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Comparative biological study of roots, stems, leaves, and seeds of Angelica shikokiana Makino.
J Ethnopharmacol. 2013 Jul 30; 148(3):980-7.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Angelica shikokiana has been used as a health food for its anticancer, anti-inflammatory, antibacterial, antiallergic, and blood vessel dilating effects in Japan. It can also be used to prevent and treat hepatitis, diabetes, hyperlipidemia, and arteriosclerosis.

AIM OF THE STUDY

The present study was designed to compare the biological activities such as melanin synthesis inhibitory, anti-allergy, anti-lipase, anti-bacterial, anti-oxidant, and neuroprotective activities of different parts of the plant that may justify the use of this plant in folk medicine.

MATERIAL AND METHODS

The roots, stems, leaves and, seeds of Angelica shikokiana were separately extracted with water and ethanol. Each extract was examined for melanin synthesis inhibitory and anti-allergy activity on B16-melanoma and RBL-2H3 cells using IgE and A23187 as a stimulant for β-hexosaminidase release, respectively. We also evaluated the inhibition of two enzymes, lipase and acetylcholine esterase, and of the bacterial growth of two species, Escherichia coli and Staphylococcus aureaus. The anti-oxidant activity was determined using oxygen radical anti-oxidant capacity, ORAC assay and its relation to the phenolic content was estimated using the Folin-Ciocalteu method. Besides, the protective effect of the extracts against H2O2-induced oxidative stress in mouse neuroblastoma, Neuro-2A cells was investigated.

RESULTS

The most active extract exhibiting melanin synthesis inhibition (63%) and at the same time with low cytotoxicity (15%) was the ethanol extract of roots at 20 µg/ml, followed by the ethanol extract of stems (57% inhibition, 5% cytotoxicity). On the other hand, the highest inhibitions of β-hexosaminidase release were recorded for the ethanol extract of leaves with IC50 value of 6.89 µg/ml followed by the water extract of the seeds and leaves with IC50 value of 78.32 and 88.44 µg/ml, respectively. For anti-lipase assay, ethanol extracts of the stems and roots showed the strongest inhibition with IC50 values of 204.06 and 216.24 µg/ml, respectively. None of the examined extracts showed any activity against Escherichia coli. while the ethanol extract of the roots and stems showed moderate inhibition for Staphylococcus aureus with minimum inhibitory concentration of 400 µg/ml. Ethanol extract of the roots showed only 30% inhibition of acetylcholine esterase enzyme. The results of anti-oxidant, phenolic content and protective effect against H2O2-induced cytotoxicity assays showed highly correlated data. Ethanol extract of the stems (ORAC value of 1.08 µmol Trolox/mg and phenolic content 44.25 μg GAE/mg) increased the cell viability of H2O2-treated Neuro-2A cells by 28%.

Authors+Show Affiliations

Division of Systematic Forest and Forest Products Sciences, Department of agro-environmental sciences, Faculty of Agriculture, Graduate School of Kyushu University, 812-8581, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

