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Snake venomics of Lachesis muta rhombeata and genus-wide antivenomics assessment of the paraspecific immunoreactivity of two antivenoms evidence the high compositional and immunological conservation across Lachesis.
J Proteomics. 2013 Aug 26; 89:112-23.JP

Abstract

We report the proteomic analysis of the Atlantic bushmaster, Lachesis muta rhombeata, from Brazil. Along with previous characterization of the venom proteomes of L. stenophrys (Costa Rica), L. melanocephala (Costa Rica), L. acrochorda (Colombia), and L. muta muta (Bolivia), the present study provides the first overview of the composition and distribution of venom proteins across this wide-ranging genus, and highlights the remarkable similar compositional and pharmacological profiles across Lachesis venoms. The paraspecificity of two antivenoms, produced at Instituto Vital Brazil (Brazil) and Instituto Clodomiro Picado (Costa Rica) using different conspecific taxa in the immunization mixtures, was assessed using genus-wide comparative antivenomics. This study confirms that the proteomic similarity among Lachesis sp. venoms is mirrored in their high immunological conservation across the genus. The clinical and therapeutic consequences of genus-wide venomics and antivenomics investigations of Lachesis venoms are discussed.

BIOLOGICAL SIGNIFICANCE

The proteomics characterization of L. m. rhombeata venom completes the overview of Lachesis venom proteomes and confirms the remarkable toxin profile conservation across the five clades of this wide-ranging genus. Genus-wide antivenomics showed that two antivenoms, produced against L. stenophrys or L. m. rhombeata, exhibit paraspecificity towards all other congeneric venoms. Our venomics study shows that, despite the broad geographic distribution of the genus, monospecific antivenoms may achieve clinical coverage for any Lachesis sp. envenoming.

Authors+Show Affiliations

Laboratorio de Venómica y Proteinómica Estructural, Instituto de Biomedicina de Valencia, C.S.I.C., Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23747394

Citation

Pla, Davinia, et al. "Snake Venomics of Lachesis Muta Rhombeata and Genus-wide Antivenomics Assessment of the Paraspecific Immunoreactivity of Two Antivenoms Evidence the High Compositional and Immunological Conservation Across Lachesis." Journal of Proteomics, vol. 89, 2013, pp. 112-23.
Pla D, Sanz L, Molina-Sánchez P, et al. Snake venomics of Lachesis muta rhombeata and genus-wide antivenomics assessment of the paraspecific immunoreactivity of two antivenoms evidence the high compositional and immunological conservation across Lachesis. J Proteomics. 2013;89:112-23.
Pla, D., Sanz, L., Molina-Sánchez, P., Zorita, V., Madrigal, M., Flores-Díaz, M., Alape-Girón, A., Núñez, V., Andrés, V., Gutiérrez, J. M., & Calvete, J. J. (2013). Snake venomics of Lachesis muta rhombeata and genus-wide antivenomics assessment of the paraspecific immunoreactivity of two antivenoms evidence the high compositional and immunological conservation across Lachesis. Journal of Proteomics, 89, 112-23. https://doi.org/10.1016/j.jprot.2013.05.028
Pla D, et al. Snake Venomics of Lachesis Muta Rhombeata and Genus-wide Antivenomics Assessment of the Paraspecific Immunoreactivity of Two Antivenoms Evidence the High Compositional and Immunological Conservation Across Lachesis. J Proteomics. 2013 Aug 26;89:112-23. PubMed PMID: 23747394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Snake venomics of Lachesis muta rhombeata and genus-wide antivenomics assessment of the paraspecific immunoreactivity of two antivenoms evidence the high compositional and immunological conservation across Lachesis. AU - Pla,Davinia, AU - Sanz,Libia, AU - Molina-Sánchez,Pedro, AU - Zorita,Virginia, AU - Madrigal,Marvin, AU - Flores-Díaz,Marietta, AU - Alape-Girón,Alberto, AU - Núñez,Vitelbina, AU - Andrés,Vicente, AU - Gutiérrez,José María, AU - Calvete,Juan J, Y1 - 2013/06/04/ PY - 2013/04/15/received PY - 2013/05/08/revised PY - 2013/05/16/accepted PY - 2013/6/11/entrez PY - 2013/6/12/pubmed PY - 2014/3/19/medline KW - Antivenomics KW - Genus Lachesis KW - Lachesis muta rhombeata KW - Mass spectrometry KW - Snake venomics SP - 112 EP - 23 JF - Journal of proteomics JO - J Proteomics VL - 89 N2 - UNLABELLED: We report the proteomic analysis of the Atlantic bushmaster, Lachesis muta rhombeata, from Brazil. Along with previous characterization of the venom proteomes of L. stenophrys (Costa Rica), L. melanocephala (Costa Rica), L. acrochorda (Colombia), and L. muta muta (Bolivia), the present study provides the first overview of the composition and distribution of venom proteins across this wide-ranging genus, and highlights the remarkable similar compositional and pharmacological profiles across Lachesis venoms. The paraspecificity of two antivenoms, produced at Instituto Vital Brazil (Brazil) and Instituto Clodomiro Picado (Costa Rica) using different conspecific taxa in the immunization mixtures, was assessed using genus-wide comparative antivenomics. This study confirms that the proteomic similarity among Lachesis sp. venoms is mirrored in their high immunological conservation across the genus. The clinical and therapeutic consequences of genus-wide venomics and antivenomics investigations of Lachesis venoms are discussed. BIOLOGICAL SIGNIFICANCE: The proteomics characterization of L. m. rhombeata venom completes the overview of Lachesis venom proteomes and confirms the remarkable toxin profile conservation across the five clades of this wide-ranging genus. Genus-wide antivenomics showed that two antivenoms, produced against L. stenophrys or L. m. rhombeata, exhibit paraspecificity towards all other congeneric venoms. Our venomics study shows that, despite the broad geographic distribution of the genus, monospecific antivenoms may achieve clinical coverage for any Lachesis sp. envenoming. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/23747394/Snake_venomics_of_Lachesis_muta_rhombeata_and_genus_wide_antivenomics_assessment_of_the_paraspecific_immunoreactivity_of_two_antivenoms_evidence_the_high_compositional_and_immunological_conservation_across_Lachesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(13)00295-9 DB - PRIME DP - Unbound Medicine ER -