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[Human rhinovirus with different genotypes in children with acute respiratory tract infections in Beijing].
Bing Du Xue Bao. 2013 Mar; 29(2):97-105.BD

Abstract

To understand the infections and molecular biological characteristics of different human rhinovirus (HRV) genotypes -A, B, C, especially C in children with acute respiratory tract infections (ARI) in Beijing. Seven hundreds and three respiratory tract specimens were collected from children with ARI during Jan. 2011 to Dec. 2011. Semi-nested PCR was developed for detecting HRVs. Gene fragment of VP4/VP2 capsid protein amplified from HRV positive specimens was sequenced and analyzed by software DNAStar, the phylogenetic tree was then constructed by MEGA 5. 05. Among these 703 specimens tested, 54 (7.7%, 54/703) were HRV positive, including 25 (46.3%, 25/54) positive for HRV-A, 8 (14. 8%, 8/54) for HRV-B, 21 (38. 9%, 21/54) for HRV-C determined by sequence analysis. Most of these children (94. 4%00, 51/54) infected with HRVs were younger than 5 years old, and the highest positive rate was shown in group younger than 1 year (11. 4%). These patients positive for HRVs were diagnosed as bronchiolitis (23.1%), asthma (20.0%), pneumonia (1.0%), bronchitis (4.4%) and upper respiratory tract infections (4. 1%). Sequence analysis of VP4/VP2 gene fragment revealed that 70. 0% to 100. 0% nucleotide identity was shown among the sequences within the same HRV genotype, and 55. 5% to 65. 8% nucleotide identity among the sequences from different HRV genotypes. In conclusion, HRVs, especially HRV-C, are important pathogens for children with ARI in Beijing. The prevalence of HRV-C is similar to that of HRV-A, higher than that of HRV-B. High sequence variation among different HRV genotypes was indicated in this study.

Authors+Show Affiliations

Laboratory of Virology, Capital Institute of Pediatrics, Beijing 100020, China. mdjsongminghui@sina.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23757837

Citation

Song, Ming-Hui, et al. "[Human Rhinovirus With Different Genotypes in Children With Acute Respiratory Tract Infections in Beijing]." Bing Du Xue Bao = Chinese Journal of Virology, vol. 29, no. 2, 2013, pp. 97-105.
Song MH, Zhao LQ, Qian Y, et al. [Human rhinovirus with different genotypes in children with acute respiratory tract infections in Beijing]. Bing Du Xue Bao. 2013;29(2):97-105.
Song, M. H., Zhao, L. Q., Qian, Y., Zhu, R. N., Deng, J., Wang, F., Sun, Y., & Tian, R. (2013). [Human rhinovirus with different genotypes in children with acute respiratory tract infections in Beijing]. Bing Du Xue Bao = Chinese Journal of Virology, 29(2), 97-105.
Song MH, et al. [Human Rhinovirus With Different Genotypes in Children With Acute Respiratory Tract Infections in Beijing]. Bing Du Xue Bao. 2013;29(2):97-105. PubMed PMID: 23757837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Human rhinovirus with different genotypes in children with acute respiratory tract infections in Beijing]. AU - Song,Ming-Hui, AU - Zhao,Lin-Qing, AU - Qian,Yuan, AU - Zhu,Ru-Nan, AU - Deng,Jie, AU - Wang,Fang, AU - Sun,Yu, AU - Tian,Run, PY - 2013/6/14/entrez PY - 2013/6/14/pubmed PY - 2013/7/17/medline SP - 97 EP - 105 JF - Bing du xue bao = Chinese journal of virology JO - Bing Du Xue Bao VL - 29 IS - 2 N2 - To understand the infections and molecular biological characteristics of different human rhinovirus (HRV) genotypes -A, B, C, especially C in children with acute respiratory tract infections (ARI) in Beijing. Seven hundreds and three respiratory tract specimens were collected from children with ARI during Jan. 2011 to Dec. 2011. Semi-nested PCR was developed for detecting HRVs. Gene fragment of VP4/VP2 capsid protein amplified from HRV positive specimens was sequenced and analyzed by software DNAStar, the phylogenetic tree was then constructed by MEGA 5. 05. Among these 703 specimens tested, 54 (7.7%, 54/703) were HRV positive, including 25 (46.3%, 25/54) positive for HRV-A, 8 (14. 8%, 8/54) for HRV-B, 21 (38. 9%, 21/54) for HRV-C determined by sequence analysis. Most of these children (94. 4%00, 51/54) infected with HRVs were younger than 5 years old, and the highest positive rate was shown in group younger than 1 year (11. 4%). These patients positive for HRVs were diagnosed as bronchiolitis (23.1%), asthma (20.0%), pneumonia (1.0%), bronchitis (4.4%) and upper respiratory tract infections (4. 1%). Sequence analysis of VP4/VP2 gene fragment revealed that 70. 0% to 100. 0% nucleotide identity was shown among the sequences within the same HRV genotype, and 55. 5% to 65. 8% nucleotide identity among the sequences from different HRV genotypes. In conclusion, HRVs, especially HRV-C, are important pathogens for children with ARI in Beijing. The prevalence of HRV-C is similar to that of HRV-A, higher than that of HRV-B. High sequence variation among different HRV genotypes was indicated in this study. SN - 1000-8721 UR - https://www.unboundmedicine.com/medline/citation/23757837/[Human_rhinovirus_with_different_genotypes_in_children_with_acute_respiratory_tract_infections_in_Beijing]_ L2 - https://antibodies.cancer.gov/detail/CPTC-PTEN-1 DB - PRIME DP - Unbound Medicine ER -