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Oxidoreductases that act as conditional virulence suppressors in Salmonella enterica serovar Typhimurium.
PLoS One. 2013; 8(6):e64948.Plos

Abstract

In Salmonella enterica serovar Typhimurium, oxidoreductases of the thioredoxin superfamily contribute to bacterial invasiveness, intracellular replication and to the virulence in BALB/c mice as well as in the soil nematode Caenorhabditis elegans. The scsABCD gene cluster, present in many but not all enteric bacteria, codes for four putative oxidoreductases of the thioredoxin superfamily. Here we have analyzed the potential role of the scs genes in oxidative stress tolerance and virulence in S. Typhimurium. An scsABCD deletion mutant showed moderate sensitization to the redox-active transition metal ion copper and increased protein carbonylation upon exposure to hydrogen peroxide. Still, the scsABCD mutant was not significantly affected for invasiveness or intracellular replication in respectively cultured epithelial or macrophage-like cells. However, we noted a significant copper chloride sensitivity of SPI1 T3SS mediated invasiveness that strongly depended on the presence of the scs genes. The scsABCD deletion mutant was not attenuated in animal infection models. In contrast, the mutant showed a moderate increase in its competitive index upon intraperitoneal challenge and enhanced invasiveness in small intestinal ileal loops of BALB/c mice. Moreover, deletion of the scsABCD genes restored the invasiveness of a trxA mutant in epithelial cells and its virulence in C. elegans. Our findings thus demonstrate that the scs gene cluster conditionally affects virulence and underscore the complex interactions between oxidoreductases of the thioredoxin superfamily in maintaining host adaptation of S. Typhimurium.

Authors+Show Affiliations

Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23750221

Citation

Anwar, Naeem, et al. "Oxidoreductases That Act as Conditional Virulence Suppressors in Salmonella Enterica Serovar Typhimurium." PloS One, vol. 8, no. 6, 2013, pp. e64948.
Anwar N, Sem XH, Rhen M. Oxidoreductases that act as conditional virulence suppressors in Salmonella enterica serovar Typhimurium. PLoS One. 2013;8(6):e64948.
Anwar, N., Sem, X. H., & Rhen, M. (2013). Oxidoreductases that act as conditional virulence suppressors in Salmonella enterica serovar Typhimurium. PloS One, 8(6), e64948. https://doi.org/10.1371/journal.pone.0064948
Anwar N, Sem XH, Rhen M. Oxidoreductases That Act as Conditional Virulence Suppressors in Salmonella Enterica Serovar Typhimurium. PLoS One. 2013;8(6):e64948. PubMed PMID: 23750221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidoreductases that act as conditional virulence suppressors in Salmonella enterica serovar Typhimurium. AU - Anwar,Naeem, AU - Sem,Xiao Hui, AU - Rhen,Mikael, Y1 - 2013/06/04/ PY - 2012/12/17/received PY - 2013/04/19/accepted PY - 2013/6/11/entrez PY - 2013/6/12/pubmed PY - 2014/9/30/medline SP - e64948 EP - e64948 JF - PloS one JO - PLoS One VL - 8 IS - 6 N2 - In Salmonella enterica serovar Typhimurium, oxidoreductases of the thioredoxin superfamily contribute to bacterial invasiveness, intracellular replication and to the virulence in BALB/c mice as well as in the soil nematode Caenorhabditis elegans. The scsABCD gene cluster, present in many but not all enteric bacteria, codes for four putative oxidoreductases of the thioredoxin superfamily. Here we have analyzed the potential role of the scs genes in oxidative stress tolerance and virulence in S. Typhimurium. An scsABCD deletion mutant showed moderate sensitization to the redox-active transition metal ion copper and increased protein carbonylation upon exposure to hydrogen peroxide. Still, the scsABCD mutant was not significantly affected for invasiveness or intracellular replication in respectively cultured epithelial or macrophage-like cells. However, we noted a significant copper chloride sensitivity of SPI1 T3SS mediated invasiveness that strongly depended on the presence of the scs genes. The scsABCD deletion mutant was not attenuated in animal infection models. In contrast, the mutant showed a moderate increase in its competitive index upon intraperitoneal challenge and enhanced invasiveness in small intestinal ileal loops of BALB/c mice. Moreover, deletion of the scsABCD genes restored the invasiveness of a trxA mutant in epithelial cells and its virulence in C. elegans. Our findings thus demonstrate that the scs gene cluster conditionally affects virulence and underscore the complex interactions between oxidoreductases of the thioredoxin superfamily in maintaining host adaptation of S. Typhimurium. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23750221/Oxidoreductases_that_act_as_conditional_virulence_suppressors_in_Salmonella_enterica_serovar_Typhimurium_ L2 - https://dx.plos.org/10.1371/journal.pone.0064948 DB - PRIME DP - Unbound Medicine ER -