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[Utility of 8-colours multiparameter flow cytometry immunophenotyping of plasma cells for the management of monoclonal gammopathy].
Ann Biol Clin (Paris). 2013 May-Jun; 71(3):313-23.AB

Abstract

Bone marrow flow cytometric analysis is a powerful and rapid tool for evaluating aberrant plasma cell. In this study, we have examined the utility of multiparameter flow cytometry (MFC) in 52 patients with multiple myeloma (MM) and in 45 patients with monoclonal gammopathy with unknown significance (MGUS) into routine evaluation for the management of patients with plasma cell-related disorders. The plasma cells (PC) were identified by their light scatter distribution and reactivity patterns to CD138, CD38, and CD45. The combination of these parameters was helpful for identifying distinct subpopulations of PCs. Moderate to bright expression of CD56, CD20, CD24, CD28, and CD117 was detected in 67%, 26%, 13%, 27%, and 57% of MM cases and in 58%, 20%, 11%, 43% and 44% of MGUS cases, respectively. In MGUS group, the median percentage abnormal PCs/total PCs was 88% with 37 patients out of 45 (82%) with ratio <95%. The median ratio of the MM group was 98.9% and a ratio ≥ 95% was observed in 37 samples out of 44 (84%). In conclusion, MFC immunophenotyping of PCs has obvious clinical relevance in differential diagnosis between MM and others monoclonal gammopathies, identification of high-risk MGUS and smouldering MM, and minimal residual disease monitoring of MM. Our results showed that this tool can be easily applied in haematology laboratories.

Authors+Show Affiliations

Laboratoire d'hématologie, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris AP-HP, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

fre

PubMed ID

23747669

Citation

Gressier, Mélanie, et al. "[Utility of 8-colours Multiparameter Flow Cytometry Immunophenotyping of Plasma Cells for the Management of Monoclonal Gammopathy]." Annales De Biologie Clinique, vol. 71, no. 3, 2013, pp. 313-23.
Gressier M, Chaquin M, Lhermitte L, et al. [Utility of 8-colours multiparameter flow cytometry immunophenotyping of plasma cells for the management of monoclonal gammopathy]. Ann Biol Clin (Paris). 2013;71(3):313-23.
Gressier, M., Chaquin, M., Lhermitte, L., Asnafi, V., Macintyre, E., & Brouzes, C. (2013). [Utility of 8-colours multiparameter flow cytometry immunophenotyping of plasma cells for the management of monoclonal gammopathy]. Annales De Biologie Clinique, 71(3), 313-23. https://doi.org/10.1684/abc.2013.0813
Gressier M, et al. [Utility of 8-colours Multiparameter Flow Cytometry Immunophenotyping of Plasma Cells for the Management of Monoclonal Gammopathy]. Ann Biol Clin (Paris). 2013 May-Jun;71(3):313-23. PubMed PMID: 23747669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Utility of 8-colours multiparameter flow cytometry immunophenotyping of plasma cells for the management of monoclonal gammopathy]. AU - Gressier,Mélanie, AU - Chaquin,Michael, AU - Lhermitte,Ludovic, AU - Asnafi,Vahid, AU - Macintyre,Elizabeth, AU - Brouzes,Chantal, PY - 2013/6/11/entrez PY - 2013/6/12/pubmed PY - 2014/2/13/medline KW - gammopathy of undetermined significance KW - immunophenotyping KW - multiparameter flow cytometry KW - multiple myeloma KW - plasma cells SP - 313 EP - 23 JF - Annales de biologie clinique JO - Ann. Biol. Clin. (Paris) VL - 71 IS - 3 N2 - Bone marrow flow cytometric analysis is a powerful and rapid tool for evaluating aberrant plasma cell. In this study, we have examined the utility of multiparameter flow cytometry (MFC) in 52 patients with multiple myeloma (MM) and in 45 patients with monoclonal gammopathy with unknown significance (MGUS) into routine evaluation for the management of patients with plasma cell-related disorders. The plasma cells (PC) were identified by their light scatter distribution and reactivity patterns to CD138, CD38, and CD45. The combination of these parameters was helpful for identifying distinct subpopulations of PCs. Moderate to bright expression of CD56, CD20, CD24, CD28, and CD117 was detected in 67%, 26%, 13%, 27%, and 57% of MM cases and in 58%, 20%, 11%, 43% and 44% of MGUS cases, respectively. In MGUS group, the median percentage abnormal PCs/total PCs was 88% with 37 patients out of 45 (82%) with ratio <95%. The median ratio of the MM group was 98.9% and a ratio ≥ 95% was observed in 37 samples out of 44 (84%). In conclusion, MFC immunophenotyping of PCs has obvious clinical relevance in differential diagnosis between MM and others monoclonal gammopathies, identification of high-risk MGUS and smouldering MM, and minimal residual disease monitoring of MM. Our results showed that this tool can be easily applied in haematology laboratories. SN - 1950-6112 UR - https://www.unboundmedicine.com/medline/citation/23747669/[Utility_of_8_colours_multiparameter_flow_cytometry_immunophenotyping_of_plasma_cells_for_the_management_of_monoclonal_gammopathy]_ L2 - http://www.john-libbey-eurotext.fr/medline.md?doi=10.1684/abc.2013.0813 DB - PRIME DP - Unbound Medicine ER -