohistochemistry methods in the dorsal horn of a rat model of lumbar disc herniation, the authors investigated the effects of pulsed radiofrequency (PRF) current administration to the dorsal root ganglion (DRG) on pain-related behavior and activation of microglia, astrocytes, and mitogen-activated protein kinase.

METHODS

A total of 33 Sprague-Dawley rats were randomly assigned to either a sham-operated group (n = 10) or a nucleus pulposus (NP)-exposed group (n = 23). Rats in the NP-exposed group were further subdivided into NP exposed with sham stimulation (NP+sham stimulation, n = 10), NP exposed with PRF (NP+PRF, n = 10), or euthanasia 10 days after NP exposure (n = 3). The DRGs in the NP+PRF rats were exposed to PRF waves (2 Hz) for 120 seconds at 45 V on postoperative Day 10. Rats were tested for mechanical allodynia 10 days after surgery and at 8 hours, 1 day, 3 days, 10 days, 20 days, and 40 days after PRF administration. Immunohistochemical staining of astrocytes (glial fibrillary acidic protein), microglia (OX-42), and phosphorylated extracellular signal-regulated kinases (pERKs) in the spinal dorsal horn was performed at 41 days after PRF administration.

RESULTS

Starting at 8 hours after PRF administration, mechanical withdrawal thresholds dramatically increased; this response persisted for 40 days (p < 0.05). After PRF administration, immunohistochemical expressions of OX-42 and pERK in the spinal dorsal horn were quantitatively reduced (p < 0.05).

CONCLUSIONS

Pulsed radiofrequency administration to the DRG reduced mechanical allodynia and downregulated microglia activity and pERK expression in the spinal dorsal horn of a rat model of lumbar disc herniation.

Authors+Show Affiliations

Department of Physical Medicine and Rehabilitation, College of Medicine, Yeungnam University, Daegu, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23746090

Citation

Cho, Hee Kyung, et al. "Changes in Pain Behavior and Glial Activation in the Spinal Dorsal Horn After Pulsed Radiofrequency Current Administration to the Dorsal Root Ganglion in a Rat Model of Lumbar Disc Herniation: Laboratory Investigation." Journal of Neurosurgery. Spine, vol. 19, no. 2, 2013, pp. 256-63.
Cho HK, Cho YW, Kim EH, et al. Changes in pain behavior and glial activation in the spinal dorsal horn after pulsed radiofrequency current administration to the dorsal root ganglion in a rat model of lumbar disc herniation: laboratory investigation. J Neurosurg Spine. 2013;19(2):256-63.
Cho, H. K., Cho, Y. W., Kim, E. H., Sluijter, M. E., Hwang, S. J., & Ahn, S. H. (2013). Changes in pain behavior and glial activation in the spinal dorsal horn after pulsed radiofrequency current administration to the dorsal root ganglion in a rat model of lumbar disc herniation: laboratory investigation. Journal of Neurosurgery. Spine, 19(2), 256-63. https://doi.org/10.3171/2013.5.SPINE12731
Cho HK, et al. Changes in Pain Behavior and Glial Activation in the Spinal Dorsal Horn After Pulsed Radiofrequency Current Administration to the Dorsal Root Ganglion in a Rat Model of Lumbar Disc Herniation: Laboratory Investigation. J Neurosurg Spine. 2013;19(2):256-63. PubMed PMID: 23746090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in pain behavior and glial activation in the spinal dorsal horn after pulsed radiofrequency current administration to the dorsal root ganglion in a rat model of lumbar disc herniation: laboratory investigation. AU - Cho,Hee Kyung, AU - Cho,Yun Woo, AU - Kim,Eun Hyuk, AU - Sluijter,Menno E, AU - Hwang,Se Jin, AU - Ahn,Sang Ho, Y1 - 2013/06/07/ PY - 2013/6/11/entrez PY - 2013/6/12/pubmed PY - 2013/10/18/medline SP - 256 EP - 63 JF - Journal of neurosurgery. Spine JO - J Neurosurg Spine VL - 19 IS - 2 N2 - OBJECT: Herniated discs can induce sciatica by mechanical compression and/or chemical irritation caused by proinflammatory cytokines. Using immunohistochemistry methods in the dorsal horn of a rat model of lumbar disc herniation, the authors investigated the effects of pulsed radiofrequency (PRF) current administration to the dorsal root ganglion (DRG) on pain-related behavior and activation of microglia, astrocytes, and mitogen-activated protein kinase. METHODS: A total of 33 Sprague-Dawley rats were randomly assigned to either a sham-operated group (n = 10) or a nucleus pulposus (NP)-exposed group (n = 23). Rats in the NP-exposed group were further subdivided into NP exposed with sham stimulation (NP+sham stimulation, n = 10), NP exposed with PRF (NP+PRF, n = 10), or euthanasia 10 days after NP exposure (n = 3). The DRGs in the NP+PRF rats were exposed to PRF waves (2 Hz) for 120 seconds at 45 V on postoperative Day 10. Rats were tested for mechanical allodynia 10 days after surgery and at 8 hours, 1 day, 3 days, 10 days, 20 days, and 40 days after PRF administration. Immunohistochemical staining of astrocytes (glial fibrillary acidic protein), microglia (OX-42), and phosphorylated extracellular signal-regulated kinases (pERKs) in the spinal dorsal horn was performed at 41 days after PRF administration. RESULTS: Starting at 8 hours after PRF administration, mechanical withdrawal thresholds dramatically increased; this response persisted for 40 days (p < 0.05). After PRF administration, immunohistochemical expressions of OX-42 and pERK in the spinal dorsal horn were quantitatively reduced (p < 0.05). CONCLUSIONS: Pulsed radiofrequency administration to the DRG reduced mechanical allodynia and downregulated microglia activity and pERK expression in the spinal dorsal horn of a rat model of lumbar disc herniation. SN - 1547-5646 UR - https://www.unboundmedicine.com/medline/citation/23746090/Changes_in_pain_behavior_and_glial_activation_in_the_spinal_dorsal_horn_after_pulsed_radiofrequency_current_administration_to_the_dorsal_root_ganglion_in_a_rat_model_of_lumbar_disc_herniation:_laboratory_investigation_ L2 - https://thejns.org/doi/10.3171/2013.5.SPINE12731 DB - PRIME DP - Unbound Medicine ER -