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Increased resistance against citrus canker mediated by a citrus mitogen-activated protein kinase.
Mol Plant Microbe Interact. 2013 Oct; 26(10):1190-9.MP

Abstract

Mitogen-activated protein kinases (MAPK) play crucial roles in plant immunity. We previously identified a citrus MAPK (CsMAPK1) as a differentially expressed protein in response to infection by Xanthomonas aurantifolii, a bacterium that causes citrus canker in Mexican lime but a hypersensitive reaction in sweet oranges. Here, we confirm that, in sweet orange, CsMAPK1 is rapidly and preferentially induced by X. aurantifolii relative to Xanthomonas citri. To investigate the role of CsMAPK1 in citrus canker resistance, we expressed CsMAPK1 in citrus plants under the control of the PR5 gene promoter, which is induced by Xanthomonas infection and wounding. Increased expression of CsMAPK1 correlated with a reduction in canker symptoms and a decrease in bacterial growth. Canker lesions in plants with higher CsMAPK1 levels were smaller and showed fewer signs of epidermal rupture. Transgenic plants also revealed higher transcript levels of defense-related genes and a significant accumulation of hydrogen peroxide in response to wounding or X. citri infection. Accordingly, nontransgenic sweet orange leaves accumulate both CsMAPK1 and hydrogen peroxide in response to X. aurantifolii but not X. citri infection. These data, thus, indicate that CsMAPK1 functions in the citrus canker defense response by inducing defense gene expression and reactive oxygen species accumulation during infection.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23777433

Citation

de Oliveira, Maria Luiza Peixoto, et al. "Increased Resistance Against Citrus Canker Mediated By a Citrus Mitogen-activated Protein Kinase." Molecular Plant-microbe Interactions : MPMI, vol. 26, no. 10, 2013, pp. 1190-9.
de Oliveira ML, de Lima Silva CC, Abe VY, et al. Increased resistance against citrus canker mediated by a citrus mitogen-activated protein kinase. Mol Plant Microbe Interact. 2013;26(10):1190-9.
de Oliveira, M. L., de Lima Silva, C. C., Abe, V. Y., Costa, M. G., Cernadas, R. A., & Benedetti, C. E. (2013). Increased resistance against citrus canker mediated by a citrus mitogen-activated protein kinase. Molecular Plant-microbe Interactions : MPMI, 26(10), 1190-9. https://doi.org/10.1094/MPMI-04-13-0122-R
de Oliveira ML, et al. Increased Resistance Against Citrus Canker Mediated By a Citrus Mitogen-activated Protein Kinase. Mol Plant Microbe Interact. 2013;26(10):1190-9. PubMed PMID: 23777433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased resistance against citrus canker mediated by a citrus mitogen-activated protein kinase. AU - de Oliveira,Maria Luiza Peixoto, AU - de Lima Silva,Caio Cesar, AU - Abe,Valéria Yukari, AU - Costa,Marcio Gilberto Cardoso, AU - Cernadas,Raúl Andrés, AU - Benedetti,Celso Eduardo, PY - 2013/6/20/entrez PY - 2013/6/20/pubmed PY - 2014/2/7/medline SP - 1190 EP - 9 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 26 IS - 10 N2 - Mitogen-activated protein kinases (MAPK) play crucial roles in plant immunity. We previously identified a citrus MAPK (CsMAPK1) as a differentially expressed protein in response to infection by Xanthomonas aurantifolii, a bacterium that causes citrus canker in Mexican lime but a hypersensitive reaction in sweet oranges. Here, we confirm that, in sweet orange, CsMAPK1 is rapidly and preferentially induced by X. aurantifolii relative to Xanthomonas citri. To investigate the role of CsMAPK1 in citrus canker resistance, we expressed CsMAPK1 in citrus plants under the control of the PR5 gene promoter, which is induced by Xanthomonas infection and wounding. Increased expression of CsMAPK1 correlated with a reduction in canker symptoms and a decrease in bacterial growth. Canker lesions in plants with higher CsMAPK1 levels were smaller and showed fewer signs of epidermal rupture. Transgenic plants also revealed higher transcript levels of defense-related genes and a significant accumulation of hydrogen peroxide in response to wounding or X. citri infection. Accordingly, nontransgenic sweet orange leaves accumulate both CsMAPK1 and hydrogen peroxide in response to X. aurantifolii but not X. citri infection. These data, thus, indicate that CsMAPK1 functions in the citrus canker defense response by inducing defense gene expression and reactive oxygen species accumulation during infection. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/23777433/Increased_resistance_against_citrus_canker_mediated_by_a_citrus_mitogen_activated_protein_kinase_ L2 - https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-13-0122-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -