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Q fever--selected issues.
Ann Agric Environ Med. 2013; 20(2):222-32.AA

Abstract

Q fever is an infectious disease of humans and animals caused by Gram-negative coccobacillus Coxiella burnetii, belonging to the Legionellales order, Coxiellaceae family. The presented study compares selected features of the bacteria genome, including chromosome and plasmids QpH1, QpRS, QpDG and QpDV. The pathomechanism of infection--starting from internalization of the bacteria to its release from infected cell are thoroughly described. The drugs of choice for the treatment of acute Q fever are tetracyclines, macrolides and quinolones. Some other antimicrobials are also active against C. burnetii, namely, telitromycines and tigecyclines (glicylcycline). Q-VAX vaccine induces strong and long-term immunity in humans. Coxevac vaccine for goat and sheep can reduce the number of infections and abortions, as well as decrease the environmental transmission of the pathogen. Using the microarrays technique, about 50 proteins has been identified which could be used in the future for the production of vaccine against Q fever. The routine method of C. burnetii culture is proliferation within cell lines; however, an artificial culture medium has recently been developed. The growth of bacteria in a reduced oxygen (2.5%) atmosphere was obtained after just 6 days. In serology, using the IF method as positive titers, the IgM antibody level >1:64 and IgG antibody level >1:256 (against II phase antigens) has been considered. In molecular diagnostics of C. burnetii infection, the most frequently used method is PCR and its modifications; namely, nested PCR and real time PCR which detect target sequences, such as htpAB and IS1111, chromosome genes (com1), genes specific for different types of plasmids and transposase genes. Although Q fever was diagnosed in Poland in 1956, the data about the occurrence of the disease are incomplete. Comprehensive studies on the current status of Q fever in Poland, with special focus on pathogen reservoirs and vectors, the sources of infection and molecular characteristics of bacteria should be conducted.

Authors+Show Affiliations

Biological Threats Identification and Countermeasure Center of the Military Institute of Hygiene and Epidemiology, Pulawy, Poland. abdr@wp.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

23772566

Citation

Bielawska-Drózd, Agata, et al. "Q Fever--selected Issues." Annals of Agricultural and Environmental Medicine : AAEM, vol. 20, no. 2, 2013, pp. 222-32.
Bielawska-Drózd A, Cieślik P, Mirski T, et al. Q fever--selected issues. Ann Agric Environ Med. 2013;20(2):222-32.
Bielawska-Drózd, A., Cieślik, P., Mirski, T., Bartoszcze, M., Knap, J. P., Gaweł, J., & Żakowska, D. (2013). Q fever--selected issues. Annals of Agricultural and Environmental Medicine : AAEM, 20(2), 222-32.
Bielawska-Drózd A, et al. Q Fever--selected Issues. Ann Agric Environ Med. 2013;20(2):222-32. PubMed PMID: 23772566.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Q fever--selected issues. AU - Bielawska-Drózd,Agata, AU - Cieślik,Piotr, AU - Mirski,Tomasz, AU - Bartoszcze,Michał, AU - Knap,Józef Piotr, AU - Gaweł,Jerzy, AU - Żakowska,Dorota, PY - 2013/6/19/entrez PY - 2013/6/19/pubmed PY - 2014/1/23/medline SP - 222 EP - 32 JF - Annals of agricultural and environmental medicine : AAEM JO - Ann Agric Environ Med VL - 20 IS - 2 N2 - Q fever is an infectious disease of humans and animals caused by Gram-negative coccobacillus Coxiella burnetii, belonging to the Legionellales order, Coxiellaceae family. The presented study compares selected features of the bacteria genome, including chromosome and plasmids QpH1, QpRS, QpDG and QpDV. The pathomechanism of infection--starting from internalization of the bacteria to its release from infected cell are thoroughly described. The drugs of choice for the treatment of acute Q fever are tetracyclines, macrolides and quinolones. Some other antimicrobials are also active against C. burnetii, namely, telitromycines and tigecyclines (glicylcycline). Q-VAX vaccine induces strong and long-term immunity in humans. Coxevac vaccine for goat and sheep can reduce the number of infections and abortions, as well as decrease the environmental transmission of the pathogen. Using the microarrays technique, about 50 proteins has been identified which could be used in the future for the production of vaccine against Q fever. The routine method of C. burnetii culture is proliferation within cell lines; however, an artificial culture medium has recently been developed. The growth of bacteria in a reduced oxygen (2.5%) atmosphere was obtained after just 6 days. In serology, using the IF method as positive titers, the IgM antibody level >1:64 and IgG antibody level >1:256 (against II phase antigens) has been considered. In molecular diagnostics of C. burnetii infection, the most frequently used method is PCR and its modifications; namely, nested PCR and real time PCR which detect target sequences, such as htpAB and IS1111, chromosome genes (com1), genes specific for different types of plasmids and transposase genes. Although Q fever was diagnosed in Poland in 1956, the data about the occurrence of the disease are incomplete. Comprehensive studies on the current status of Q fever in Poland, with special focus on pathogen reservoirs and vectors, the sources of infection and molecular characteristics of bacteria should be conducted. SN - 1898-2263 UR - https://www.unboundmedicine.com/medline/citation/23772566/Q_fever__selected_issues_ L2 - http://www.aaem.pl/fulltext.php?ICID=1052321 DB - PRIME DP - Unbound Medicine ER -