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Use of the Ussing chamber technique to study nutrient transport by epithelial tissues.
Front Biosci (Landmark Ed) 2013; 18:1266-74FB

Abstract

The Ussing chamber provides a physiologically relevant system for measuring the transport of ions, nutrients, and drugs across various epithelial tissues. This article outlines the design, structure, principle, and operation of the Ussing chamber, its application in the field of gastrointestinal barrier function and nutrient transport research, as well as its advantages and limitations. This review serves as a practical guide for investigators who are new to the Ussing chamber and should help researchers better understand this valuable method for measuring the transport of electrolytes, organic nutrients, water, and drugs across the small intestine, placenta, and other epithelial tissues.

Authors+Show Affiliations

Chinese Academy of Sciences, Institute of Subtropical Agriculture, Research Center for Healthy Breeding Livestock and Poultry, Changsha, Hunan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

23747882

Citation

He, Liuqin, et al. "Use of the Ussing Chamber Technique to Study Nutrient Transport By Epithelial Tissues." Frontiers in Bioscience (Landmark Edition), vol. 18, 2013, pp. 1266-74.
He L, Yin Y, Li T, et al. Use of the Ussing chamber technique to study nutrient transport by epithelial tissues. Front Biosci (Landmark Ed). 2013;18:1266-74.
He, L., Yin, Y., Li, T., Huang, R., Xie, M., Wu, Z., & Wu, G. (2013). Use of the Ussing chamber technique to study nutrient transport by epithelial tissues. Frontiers in Bioscience (Landmark Edition), 18, pp. 1266-74.
He L, et al. Use of the Ussing Chamber Technique to Study Nutrient Transport By Epithelial Tissues. Front Biosci (Landmark Ed). 2013 Jun 1;18:1266-74. PubMed PMID: 23747882.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of the Ussing chamber technique to study nutrient transport by epithelial tissues. AU - He,Liuqin, AU - Yin,Yulong, AU - Li,Tiejun, AU - Huang,Rulin, AU - Xie,Mingyong, AU - Wu,Zhenlong, AU - Wu,Guoyao, Y1 - 2013/06/01/ PY - 2013/6/11/entrez PY - 2013/6/12/pubmed PY - 2014/1/25/medline SP - 1266 EP - 74 JF - Frontiers in bioscience (Landmark edition) JO - Front Biosci (Landmark Ed) VL - 18 N2 - The Ussing chamber provides a physiologically relevant system for measuring the transport of ions, nutrients, and drugs across various epithelial tissues. This article outlines the design, structure, principle, and operation of the Ussing chamber, its application in the field of gastrointestinal barrier function and nutrient transport research, as well as its advantages and limitations. This review serves as a practical guide for investigators who are new to the Ussing chamber and should help researchers better understand this valuable method for measuring the transport of electrolytes, organic nutrients, water, and drugs across the small intestine, placenta, and other epithelial tissues. SN - 1093-4715 UR - https://www.unboundmedicine.com/medline/citation/23747882/Use_of_the_Ussing_chamber_technique_to_study_nutrient_transport_by_epithelial_tissues_ L2 - https://www.bioscience.org/2013/v18/af/4178/fulltext.htm DB - PRIME DP - Unbound Medicine ER -