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Antioxidant properties and neuroprotective effects of isocampneoside II on hydrogen peroxide-induced oxidative injury in PC12 cells.
Food Chem Toxicol. 2013 Sep; 59:145-52.FC

Abstract

Oxidative stress has been considered as a major cause of cell damage in various neurodegenerative disorders. One of the reasonable strategies for delaying the disease's progression is to prevent reactive oxygen species (ROS) mediated cellular injury by dietary or pharmaceutical augmentation of free radical scavengers. Isocampneoside II (ICD) is an active phenylethanoid glycoside isolated from the medicinal hardwood genus Paulownia. This study was designed to explore free radical scavenging potential of ICD in different in vitro systems and its protective role in hydrogen peroxide (H₂O₂)-induced oxidative stress and apoptotic death in cultured rat pheochromocytoma (PC12) cells. The results showed ICD eliminated approximately 80.75% superoxide radical at the concentration of 0.1mg/ml and inhibited metal chelating by 22.07% at 8 mg/ml. Additionally, ICD showed a strong ability on reducing power and provided protection against oxidative protein damage induced by hydroxyl radicals. Pretreatment of PC12 cells with ICD prior to H₂O₂ exposure elevated cell viability, enhanced activity of superoxide dismutase and catalase, and decreased levels of malondialdehyde and intracellular ROS. Furthermore, ICD inhibited cell apoptosis and Bax/Bcl-2 ratio induced by H₂O₂. These findings suggested ICD may be considered as a potential antioxidant agent and should encourage for further research in neurodegenerative diseases.

Authors+Show Affiliations

Tianjin Key Laboratory of Pulp & Paper, College of Materials Science & Chemical Engineering, Tianjin University of Science & Technology, Tianjin 300457, PR China. sichli@tust.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23770344

Citation

Si, Chuan-Ling, et al. "Antioxidant Properties and Neuroprotective Effects of Isocampneoside II On Hydrogen Peroxide-induced Oxidative Injury in PC12 Cells." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 59, 2013, pp. 145-52.
Si CL, Shen T, Jiang YY, et al. Antioxidant properties and neuroprotective effects of isocampneoside II on hydrogen peroxide-induced oxidative injury in PC12 cells. Food Chem Toxicol. 2013;59:145-52.
Si, C. L., Shen, T., Jiang, Y. Y., Wu, L., Yu, G. J., Ren, X. D., Xu, G. H., & Hu, W. C. (2013). Antioxidant properties and neuroprotective effects of isocampneoside II on hydrogen peroxide-induced oxidative injury in PC12 cells. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 59, 145-52. https://doi.org/10.1016/j.fct.2013.05.051
Si CL, et al. Antioxidant Properties and Neuroprotective Effects of Isocampneoside II On Hydrogen Peroxide-induced Oxidative Injury in PC12 Cells. Food Chem Toxicol. 2013;59:145-52. PubMed PMID: 23770344.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant properties and neuroprotective effects of isocampneoside II on hydrogen peroxide-induced oxidative injury in PC12 cells. AU - Si,Chuan-Ling, AU - Shen,Ting, AU - Jiang,Yun-Yao, AU - Wu,Lei, AU - Yu,Guo-Jing, AU - Ren,Xiao-Dan, AU - Xu,Guang-Hui, AU - Hu,Wei-Cheng, Y1 - 2013/06/11/ PY - 2013/05/02/received PY - 2013/05/29/revised PY - 2013/05/30/accepted PY - 2013/6/18/entrez PY - 2013/6/19/pubmed PY - 2014/3/29/medline KW - Antioxidant KW - Isocampneoside II KW - Malondialdehyde KW - Neurodegeneration KW - Reactive oxygen species SP - 145 EP - 52 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 59 N2 - Oxidative stress has been considered as a major cause of cell damage in various neurodegenerative disorders. One of the reasonable strategies for delaying the disease's progression is to prevent reactive oxygen species (ROS) mediated cellular injury by dietary or pharmaceutical augmentation of free radical scavengers. Isocampneoside II (ICD) is an active phenylethanoid glycoside isolated from the medicinal hardwood genus Paulownia. This study was designed to explore free radical scavenging potential of ICD in different in vitro systems and its protective role in hydrogen peroxide (H₂O₂)-induced oxidative stress and apoptotic death in cultured rat pheochromocytoma (PC12) cells. The results showed ICD eliminated approximately 80.75% superoxide radical at the concentration of 0.1mg/ml and inhibited metal chelating by 22.07% at 8 mg/ml. Additionally, ICD showed a strong ability on reducing power and provided protection against oxidative protein damage induced by hydroxyl radicals. Pretreatment of PC12 cells with ICD prior to H₂O₂ exposure elevated cell viability, enhanced activity of superoxide dismutase and catalase, and decreased levels of malondialdehyde and intracellular ROS. Furthermore, ICD inhibited cell apoptosis and Bax/Bcl-2 ratio induced by H₂O₂. These findings suggested ICD may be considered as a potential antioxidant agent and should encourage for further research in neurodegenerative diseases. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/23770344/Antioxidant_properties_and_neuroprotective_effects_of_isocampneoside_II_on_hydrogen_peroxide_induced_oxidative_injury_in_PC12_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(13)00357-8 DB - PRIME DP - Unbound Medicine ER -