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Consistency of the key genotypes of Orientia tsutsugamushi in scrub typhus patients, rodents, and chiggers from a new endemic focus of northern China.
Cell Biochem Biophys. 2013; 67(3):1461-6.CB

Abstract

Scrub typhus is one of the most common infectious diseases of rural south and southeastern Asia and the western Pacific. It emerged in Shandong Province in northern China from autumn to winter of 1986. Since then, the "autumn-winter type scrub typhus" has been found in many areas of northern China. However, the principle genotypes of Orientia tsutsugamushi still remain unknown. This study was undertaken to identify the genotypes of O. tsutsugamushi obtained from scrub typhus patients, chigger mites and rodents from the focal point of the problem in Shandong Province. Forty-four isolates from patients, rodents, and chiggers, 47 blood clots from patients during the acute phase, 10 eschars from patients during the convalescence phase and 16 pools of larval chiggers were tested for the scrub typhus antigen 56-kD protein (Sta56) gene by nested PCR methodology and additional sequence analysis including DNA sequence alignment and phylogenetic analysis. Based on nested PCR, ninety-five initial PCR-positive samples produced amplicons using Kawasaki strain-specific primers, while the other two (the FXS4 and LHGM2 strains) produced amplicons using Karp strain-specific primers. The partial Sta56 gene sequence analysis indicated that the sequence homologies of 3 selected isolates (the B16, FXS2, and XDM2 strains) and 7 eschars out of the 95 samples, which were nested PCR-positive using Kawasaki strain-specific primers, were 94-98% to that of Kawasaki strain. The sequence homology of the FXS4 and LHGM2 strains to that of the Karp strain was respectively 83 and 96%. These findings implied that the key genotypes of O. tsutsugamushi in patients, rodents, and chiggers in Shandong Province were identical and similar to Kawasaki strains.

Authors+Show Affiliations

Department of Nosocomial Infection Management and Disease Control, Institute of Hospital Management, Chinese People's Liberation Army General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, People's Republic of China, Yunxiliu6511@yahoo.com.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23760611

Citation

Liu, Yun-Xi, et al. "Consistency of the Key Genotypes of Orientia Tsutsugamushi in Scrub Typhus Patients, Rodents, and Chiggers From a New Endemic Focus of Northern China." Cell Biochemistry and Biophysics, vol. 67, no. 3, 2013, pp. 1461-6.
Liu YX, Jia N, Xing YB, et al. Consistency of the key genotypes of Orientia tsutsugamushi in scrub typhus patients, rodents, and chiggers from a new endemic focus of northern China. Cell Biochem Biophys. 2013;67(3):1461-6.
Liu, Y. X., Jia, N., Xing, Y. B., Suo, J. J., Du, M. M., Jia, N., Gao, Y., Xie, L. J., Liu, B. W., & Ren, S. W. (2013). Consistency of the key genotypes of Orientia tsutsugamushi in scrub typhus patients, rodents, and chiggers from a new endemic focus of northern China. Cell Biochemistry and Biophysics, 67(3), 1461-6. https://doi.org/10.1007/s12013-013-9646-0
Liu YX, et al. Consistency of the Key Genotypes of Orientia Tsutsugamushi in Scrub Typhus Patients, Rodents, and Chiggers From a New Endemic Focus of Northern China. Cell Biochem Biophys. 2013;67(3):1461-6. PubMed PMID: 23760611.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Consistency of the key genotypes of Orientia tsutsugamushi in scrub typhus patients, rodents, and chiggers from a new endemic focus of northern China. AU - Liu,Yun-Xi, AU - Jia,Na, AU - Xing,Yu-Bin, AU - Suo,Ji-Jiang, AU - Du,Ming-Mei, AU - Jia,Ning, AU - Gao,Yan, AU - Xie,Li-Jun, AU - Liu,Bo-Wei, AU - Ren,Shi-Wang, PY - 2013/6/14/entrez PY - 2013/6/14/pubmed PY - 2014/7/16/medline SP - 1461 EP - 6 JF - Cell biochemistry and biophysics JO - Cell Biochem Biophys VL - 67 IS - 3 N2 - Scrub typhus is one of the most common infectious diseases of rural south and southeastern Asia and the western Pacific. It emerged in Shandong Province in northern China from autumn to winter of 1986. Since then, the "autumn-winter type scrub typhus" has been found in many areas of northern China. However, the principle genotypes of Orientia tsutsugamushi still remain unknown. This study was undertaken to identify the genotypes of O. tsutsugamushi obtained from scrub typhus patients, chigger mites and rodents from the focal point of the problem in Shandong Province. Forty-four isolates from patients, rodents, and chiggers, 47 blood clots from patients during the acute phase, 10 eschars from patients during the convalescence phase and 16 pools of larval chiggers were tested for the scrub typhus antigen 56-kD protein (Sta56) gene by nested PCR methodology and additional sequence analysis including DNA sequence alignment and phylogenetic analysis. Based on nested PCR, ninety-five initial PCR-positive samples produced amplicons using Kawasaki strain-specific primers, while the other two (the FXS4 and LHGM2 strains) produced amplicons using Karp strain-specific primers. The partial Sta56 gene sequence analysis indicated that the sequence homologies of 3 selected isolates (the B16, FXS2, and XDM2 strains) and 7 eschars out of the 95 samples, which were nested PCR-positive using Kawasaki strain-specific primers, were 94-98% to that of Kawasaki strain. The sequence homology of the FXS4 and LHGM2 strains to that of the Karp strain was respectively 83 and 96%. These findings implied that the key genotypes of O. tsutsugamushi in patients, rodents, and chiggers in Shandong Province were identical and similar to Kawasaki strains. SN - 1559-0283 UR - https://www.unboundmedicine.com/medline/citation/23760611/Consistency_of_the_key_genotypes_of_Orientia_tsutsugamushi_in_scrub_typhus_patients_rodents_and_chiggers_from_a_new_endemic_focus_of_northern_China_ L2 - https://dx.doi.org/10.1007/s12013-013-9646-0 DB - PRIME DP - Unbound Medicine ER -