23769982

Citation

Mira, Amira, et al. "Comparative Biological Study of Roots, Stems, Leaves, and Seeds of Angelica Shikokiana Makino." Journal of Ethnopharmacology, vol. 148, no. 3, 2013, pp. 980-7.
Mira A, Tanaka A, Tateyama Y, et al. Comparative biological study of roots, stems, leaves, and seeds of Angelica shikokiana Makino. J Ethnopharmacol. 2013;148(3):980-7.
Mira, A., Tanaka, A., Tateyama, Y., Kondo, R., & Shimizu, K. (2013). Comparative biological study of roots, stems, leaves, and seeds of Angelica shikokiana Makino. Journal of Ethnopharmacology, 148(3), 980-7. https://doi.org/10.1016/j.jep.2013.06.008
Mira A, et al. Comparative Biological Study of Roots, Stems, Leaves, and Seeds of Angelica Shikokiana Makino. J Ethnopharmacol. 2013 Jul 30;148(3):980-7. PubMed PMID: 23769982.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative biological study of roots, stems, leaves, and seeds of Angelica shikokiana Makino. AU - Mira,Amira, AU - Tanaka,Akinobu, AU - Tateyama,Yumie, AU - Kondo,Ryuichiro, AU - Shimizu,Kuniyoshi, Y1 - 2013/06/12/ PY - 2013/03/06/received PY - 2013/06/03/revised PY - 2013/06/05/accepted PY - 2013/6/18/entrez PY - 2013/6/19/pubmed PY - 2014/2/1/medline KW - 2,4-dinitrophenylated bovine serum albumin KW - 5,5-dithiobis[2-nitrobenzoic acid] KW - 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid KW - AAPH KW - AChE KW - AChEI KW - Angelica shikokiana KW - Anti-allergy KW - Anti-lipase KW - Anti-oxidant KW - DNP-BSA KW - DTNB KW - EMEM KW - Eagle's minimal essential medium KW - FBS KW - LE KW - LW KW - MTT KW - Melanin synthesis inhibitory KW - Neuro-2A KW - Neuroprotective KW - PBS KW - RBL KW - RE KW - RW KW - SE KW - STW KW - SW, water extract of seeds. STE KW - Trolox KW - [2,2′-azobis(2-amidino-propan) dihydrochloride] KW - acetylcholine esterase KW - acetylcholine esterase inhibitory KW - anti DNB KW - ethanol extract of leaves KW - ethanol extract of seeds KW - ethanol extract of stems KW - ethanol extract of the roots KW - fetal bovine serum KW - monoclonal anti-dinitrophenyl antibody KW - mouse neuroblastoma KW - phosphate-buffered saline KW - rat basophilic leukemia KW - thiazolyl blue tetrazolium bromide KW - water extract extract of roots KW - water extract extracts of leaves KW - water extract of stems SP - 980 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 148 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Angelica shikokiana has been used as a health food for its anticancer, anti-inflammatory, antibacterial, antiallergic, and blood vessel dilating effects in Japan. It can also be used to prevent and treat hepatitis, diabetes, hyperlipidemia, and arteriosclerosis. AIM OF THE STUDY: The present study was designed to compare the biological activities such as melanin synthesis inhibitory, anti-allergy, anti-lipase, anti-bacterial, anti-oxidant, and neuroprotective activities of different parts of the plant that may justify the use of this plant in folk medicine. MATERIAL AND METHODS: The roots, stems, leaves and, seeds of Angelica shikokiana were separately extracted with water and ethanol. Each extract was examined for melanin synthesis inhibitory and anti-allergy activity on B16-melanoma and RBL-2H3 cells using IgE and A23187 as a stimulant for β-hexosaminidase release, respectively. We also evaluated the inhibition of two enzymes, lipase and acetylcholine esterase, and of the bacterial growth of two species, Escherichia coli and Staphylococcus aureaus. The anti-oxidant activity was determined using oxygen radical anti-oxidant capacity, ORAC assay and its relation to the phenolic content was estimated using the Folin-Ciocalteu method. Besides, the protective effect of the extracts against H2O2-induced oxidative stress in mouse neuroblastoma, Neuro-2A cells was investigated. RESULTS: The most active extract exhibiting melanin synthesis inhibition (63%) and at the same time with low cytotoxicity (15%) was the ethanol extract of roots at 20 µg/ml, followed by the ethanol extract of stems (57% inhibition, 5% cytotoxicity). On the other hand, the highest inhibitions of β-hexosaminidase release were recorded for the ethanol extract of leaves with IC50 value of 6.89 µg/ml followed by the water extract of the seeds and leaves with IC50 value of 78.32 and 88.44 µg/ml, respectively. For anti-lipase assay, ethanol extracts of the stems and roots showed the strongest inhibition with IC50 values of 204.06 and 216.24 µg/ml, respectively. None of the examined extracts showed any activity against Escherichia coli. while the ethanol extract of the roots and stems showed moderate inhibition for Staphylococcus aureus with minimum inhibitory concentration of 400 µg/ml. Ethanol extract of the roots showed only 30% inhibition of acetylcholine esterase enzyme. The results of anti-oxidant, phenolic content and protective effect against H2O2-induced cytotoxicity assays showed highly correlated data. Ethanol extract of the stems (ORAC value of 1.08 µmol Trolox/mg and phenolic content 44.25 μg GAE/mg) increased the cell viability of H2O2-treated Neuro-2A cells by 28%. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23769982/Comparative_biological_study_of_roots_stems_leaves_and_seeds_of_Angelica_shikokiana_Makino_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00411-X DB - PRIME DP - Unbound Medicine ER -