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A leafhopper-transmissible DNA virus with novel evolutionary lineage in the family geminiviridae implicated in grapevine redleaf disease by next-generation sequencing.
PLoS One. 2013; 8(6):e64194.Plos

Abstract

A graft-transmissible disease displaying red veins, red blotches and total reddening of leaves in red-berried wine grape (Vitis vinifera L.) cultivars was observed in commercial vineyards. Next-generation sequencing technology was used to identify etiological agent(s) associated with this emerging disease, designated as grapevine redleaf disease (GRD). High quality RNA extracted from leaves of grape cultivars Merlot and Cabernet Franc with and without GRD symptoms was used to prepare cDNA libraries. Assembly of highly informative sequence reads generated from Illumina sequencing of cDNA libraries, followed by bioinformatic analyses of sequence contigs resulted in specific identification of taxonomically disparate viruses and viroids in samples with and without GRD symptoms. A single-stranded DNA virus, tentatively named Grapevine redleaf-associated virus (GRLaV), and Grapevine fanleaf virus were detected only in grapevines showing GRD symptoms. In contrast, Grapevine rupestris stem pitting-associated virus, Hop stunt viroid, Grapevine yellow speckle viroid 1, Citrus exocortis viroid and Citrus exocortis Yucatan viroid were present in both symptomatic and non-symptomatic grapevines. GRLaV was transmitted by the Virginia creeper leafhopper (Erythroneura ziczac Walsh) from grapevine-to-grapevine under greenhouse conditions. Molecular and phylogenetic analyses indicated that GRLaV, almost identical to recently reported Grapevine Cabernet Franc-associated virus from New York and Grapevine red blotch-associated virus from California, represents an evolutionarily distinct lineage in the family Geminiviridae with genome characteristics distinct from other leafhopper-transmitted geminiviruses. GRD significantly reduced fruit yield and affected berry quality parameters demonstrating negative impacts of the disease. Higher quantities of carbohydrates were present in symptomatic leaves suggesting their possible role in the expression of redleaf symptoms.

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Authors+Show Affiliations

Poojari S
Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser, Washington, United States of America.
Alabi OJ
No affiliation info available
Fofanov VY
No affiliation info available
Naidu RA
No affiliation info available

MeSH

AnimalsCrops, AgriculturalEvolution, MolecularFruitGeminiviridaeGene LibraryGenes, ViralHemipteraHigh-Throughput Nucleotide SequencingInsect VectorsMolecular TypingPhylogenyPlant DiseasesPlant LeavesSequence Analysis, DNAVitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23755117

Citation

Poojari, Sudarsana, et al. "A Leafhopper-transmissible DNA Virus With Novel Evolutionary Lineage in the Family Geminiviridae Implicated in Grapevine Redleaf Disease By Next-generation Sequencing." PloS One, vol. 8, no. 6, 2013, pp. e64194.
Poojari S, Alabi OJ, Fofanov VY, et al. A leafhopper-transmissible DNA virus with novel evolutionary lineage in the family geminiviridae implicated in grapevine redleaf disease by next-generation sequencing. PLoS One. 2013;8(6):e64194.
Poojari, S., Alabi, O. J., Fofanov, V. Y., & Naidu, R. A. (2013). A leafhopper-transmissible DNA virus with novel evolutionary lineage in the family geminiviridae implicated in grapevine redleaf disease by next-generation sequencing. PloS One, 8(6), e64194. https://doi.org/10.1371/journal.pone.0064194
Poojari S, et al. A Leafhopper-transmissible DNA Virus With Novel Evolutionary Lineage in the Family Geminiviridae Implicated in Grapevine Redleaf Disease By Next-generation Sequencing. PLoS One. 2013;8(6):e64194. PubMed PMID: 23755117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A leafhopper-transmissible DNA virus with novel evolutionary lineage in the family geminiviridae implicated in grapevine redleaf disease by next-generation sequencing. AU - Poojari,Sudarsana, AU - Alabi,Olufemi J, AU - Fofanov,Viacheslav Y, AU - Naidu,Rayapati A, Y1 - 2013/06/05/ PY - 2013/02/13/received PY - 2013/04/10/accepted PY - 2013/6/12/entrez PY - 2013/6/12/pubmed PY - 2014/1/15/medline SP - e64194 EP - e64194 JF - PloS one JO - PLoS One VL - 8 IS - 6 N2 - A graft-transmissible disease displaying red veins, red blotches and total reddening of leaves in red-berried wine grape (Vitis vinifera L.) cultivars was observed in commercial vineyards. Next-generation sequencing technology was used to identify etiological agent(s) associated with this emerging disease, designated as grapevine redleaf disease (GRD). High quality RNA extracted from leaves of grape cultivars Merlot and Cabernet Franc with and without GRD symptoms was used to prepare cDNA libraries. Assembly of highly informative sequence reads generated from Illumina sequencing of cDNA libraries, followed by bioinformatic analyses of sequence contigs resulted in specific identification of taxonomically disparate viruses and viroids in samples with and without GRD symptoms. A single-stranded DNA virus, tentatively named Grapevine redleaf-associated virus (GRLaV), and Grapevine fanleaf virus were detected only in grapevines showing GRD symptoms. In contrast, Grapevine rupestris stem pitting-associated virus, Hop stunt viroid, Grapevine yellow speckle viroid 1, Citrus exocortis viroid and Citrus exocortis Yucatan viroid were present in both symptomatic and non-symptomatic grapevines. GRLaV was transmitted by the Virginia creeper leafhopper (Erythroneura ziczac Walsh) from grapevine-to-grapevine under greenhouse conditions. Molecular and phylogenetic analyses indicated that GRLaV, almost identical to recently reported Grapevine Cabernet Franc-associated virus from New York and Grapevine red blotch-associated virus from California, represents an evolutionarily distinct lineage in the family Geminiviridae with genome characteristics distinct from other leafhopper-transmitted geminiviruses. GRD significantly reduced fruit yield and affected berry quality parameters demonstrating negative impacts of the disease. Higher quantities of carbohydrates were present in symptomatic leaves suggesting their possible role in the expression of redleaf symptoms. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23755117/A_leafhopper_transmissible_DNA_virus_with_novel_evolutionary_lineage_in_the_family_geminiviridae_implicated_in_grapevine_redleaf_disease_by_next_generation_sequencing_ L2 - https://dx.plos.org/10.1371/journal.pone.0064194 DB - PRIME DP - Unbound Medicine ER -