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pDB2011, a 7.6 kb multidrug resistance plasmid from Listeria innocua replicating in Gram-positive and Gram-negative hosts.
Plasmid 2013; 70(2):284-7P

Abstract

pDB2011, a multidrug resistance plasmid isolated from the foodborne Listeria innocua strain TTS-2011 was sequenced and characterized. Sequence analysis revealed that pDB2011 had a length of 7641 bp and contained seven coding DNA sequences of which two were annotated as replication proteins, one as a recombination/mobilization protein and one as a transposase. Furthermore, pDB2011 harbored the trimethoprim, spectinomycin and macrolide-lincosamide-streptogramin B resistance genes dfrD, spc and erm(A), respectively. However, pDB2011 was only associated with trimethoprim and spectinomycin resistance phenotypes and not with phenotypic resistance to erythromycin. A region of the plasmid encoding the resistance genes spc and erm(A) plus the transposase was highly similar to Staphylococcus aureus transposon Tn554. The dfrD gene was 100% identical to dfrD found in a number of Listeria monocytogenes isolates. Additionally, assessment of the potential host range of pDB2011 revealed that the plasmid was able to replicate in Lactococcus lactis subsp. cremoris MG1363 as well as in Escherichia coli MC1061 and DH5α. This study reports the first multidrug resistance plasmid in L. innocua. A large potential for dissemination of pDB2011 is indicated by its host range of both Gram-positive and Gram-negative bacteria.

Authors+Show Affiliations

Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23774482

Citation

Bertsch, David, et al. "PDB2011, a 7.6 Kb Multidrug Resistance Plasmid From Listeria Innocua Replicating in Gram-positive and Gram-negative Hosts." Plasmid, vol. 70, no. 2, 2013, pp. 284-7.
Bertsch D, Anderegg J, Lacroix C, et al. PDB2011, a 7.6 kb multidrug resistance plasmid from Listeria innocua replicating in Gram-positive and Gram-negative hosts. Plasmid. 2013;70(2):284-7.
Bertsch, D., Anderegg, J., Lacroix, C., Meile, L., & Stevens, M. J. (2013). PDB2011, a 7.6 kb multidrug resistance plasmid from Listeria innocua replicating in Gram-positive and Gram-negative hosts. Plasmid, 70(2), pp. 284-7. doi:10.1016/j.plasmid.2013.06.001.
Bertsch D, et al. PDB2011, a 7.6 Kb Multidrug Resistance Plasmid From Listeria Innocua Replicating in Gram-positive and Gram-negative Hosts. Plasmid. 2013;70(2):284-7. PubMed PMID: 23774482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - pDB2011, a 7.6 kb multidrug resistance plasmid from Listeria innocua replicating in Gram-positive and Gram-negative hosts. AU - Bertsch,David, AU - Anderegg,Janine, AU - Lacroix,Christophe, AU - Meile,Leo, AU - Stevens,Marc J A, Y1 - 2013/06/14/ PY - 2013/03/26/received PY - 2013/06/04/revised PY - 2013/06/06/accepted PY - 2013/6/19/entrez PY - 2013/6/19/pubmed PY - 2014/2/19/medline KW - Broad-host-range plasmid KW - Macrolide-lincosamide-streptogramin B resistance KW - Spectinomycin resistance KW - Trimethoprim resistance KW - dfrD KW - erm(A) SP - 284 EP - 7 JF - Plasmid JO - Plasmid VL - 70 IS - 2 N2 - pDB2011, a multidrug resistance plasmid isolated from the foodborne Listeria innocua strain TTS-2011 was sequenced and characterized. Sequence analysis revealed that pDB2011 had a length of 7641 bp and contained seven coding DNA sequences of which two were annotated as replication proteins, one as a recombination/mobilization protein and one as a transposase. Furthermore, pDB2011 harbored the trimethoprim, spectinomycin and macrolide-lincosamide-streptogramin B resistance genes dfrD, spc and erm(A), respectively. However, pDB2011 was only associated with trimethoprim and spectinomycin resistance phenotypes and not with phenotypic resistance to erythromycin. A region of the plasmid encoding the resistance genes spc and erm(A) plus the transposase was highly similar to Staphylococcus aureus transposon Tn554. The dfrD gene was 100% identical to dfrD found in a number of Listeria monocytogenes isolates. Additionally, assessment of the potential host range of pDB2011 revealed that the plasmid was able to replicate in Lactococcus lactis subsp. cremoris MG1363 as well as in Escherichia coli MC1061 and DH5α. This study reports the first multidrug resistance plasmid in L. innocua. A large potential for dissemination of pDB2011 is indicated by its host range of both Gram-positive and Gram-negative bacteria. SN - 1095-9890 UR - https://www.unboundmedicine.com/medline/citation/23774482/pDB2011_a_7_6_kb_multidrug_resistance_plasmid_from_Listeria_innocua_replicating_in_Gram_positive_and_Gram_negative_hosts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-619X(13)00075-9 DB - PRIME DP - Unbound Medicine ER -