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Phylogeny-based classification of human rhinoviruses detected in hospitalized children with acute lower respiratory infection in Paraguay, 2010-2011.
J Med Virol. 2013 Sep; 85(9):1645-51.JM

Abstract

Human rhinovirus (HRV), a single-stranded, positive-sense RNA virus, is associated with mild upper respiratory tract infections in children. The aim of this study was to carry out a molecular characterization and phylogeny-based classification of the circulating genotypes of HRV in hospitalized children with clinical manifestations of acute lower respiratory infection in Paraguay. Nasopharyngeal aspirates were collected from 101 children under 5 years of age, hospitalized with symptoms of acute lower respiratory infection, between May 2010 and December 2011, at the largest public pediatric hospital in the Central Department of Paraguay. Detection was performed by a real-time polymerase chain reaction, followed by conventional amplification of the VP4/VP2 genomic region, sequencing, and phylogenetic analysis. Rhinovirus was detected in 33.7% of the samples. Amplification of 18 samples showed the presence of all three species (HRV-A, -B, and -C). Different genotypes were found for each species: 11 for HRV-A (-9, -12, -22, -30, -36, -43, -59, -61, -68, -88, and -89), one for HRV-B (-4), and four for HRV-C (-C2, -C3, -C6, and -C9). In South America, information about HRV diversity is scarce. This is the first report on HRV genotype diversity in South America.

Authors+Show Affiliations

Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Paraguay. emilioespinola@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23780670

Citation

Espínola, Emilio E., et al. "Phylogeny-based Classification of Human Rhinoviruses Detected in Hospitalized Children With Acute Lower Respiratory Infection in Paraguay, 2010-2011." Journal of Medical Virology, vol. 85, no. 9, 2013, pp. 1645-51.
Espínola EE, Russomando G, Aquino C, et al. Phylogeny-based classification of human rhinoviruses detected in hospitalized children with acute lower respiratory infection in Paraguay, 2010-2011. J Med Virol. 2013;85(9):1645-51.
Espínola, E. E., Russomando, G., Aquino, C., & Basualdo, W. (2013). Phylogeny-based classification of human rhinoviruses detected in hospitalized children with acute lower respiratory infection in Paraguay, 2010-2011. Journal of Medical Virology, 85(9), 1645-51. https://doi.org/10.1002/jmv.23638
Espínola EE, et al. Phylogeny-based Classification of Human Rhinoviruses Detected in Hospitalized Children With Acute Lower Respiratory Infection in Paraguay, 2010-2011. J Med Virol. 2013;85(9):1645-51. PubMed PMID: 23780670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phylogeny-based classification of human rhinoviruses detected in hospitalized children with acute lower respiratory infection in Paraguay, 2010-2011. AU - Espínola,Emilio E, AU - Russomando,Graciela, AU - Aquino,Carolina, AU - Basualdo,Wilma, Y1 - 2013/06/18/ PY - 2013/04/04/accepted PY - 2013/6/20/entrez PY - 2013/6/20/pubmed PY - 2014/2/6/medline KW - genotypes KW - respiratory disease KW - rhinovirus SP - 1645 EP - 51 JF - Journal of medical virology JO - J Med Virol VL - 85 IS - 9 N2 - Human rhinovirus (HRV), a single-stranded, positive-sense RNA virus, is associated with mild upper respiratory tract infections in children. The aim of this study was to carry out a molecular characterization and phylogeny-based classification of the circulating genotypes of HRV in hospitalized children with clinical manifestations of acute lower respiratory infection in Paraguay. Nasopharyngeal aspirates were collected from 101 children under 5 years of age, hospitalized with symptoms of acute lower respiratory infection, between May 2010 and December 2011, at the largest public pediatric hospital in the Central Department of Paraguay. Detection was performed by a real-time polymerase chain reaction, followed by conventional amplification of the VP4/VP2 genomic region, sequencing, and phylogenetic analysis. Rhinovirus was detected in 33.7% of the samples. Amplification of 18 samples showed the presence of all three species (HRV-A, -B, and -C). Different genotypes were found for each species: 11 for HRV-A (-9, -12, -22, -30, -36, -43, -59, -61, -68, -88, and -89), one for HRV-B (-4), and four for HRV-C (-C2, -C3, -C6, and -C9). In South America, information about HRV diversity is scarce. This is the first report on HRV genotype diversity in South America. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/23780670/Phylogeny_based_classification_of_human_rhinoviruses_detected_in_hospitalized_children_with_acute_lower_respiratory_infection_in_Paraguay_2010_2011_ L2 - https://doi.org/10.1002/jmv.23638 DB - PRIME DP - Unbound Medicine ER -