Tags

Type your tag names separated by a space and hit enter

Comparative study of peripheral blood smear and quantitative buffy coat in malaria diagnosis.
J Commun Dis. 2011 Mar; 43(1):57-9.JC

Abstract

A rapid test for diagnosis of malaria based on acridine orange staining of centrifuged blood samples in a microhaematocrit tube (QBC) was compared with Leishman stained thin peripheral blood smear in 287 samples. Malaria was diagnosed in 44 patients by Leishman staining technique and in 65 patients by QBC method. The QBC method allowed detection of an additional 21 cases. Thus the prevalence rate of malaria during the study was 22.65%. In 222 Patients who were negative by the QBC technique, the Leishman stained smears were also negative for malarial parasite. Although QBC method was superior to the smear for malarial parasite detection, species identification was difficult by this technique. The QBC method provides a reliable, quick, easily mastered, accurate method for diagnosis of malaria. The QBC system can also be used in the diagnosis of other parasitic diseases from blood (Filariasis). However, Leishman stained thin blood film still appear superior for species identification.

Authors+Show Affiliations

Salmani MP
Department of Microbiology, Shri BM Patil Medical College, Solapur Road, Bijapur -586103, Karnataka. drsalmani@rediffmail.com
Preeti BM
No affiliation info available
Peerapur BV
No affiliation info available

MeSH

AdolescentAdultAgedBlood Buffy CoatChildChild, PreschoolHumansMalaria, FalciparumMalaria, VivaxMiddle AgedSensitivity and SpecificityYoung Adult

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

23785883

Citation

Salmani, Manjunath P., et al. "Comparative Study of Peripheral Blood Smear and Quantitative Buffy Coat in Malaria Diagnosis." The Journal of Communicable Diseases, vol. 43, no. 1, 2011, pp. 57-9.
Salmani MP, Preeti BM, Peerapur BV. Comparative study of peripheral blood smear and quantitative buffy coat in malaria diagnosis. J Commun Dis. 2011;43(1):57-9.
Salmani, M. P., Preeti, B. M., & Peerapur, B. V. (2011). Comparative study of peripheral blood smear and quantitative buffy coat in malaria diagnosis. The Journal of Communicable Diseases, 43(1), 57-9.
Salmani MP, Preeti BM, Peerapur BV. Comparative Study of Peripheral Blood Smear and Quantitative Buffy Coat in Malaria Diagnosis. J Commun Dis. 2011;43(1):57-9. PubMed PMID: 23785883.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative study of peripheral blood smear and quantitative buffy coat in malaria diagnosis. AU - Salmani,Manjunath P, AU - Preeti,B Mindolli, AU - Peerapur,B V, PY - 2013/6/22/entrez PY - 2011/3/1/pubmed PY - 2013/7/13/medline SP - 57 EP - 9 JF - The Journal of communicable diseases JO - J Commun Dis VL - 43 IS - 1 N2 - A rapid test for diagnosis of malaria based on acridine orange staining of centrifuged blood samples in a microhaematocrit tube (QBC) was compared with Leishman stained thin peripheral blood smear in 287 samples. Malaria was diagnosed in 44 patients by Leishman staining technique and in 65 patients by QBC method. The QBC method allowed detection of an additional 21 cases. Thus the prevalence rate of malaria during the study was 22.65%. In 222 Patients who were negative by the QBC technique, the Leishman stained smears were also negative for malarial parasite. Although QBC method was superior to the smear for malarial parasite detection, species identification was difficult by this technique. The QBC method provides a reliable, quick, easily mastered, accurate method for diagnosis of malaria. The QBC system can also be used in the diagnosis of other parasitic diseases from blood (Filariasis). However, Leishman stained thin blood film still appear superior for species identification. SN - 0019-5138 UR - https://www.unboundmedicine.com/medline/citation/23785883/Comparative_study_of_peripheral_blood_smear_and_quantitative_buffy_coat_in_malaria_diagnosis_ L2 - http://www.diseaseinfosearch.org/result/4415 DB - PRIME DP - Unbound Medicine ER -