Tags

Type your tag names separated by a space and hit enter

Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer.
Oncol Rep 2013; 30(2):897-903OR

Abstract

Disrupted apoptosis not only confers a survival advantage to cancer cells but also causes resistance to chemotherapies. Therefore, inducing cell apoptosis has become a promising strategy for anticancer treatment. Patrinia scabiosaefolia (PS) has long been used to clinically treat various types of malignancies including colorectal cancer (CRC). However, the precise mechanism of its tumoricidal activity remains largely unclear. Using a CRC mouse xenograft model and a human colon carcinoma cell line, HT-29, in the present study, we evaluated the antitumor activities of an ethanol extract of Patrinia scabiosaefolia (EEPS), and investigated the underlying molecular mechanisms. We found that EEPS inhibited CRC growth both in vivo and in vitro, without apparent adverse side-effects. Moreover, EEPS treatment promoted apoptosis in CRC tumor tissues and in HT-29 cells, suggesting that the inhibitory effect of EEPS on tumor growth was due to its pro-apoptotic activity. Furthermore, EEPS treatment inhibited the expression of anti-apoptotic Bcl-2 but enhanced pro-apoptotic Bax expression at both transcriptional and translational levels. Finally, EEPS induced the loss of mitochondrial membrane potential and activation of caspase-9 and -3 in HT-29 cells. Taken together, data in this study suggest that induction of cancer cell apoptosis via the mitochondrial-dependent pathway may be one of the mechanisms whereby PS exerts anticancer activity.

Authors+Show Affiliations

Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350122, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23754194

Citation

Liu, Liya, et al. "Patrinia Scabiosaefolia Induces Mitochondrial-dependent Apoptosis in a Mouse Model of Colorectal Cancer." Oncology Reports, vol. 30, no. 2, 2013, pp. 897-903.
Liu L, Shen A, Chen Y, et al. Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer. Oncol Rep. 2013;30(2):897-903.
Liu, L., Shen, A., Chen, Y., Wei, L., Lin, J., Sferra, T. J., ... Peng, J. (2013). Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer. Oncology Reports, 30(2), pp. 897-903. doi:10.3892/or.2013.2528.
Liu L, et al. Patrinia Scabiosaefolia Induces Mitochondrial-dependent Apoptosis in a Mouse Model of Colorectal Cancer. Oncol Rep. 2013;30(2):897-903. PubMed PMID: 23754194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer. AU - Liu,Liya, AU - Shen,Aling, AU - Chen,Youqin, AU - Wei,Lihui, AU - Lin,Jiumao, AU - Sferra,Thomas J, AU - Hong,Zhenfeng, AU - Peng,Jun, Y1 - 2013/06/07/ PY - 2013/03/22/received PY - 2013/05/17/accepted PY - 2013/6/12/entrez PY - 2013/6/12/pubmed PY - 2014/2/28/medline SP - 897 EP - 903 JF - Oncology reports JO - Oncol. Rep. VL - 30 IS - 2 N2 - Disrupted apoptosis not only confers a survival advantage to cancer cells but also causes resistance to chemotherapies. Therefore, inducing cell apoptosis has become a promising strategy for anticancer treatment. Patrinia scabiosaefolia (PS) has long been used to clinically treat various types of malignancies including colorectal cancer (CRC). However, the precise mechanism of its tumoricidal activity remains largely unclear. Using a CRC mouse xenograft model and a human colon carcinoma cell line, HT-29, in the present study, we evaluated the antitumor activities of an ethanol extract of Patrinia scabiosaefolia (EEPS), and investigated the underlying molecular mechanisms. We found that EEPS inhibited CRC growth both in vivo and in vitro, without apparent adverse side-effects. Moreover, EEPS treatment promoted apoptosis in CRC tumor tissues and in HT-29 cells, suggesting that the inhibitory effect of EEPS on tumor growth was due to its pro-apoptotic activity. Furthermore, EEPS treatment inhibited the expression of anti-apoptotic Bcl-2 but enhanced pro-apoptotic Bax expression at both transcriptional and translational levels. Finally, EEPS induced the loss of mitochondrial membrane potential and activation of caspase-9 and -3 in HT-29 cells. Taken together, data in this study suggest that induction of cancer cell apoptosis via the mitochondrial-dependent pathway may be one of the mechanisms whereby PS exerts anticancer activity. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/23754194/Patrinia_scabiosaefolia_induces_mitochondrial_dependent_apoptosis_in_a_mouse_model_of_colorectal_cancer_ L2 - http://www.spandidos-publications.com/or/30/2/897 DB - PRIME DP - Unbound Medicine ER -