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Simultaneous quantification of six main active constituents in Chinese Angelica by high-performance liquid chromatography with photodiode array detector.
Pharmacogn Mag. 2013 Apr; 9(34):114-9.PM

Abstract

BACKGROUND

Angelica sinensis is a famous traditional Chinese medicinalherb, which is predominantly used in the treatment of gynecological conditions. It is the first report for the simultaneous determination of six major active components in Chinese Angelica, which is important for quality control.

OBJECTIVE

A validated HPLC-PAD method was first developed to evaluate the quality of crude and processed Radix Angelica through simultaneous determination of six bioactive compounds, namely ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, Z/E-ligustilide and Z/E-butylidenephthalide.

MATERIALS AND METHODS

Samples were separated on a Xtimate™C18 column (250 × 4.6 mm, 5 μm) and detected by PAD. Mobile phase was composed of (A) aqueous phosphoric acid (0.02%, v/v) and (B) acetonitrile (MeCN) (including 10% tetrahydrofuran, v/v) using a gradient elution. Analytes were performed at 30°C with a flow rate of 1.0 mL/min.

RESULTS

All calibration curves showed good linear regression (r(2) ≥ 0.9963) within the tested ranges, and the recovery of the method was in the range of 91.927-105.859%.

CONCLUSION

The results demonstrate that the developed method is accurate and reproducible and could be readily utilized as a suitable quality control method for the quantification of Radix Angelica.

Authors+Show Affiliations

Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23772106

Citation

Ying, Li, et al. "Simultaneous Quantification of Six Main Active Constituents in Chinese Angelica By High-performance Liquid Chromatography With Photodiode Array Detector." Pharmacognosy Magazine, vol. 9, no. 34, 2013, pp. 114-9.
Ying L, Si-Wang W, Hong-Hai T, et al. Simultaneous quantification of six main active constituents in Chinese Angelica by high-performance liquid chromatography with photodiode array detector. Pharmacogn Mag. 2013;9(34):114-9.
Ying, L., Si-Wang, W., Hong-Hai, T., & Wei, C. (2013). Simultaneous quantification of six main active constituents in Chinese Angelica by high-performance liquid chromatography with photodiode array detector. Pharmacognosy Magazine, 9(34), 114-9. https://doi.org/10.4103/0973-1296.111255
Ying L, et al. Simultaneous Quantification of Six Main Active Constituents in Chinese Angelica By High-performance Liquid Chromatography With Photodiode Array Detector. Pharmacogn Mag. 2013;9(34):114-9. PubMed PMID: 23772106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of six main active constituents in Chinese Angelica by high-performance liquid chromatography with photodiode array detector. AU - Ying,Li, AU - Si-Wang,Wang, AU - Hong-Hai,Tu, AU - Wei,Cao, PY - 2012/03/19/received PY - 2012/05/26/revised PY - 2013/04/30/accepted PY - 2013/6/18/entrez PY - 2013/6/19/pubmed PY - 2013/6/19/medline KW - Chinese Angelica KW - high performance liquid chromatography KW - photodiode-array detector KW - quality control KW - simultaneous determination SP - 114 EP - 9 JF - Pharmacognosy magazine JO - Pharmacogn Mag VL - 9 IS - 34 N2 - BACKGROUND: Angelica sinensis is a famous traditional Chinese medicinalherb, which is predominantly used in the treatment of gynecological conditions. It is the first report for the simultaneous determination of six major active components in Chinese Angelica, which is important for quality control. OBJECTIVE: A validated HPLC-PAD method was first developed to evaluate the quality of crude and processed Radix Angelica through simultaneous determination of six bioactive compounds, namely ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, Z/E-ligustilide and Z/E-butylidenephthalide. MATERIALS AND METHODS: Samples were separated on a Xtimate™C18 column (250 × 4.6 mm, 5 μm) and detected by PAD. Mobile phase was composed of (A) aqueous phosphoric acid (0.02%, v/v) and (B) acetonitrile (MeCN) (including 10% tetrahydrofuran, v/v) using a gradient elution. Analytes were performed at 30°C with a flow rate of 1.0 mL/min. RESULTS: All calibration curves showed good linear regression (r(2) ≥ 0.9963) within the tested ranges, and the recovery of the method was in the range of 91.927-105.859%. CONCLUSION: The results demonstrate that the developed method is accurate and reproducible and could be readily utilized as a suitable quality control method for the quantification of Radix Angelica. SN - 0973-1296 UR - https://www.unboundmedicine.com/medline/citation/23772106/Simultaneous_quantification_of_six_main_active_constituents_in_Chinese_Angelica_by_high_performance_liquid_chromatography_with_photodiode_array_detector_ L2 - http://www.phcog.com/article.asp?issn=0973-1296;year=2013;volume=9;issue=34;spage=114;epage=119;aulast=Ying DB - PRIME DP - Unbound Medicine ER -
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