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Total glucosides of peony attenuates 2,4,6-trinitrobenzene sulfonic acid/ethanol-induced colitis in rats through adjustment of TH1/TH2 cytokines polarization.
Cell Biochem Biophys. 2014 Jan; 68(1):83-95.CB

Abstract

The present study is to investigate effects of total glucosides of peony (TGP) on 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol-induced colitis in rats and to explore potential clinical use of TGP for treatment of inflammatory bowel disease. Sixty Sprague-Dawley rats were randomly grouped into normal controls, model controls, sulfasalazine (SASP) controls (100 mg/kg/day), and low, medium, and high-dose TGP groups (25, 50, and 100 mg/kg/day, respectively). 24 h following colonic instillation of TNBS, TGP, and SASP were given by gastric gavage three times a day for 7 days. Disease activity index (DAI), colon macroscopic damage index (CMDI), histopathological score (HPS), and myeloperoxidase (MPO) activity were evaluated. Levels of serum TNF-α, IL-1β, and IL-10 were measured by ELISA, and expression of TNF-α, IL-1β, and IL-10 mRNA and protein in colonic tissues was detected by RT-PCR and western blot, respectively. Compared with rats in the model controls, TGP (50 or 100 mg/kg/day)-treated rats with TNBS/ethanol-induced colitis showed significant improvements of DAI, CMDI, HPS, and MPO activity. Moreover, administration of TGP (50 or 100 mg/kg/day) decreased the up-regulated levels of serum TNF-α and IL-1β, and expression of TNF-α and IL-1β mRNA and protein in colonic tissues, and increased the serum IL-10 and colonic IL-10 mRNA and protein level. And there was no significant difference compared with administration of SASP (P > 0.05). TGP attenuates TNBS/ethanol-induced colitis in rats and its efficacy is similar to SASP, the potential mechanism might be related to the adjustment of Th1/Th2 cytokines polarization by decreasing pro-inflammatory cytokine TNF-α and IL-1β, and increasing anti-inflammatory cytokine IL-10.

Authors+Show Affiliations

Department of Gastroenterology, Zhongnan Hospital of Wuhan University School of Medicine, Donghu Road 169, Wuhan, 430071, Hubei, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23771723

Citation

Zhang, Yabing, et al. "Total Glucosides of Peony Attenuates 2,4,6-trinitrobenzene Sulfonic Acid/ethanol-induced Colitis in Rats Through Adjustment of TH1/TH2 Cytokines Polarization." Cell Biochemistry and Biophysics, vol. 68, no. 1, 2014, pp. 83-95.
Zhang Y, Zhou R, Zhou F, et al. Total glucosides of peony attenuates 2,4,6-trinitrobenzene sulfonic acid/ethanol-induced colitis in rats through adjustment of TH1/TH2 cytokines polarization. Cell Biochem Biophys. 2014;68(1):83-95.
Zhang, Y., Zhou, R., Zhou, F., Cheng, H., & Xia, B. (2014). Total glucosides of peony attenuates 2,4,6-trinitrobenzene sulfonic acid/ethanol-induced colitis in rats through adjustment of TH1/TH2 cytokines polarization. Cell Biochemistry and Biophysics, 68(1), 83-95. https://doi.org/10.1007/s12013-013-9696-3
Zhang Y, et al. Total Glucosides of Peony Attenuates 2,4,6-trinitrobenzene Sulfonic Acid/ethanol-induced Colitis in Rats Through Adjustment of TH1/TH2 Cytokines Polarization. Cell Biochem Biophys. 2014;68(1):83-95. PubMed PMID: 23771723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Total glucosides of peony attenuates 2,4,6-trinitrobenzene sulfonic acid/ethanol-induced colitis in rats through adjustment of TH1/TH2 cytokines polarization. AU - Zhang,Yabing, AU - Zhou,Rui, AU - Zhou,Feng, AU - Cheng,Hong, AU - Xia,Bing, PY - 2013/6/18/entrez PY - 2013/6/19/pubmed PY - 2014/9/24/medline SP - 83 EP - 95 JF - Cell biochemistry and biophysics JO - Cell Biochem. Biophys. VL - 68 IS - 1 N2 - The present study is to investigate effects of total glucosides of peony (TGP) on 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol-induced colitis in rats and to explore potential clinical use of TGP for treatment of inflammatory bowel disease. Sixty Sprague-Dawley rats were randomly grouped into normal controls, model controls, sulfasalazine (SASP) controls (100 mg/kg/day), and low, medium, and high-dose TGP groups (25, 50, and 100 mg/kg/day, respectively). 24 h following colonic instillation of TNBS, TGP, and SASP were given by gastric gavage three times a day for 7 days. Disease activity index (DAI), colon macroscopic damage index (CMDI), histopathological score (HPS), and myeloperoxidase (MPO) activity were evaluated. Levels of serum TNF-α, IL-1β, and IL-10 were measured by ELISA, and expression of TNF-α, IL-1β, and IL-10 mRNA and protein in colonic tissues was detected by RT-PCR and western blot, respectively. Compared with rats in the model controls, TGP (50 or 100 mg/kg/day)-treated rats with TNBS/ethanol-induced colitis showed significant improvements of DAI, CMDI, HPS, and MPO activity. Moreover, administration of TGP (50 or 100 mg/kg/day) decreased the up-regulated levels of serum TNF-α and IL-1β, and expression of TNF-α and IL-1β mRNA and protein in colonic tissues, and increased the serum IL-10 and colonic IL-10 mRNA and protein level. And there was no significant difference compared with administration of SASP (P > 0.05). TGP attenuates TNBS/ethanol-induced colitis in rats and its efficacy is similar to SASP, the potential mechanism might be related to the adjustment of Th1/Th2 cytokines polarization by decreasing pro-inflammatory cytokine TNF-α and IL-1β, and increasing anti-inflammatory cytokine IL-10. SN - 1559-0283 UR - https://www.unboundmedicine.com/medline/citation/23771723/Total_glucosides_of_peony_attenuates_246_trinitrobenzene_sulfonic_acid/ethanol_induced_colitis_in_rats_through_adjustment_of_TH1/TH2_cytokines_polarization_ L2 - https://dx.doi.org/10.1007/s12013-013-9696-3 DB - PRIME DP - Unbound Medicine ER -