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Selective extraction of lamivudine in human serum and urine using molecularly imprinted polymer technique.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jul 15; 931:50-5.JC

Abstract

In this work, a novel technique is described for determination of lamivudine in biological fluids by molecularly imprinted polymers (MIPs) as the sample clean-up method joint with high performance liquid chromatography (HPLC). MIPs were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as crosslinker, acetonitrile and tetrahydrofuran as porogen and lamivudine as the template molecule. The new imprinted polymer was used as a molecular sorbent for the separation of lamivudine from human serum and urine. Molecular recognition properties, binding capacity and selectivity of the MIPs were evaluated and the results showed that the obtained MIPs have a high affinity for lamivudine in aqueous medium. HPLC analyses showed that the extraction of lamivudine from serum and urine by MIPs had a linear calibration curve in the range of 60-700μg/L with excellent precisions of 2.73% for serum and 2.60% for urine. The limit of detection and quantization of lamivudine was 19.34 and 58.6μg/L in serum and 7.95 and 24.05μg/L in urine respectively. MIP extraction provided about 10 fold LOQ improvement in serum and 5 fold LOQ improvement in urine samples. The recoveries of lamivudine in serum and urine samples were found to be 84.2-93.5% and 82.5-90.8% respectively. Due to the high precision and accuracy, this method may be the UV-HPLC choice with MIP extraction for bioequivalence analysis of lamivudine in serum and urine.

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Authors+Show Affiliations

Shekarchi M
Food and Drug Laboratory Research Center, Food and Drug Organization, MOHME, Tehran, Iran.
Pourfarzib M
No affiliation info available
Akbari-Adergani B
No affiliation info available
Mehramizi A
No affiliation info available
Javanbakht M
No affiliation info available
Dinarvand R
No affiliation info available

MeSH

Acetic AcidAdsorptionChromatography, High Pressure LiquidEthylene GlycolsHumansHydrogen-Ion ConcentrationLamivudineLimit of DetectionMethacrylatesMethanolMolecular ImprintingReproducibility of Results

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23764731

Citation

Shekarchi, Maryam, et al. "Selective Extraction of Lamivudine in Human Serum and Urine Using Molecularly Imprinted Polymer Technique." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 931, 2013, pp. 50-5.
Shekarchi M, Pourfarzib M, Akbari-Adergani B, et al. Selective extraction of lamivudine in human serum and urine using molecularly imprinted polymer technique. J Chromatogr B Analyt Technol Biomed Life Sci. 2013;931:50-5.
Shekarchi, M., Pourfarzib, M., Akbari-Adergani, B., Mehramizi, A., Javanbakht, M., & Dinarvand, R. (2013). Selective extraction of lamivudine in human serum and urine using molecularly imprinted polymer technique. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 931, 50-5. https://doi.org/10.1016/j.jchromb.2013.04.041
Shekarchi M, et al. Selective Extraction of Lamivudine in Human Serum and Urine Using Molecularly Imprinted Polymer Technique. J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jul 15;931:50-5. PubMed PMID: 23764731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective extraction of lamivudine in human serum and urine using molecularly imprinted polymer technique. AU - Shekarchi,Maryam, AU - Pourfarzib,Mojgan, AU - Akbari-Adergani,Behrouz, AU - Mehramizi,Ali, AU - Javanbakht,Mehran, AU - Dinarvand,Rassoul, Y1 - 2013/05/21/ PY - 2013/01/27/received PY - 2013/04/24/revised PY - 2013/04/30/accepted PY - 2013/6/15/entrez PY - 2013/6/15/pubmed PY - 2014/1/15/medline SP - 50 EP - 5 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 931 N2 - In this work, a novel technique is described for determination of lamivudine in biological fluids by molecularly imprinted polymers (MIPs) as the sample clean-up method joint with high performance liquid chromatography (HPLC). MIPs were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as crosslinker, acetonitrile and tetrahydrofuran as porogen and lamivudine as the template molecule. The new imprinted polymer was used as a molecular sorbent for the separation of lamivudine from human serum and urine. Molecular recognition properties, binding capacity and selectivity of the MIPs were evaluated and the results showed that the obtained MIPs have a high affinity for lamivudine in aqueous medium. HPLC analyses showed that the extraction of lamivudine from serum and urine by MIPs had a linear calibration curve in the range of 60-700μg/L with excellent precisions of 2.73% for serum and 2.60% for urine. The limit of detection and quantization of lamivudine was 19.34 and 58.6μg/L in serum and 7.95 and 24.05μg/L in urine respectively. MIP extraction provided about 10 fold LOQ improvement in serum and 5 fold LOQ improvement in urine samples. The recoveries of lamivudine in serum and urine samples were found to be 84.2-93.5% and 82.5-90.8% respectively. Due to the high precision and accuracy, this method may be the UV-HPLC choice with MIP extraction for bioequivalence analysis of lamivudine in serum and urine. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/23764731/Selective_extraction_of_lamivudine_in_human_serum_and_urine_using_molecularly_imprinted_polymer_technique_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(13)00265-1 DB - PRIME DP - Unbound Medicine ER -
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