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Identification of European-type hepatitis E virus subtype 3e isolates in Japanese wild boars: molecular tracing of HEV from swine to wild boars.
Infect Genet Evol. 2013 Aug; 18:287-98.IG

Abstract

Nucleotide sequences of hepatitis E virus (HEV) isolates infecting wild boars in Mie prefecture, which is located in the central region of Japan and is far from the most prevalent regions of HEV infection in Japan, were determined and characterised. Among 144 serum samples of wild boars captured in Mie prefecture, 7 were positive for HEV-RNA. The nucleotide sequence of nearly the entire genome was determined for 4 of the 7 positive samples. Phylogenetic tree analyses indicated that 6 samples were subtype 3e and 1 was subtype 3a among the 7 isolates. We identified the indigenization of subtype 3e isolates in Japanese wild boars. Furthermore, 5 subtype 3e isolates were closely related and were located in the peripheral branch of subtype 3e isolates from European countries in the phylogenetic tree. The structure indicated that the ancestor of the 5 subtype 3e isolates originated in Europe. The phylogenetic structure and coalescent analyses suggested that the subtype 3e isolates entered Japan from Europe by importation of large-race pigs around 1966. The results also indicated that several lineages of subtype 3e expanded to a wide area of Japan around 1992 and 1 of the lineages was indigenized in wild boars in Mie prefecture between 1992 and 2009. The appearance of a wild boar cluster in the peripheral branch in the phylogenetic lineage may indicate the direction of gene flow of HEV subtype 3e from swine to wild boars. Clarification of the transmission direction or route should be helpful to prevent a future endemic or epidemic of HEV infection.

Authors+Show Affiliations

Department of Internal Medicine, Fujita Health University Nanakuri Sanatorium, Mie 514-1295, Japan. tanakano@sf.starcat.ne.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23770142

Citation

Nakano, Tatsunori, et al. "Identification of European-type Hepatitis E Virus Subtype 3e Isolates in Japanese Wild Boars: Molecular Tracing of HEV From Swine to Wild Boars." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 18, 2013, pp. 287-98.
Nakano T, Takahashi K, Arai M, et al. Identification of European-type hepatitis E virus subtype 3e isolates in Japanese wild boars: molecular tracing of HEV from swine to wild boars. Infect Genet Evol. 2013;18:287-98.
Nakano, T., Takahashi, K., Arai, M., Okano, H., Kato, H., Ayada, M., Okamoto, H., & Mishiro, S. (2013). Identification of European-type hepatitis E virus subtype 3e isolates in Japanese wild boars: molecular tracing of HEV from swine to wild boars. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 18, 287-98. https://doi.org/10.1016/j.meegid.2013.06.004
Nakano T, et al. Identification of European-type Hepatitis E Virus Subtype 3e Isolates in Japanese Wild Boars: Molecular Tracing of HEV From Swine to Wild Boars. Infect Genet Evol. 2013;18:287-98. PubMed PMID: 23770142.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of European-type hepatitis E virus subtype 3e isolates in Japanese wild boars: molecular tracing of HEV from swine to wild boars. AU - Nakano,Tatsunori, AU - Takahashi,Kazuaki, AU - Arai,Masahiro, AU - Okano,Hiroshi, AU - Kato,Hideaki, AU - Ayada,Minoru, AU - Okamoto,Hiroaki, AU - Mishiro,Shunji, Y1 - 2013/06/12/ PY - 2013/04/14/received PY - 2013/06/01/revised PY - 2013/06/03/accepted PY - 2013/6/18/entrez PY - 2013/6/19/pubmed PY - 2014/4/25/medline KW - BF KW - Bayes factor KW - Genotype KW - HEV KW - Hepatitis E virus KW - Japan KW - MCMC KW - Markov chain Monte Carlo KW - Phylogenetic analysis KW - Subtype 3e KW - TMRCA KW - Wild boar KW - hepatitis E virus KW - time of the most recent common ancestor SP - 287 EP - 98 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect Genet Evol VL - 18 N2 - Nucleotide sequences of hepatitis E virus (HEV) isolates infecting wild boars in Mie prefecture, which is located in the central region of Japan and is far from the most prevalent regions of HEV infection in Japan, were determined and characterised. Among 144 serum samples of wild boars captured in Mie prefecture, 7 were positive for HEV-RNA. The nucleotide sequence of nearly the entire genome was determined for 4 of the 7 positive samples. Phylogenetic tree analyses indicated that 6 samples were subtype 3e and 1 was subtype 3a among the 7 isolates. We identified the indigenization of subtype 3e isolates in Japanese wild boars. Furthermore, 5 subtype 3e isolates were closely related and were located in the peripheral branch of subtype 3e isolates from European countries in the phylogenetic tree. The structure indicated that the ancestor of the 5 subtype 3e isolates originated in Europe. The phylogenetic structure and coalescent analyses suggested that the subtype 3e isolates entered Japan from Europe by importation of large-race pigs around 1966. The results also indicated that several lineages of subtype 3e expanded to a wide area of Japan around 1992 and 1 of the lineages was indigenized in wild boars in Mie prefecture between 1992 and 2009. The appearance of a wild boar cluster in the peripheral branch in the phylogenetic lineage may indicate the direction of gene flow of HEV subtype 3e from swine to wild boars. Clarification of the transmission direction or route should be helpful to prevent a future endemic or epidemic of HEV infection. SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/23770142/Identification_of_European_type_hepatitis_E_virus_subtype_3e_isolates_in_Japanese_wild_boars:_molecular_tracing_of_HEV_from_swine_to_wild_boars_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-1348(13)00229-3 DB - PRIME DP - Unbound Medicine ER -