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Blueberry treatment attenuates D-galactose-induced oxidative stress and tissue damage in rat liver.
Geriatr Gerontol Int 2014; 14(2):490-7GG

Abstract

AIM

d-galactose (GAL) causes aging-related changes and oxidative stress in the organism. We investigated the effect of whole fresh blueberry (BB; Vaccinium corymbosum L.) treatment on oxidative stress in age-related liver injury model.

METHODS

Rats received GAL (300 mg/kg, s.c.; 5 days per week) alone or together with 5% (BB1) and 10% (BB2) BB-containing chow for 2 months. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, and hepatic malondialdehyde (MDA), protein carbonyl (PC) and glutathione (GSH) levels, and CuZn-superoxide dismutase (SOD1), glutathione peroxidase (GPx1) and glutathione transferase (GST) activities together with mRNA expressions of SOD1, GPx1, MnSOD (SOD2) and phospholipid hydroperoxide glutathione peroxidase (GPx4) were determined in GAL-treated rats.

RESULTS

MDA and PC levels increased, but GSH levels, SOD1 and GPx1 activities decreased together with histopathological structural damage in the liver in GAL-treated rats. There was no change in hepatic mRNA expressions of SOD2 and GPx1, but SOD1 and GPx4 expressions decreased. BB1 and BB2 caused significant decreases in serum ALT and AST activities together with the amelioration in histopathological findings in GAL-treated rats. Both BB1 and BB2 reduced MDA and PC levels, and elevated GSH levels, and SOD1 and GPx1 activities. However, hepatic mRNA expressions of SOD1, SOD2, GPx1 and GPx4 remained unchanged in GAL-treated rats.

CONCLUSIONS

These results show that BB restored liver pro-oxidant status together with histopathological amelioration by acting as an anti-oxidant (radical scavenger) itself without affecting mRNA expressions of anti-oxidant enzymes in GAL-treated rats.

Authors+Show Affiliations

Department of Biochemistry, Yeditepe University Medical Faculty, Istanbul, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23750655

Citation

Çoban, Jale, et al. "Blueberry Treatment Attenuates D-galactose-induced Oxidative Stress and Tissue Damage in Rat Liver." Geriatrics & Gerontology International, vol. 14, no. 2, 2014, pp. 490-7.
Çoban J, Betül-Kalaz E, Küçükgergin C, et al. Blueberry treatment attenuates D-galactose-induced oxidative stress and tissue damage in rat liver. Geriatr Gerontol Int. 2014;14(2):490-7.
Çoban, J., Betül-Kalaz, E., Küçükgergin, C., Aydin, A. F., Doğan-Ekici, I., Doğru-Abbasoğlu, S., & Uysal, M. (2014). Blueberry treatment attenuates D-galactose-induced oxidative stress and tissue damage in rat liver. Geriatrics & Gerontology International, 14(2), pp. 490-7. doi:10.1111/ggi.12096.
Çoban J, et al. Blueberry Treatment Attenuates D-galactose-induced Oxidative Stress and Tissue Damage in Rat Liver. Geriatr Gerontol Int. 2014;14(2):490-7. PubMed PMID: 23750655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blueberry treatment attenuates D-galactose-induced oxidative stress and tissue damage in rat liver. AU - Çoban,Jale, AU - Betül-Kalaz,Esra, AU - Küçükgergin,Canan, AU - Aydin,A Fatih, AU - Doğan-Ekici,Işin, AU - Doğru-Abbasoğlu,Semra, AU - Uysal,Müjdat, Y1 - 2013/06/10/ PY - 2013/04/16/accepted PY - 2013/6/12/entrez PY - 2013/6/12/pubmed PY - 2014/12/15/medline KW - aging KW - blueberry KW - galactose KW - liver KW - oxidative stress SP - 490 EP - 7 JF - Geriatrics & gerontology international JO - Geriatr Gerontol Int VL - 14 IS - 2 N2 - AIM: d-galactose (GAL) causes aging-related changes and oxidative stress in the organism. We investigated the effect of whole fresh blueberry (BB; Vaccinium corymbosum L.) treatment on oxidative stress in age-related liver injury model. METHODS: Rats received GAL (300 mg/kg, s.c.; 5 days per week) alone or together with 5% (BB1) and 10% (BB2) BB-containing chow for 2 months. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, and hepatic malondialdehyde (MDA), protein carbonyl (PC) and glutathione (GSH) levels, and CuZn-superoxide dismutase (SOD1), glutathione peroxidase (GPx1) and glutathione transferase (GST) activities together with mRNA expressions of SOD1, GPx1, MnSOD (SOD2) and phospholipid hydroperoxide glutathione peroxidase (GPx4) were determined in GAL-treated rats. RESULTS: MDA and PC levels increased, but GSH levels, SOD1 and GPx1 activities decreased together with histopathological structural damage in the liver in GAL-treated rats. There was no change in hepatic mRNA expressions of SOD2 and GPx1, but SOD1 and GPx4 expressions decreased. BB1 and BB2 caused significant decreases in serum ALT and AST activities together with the amelioration in histopathological findings in GAL-treated rats. Both BB1 and BB2 reduced MDA and PC levels, and elevated GSH levels, and SOD1 and GPx1 activities. However, hepatic mRNA expressions of SOD1, SOD2, GPx1 and GPx4 remained unchanged in GAL-treated rats. CONCLUSIONS: These results show that BB restored liver pro-oxidant status together with histopathological amelioration by acting as an anti-oxidant (radical scavenger) itself without affecting mRNA expressions of anti-oxidant enzymes in GAL-treated rats. SN - 1447-0594 UR - https://www.unboundmedicine.com/medline/citation/23750655/Blueberry_treatment_attenuates_D_galactose_induced_oxidative_stress_and_tissue_damage_in_rat_liver_ L2 - https://doi.org/10.1111/ggi.12096 DB - PRIME DP - Unbound Medicine ER -