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[Simultaneous determination of four trace aconitum alkaloids in urine using ultra performance liquid chromatography-mass spectrometry].
Se Pu. 2013 Mar; 31(3):211-7.SP

Abstract

A rapid, specific and sensitive ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous determination of four trace alkaloids, including aconitine (AC), hypaconitine (HA), mesaconitine (MA) and yunaconitine (YA) in human urine samples. UPLC-MS-MS system coupled with an electrospray ionization (ESI) source was performed in multiple reaction monitoring (MRM) mode. The sample preparation was performed with hollow fiber microextraction (HF-LPME) prior to the analysis. The enrichment factors of the four alkaloids were 102 - 301. The separation was applied on a Waters ACQUITY UPLC BEH C18 column (50 mm x 2.1 mm, 1.7 micro m) with a gradient elution of acetonitrile and 10 mmol/L NH4HCO3, as mobile phase. The retention times , were less than 3 min. This method significantly improved the detection sensitivity, and the limits of quantitation were from 0. 01 to 0.1 ng/L. The calibration curves were linear over the ranges of 0.01 - 10 ng/L for AC, MA and YA, 0.1 - 100 ng/L for HA in human urine samples, and the correlation coefficients were 0. 998 1, 0.998 4, 0.999 5 and 0.998 6, respectively. The method was proved to be rapid and sensitive for aconitum alkaloid analysis in urine samples.

Authors+Show Affiliations

School of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23785992

Citation

Zhang, Panpan, et al. "[Simultaneous Determination of Four Trace Aconitum Alkaloids in Urine Using Ultra Performance Liquid Chromatography-mass Spectrometry]." Se Pu = Chinese Journal of Chromatography, vol. 31, no. 3, 2013, pp. 211-7.
Zhang P, Zhang F, Wang Z, et al. [Simultaneous determination of four trace aconitum alkaloids in urine using ultra performance liquid chromatography-mass spectrometry]. Se Pu. 2013;31(3):211-7.
Zhang, P., Zhang, F., Wang, Z., Jiang, Y., & Lu, Y. (2013). [Simultaneous determination of four trace aconitum alkaloids in urine using ultra performance liquid chromatography-mass spectrometry]. Se Pu = Chinese Journal of Chromatography, 31(3), 211-7.
Zhang P, et al. [Simultaneous Determination of Four Trace Aconitum Alkaloids in Urine Using Ultra Performance Liquid Chromatography-mass Spectrometry]. Se Pu. 2013;31(3):211-7. PubMed PMID: 23785992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Simultaneous determination of four trace aconitum alkaloids in urine using ultra performance liquid chromatography-mass spectrometry]. AU - Zhang,Panpan, AU - Zhang,Fucheng, AU - Wang,Zhaohong, AU - Jiang,Ye, AU - Lu,Yongjiang, PY - 2013/6/22/entrez PY - 2013/6/22/pubmed PY - 2014/12/20/medline SP - 211 EP - 7 JF - Se pu = Chinese journal of chromatography JO - Se Pu VL - 31 IS - 3 N2 - A rapid, specific and sensitive ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous determination of four trace alkaloids, including aconitine (AC), hypaconitine (HA), mesaconitine (MA) and yunaconitine (YA) in human urine samples. UPLC-MS-MS system coupled with an electrospray ionization (ESI) source was performed in multiple reaction monitoring (MRM) mode. The sample preparation was performed with hollow fiber microextraction (HF-LPME) prior to the analysis. The enrichment factors of the four alkaloids were 102 - 301. The separation was applied on a Waters ACQUITY UPLC BEH C18 column (50 mm x 2.1 mm, 1.7 micro m) with a gradient elution of acetonitrile and 10 mmol/L NH4HCO3, as mobile phase. The retention times , were less than 3 min. This method significantly improved the detection sensitivity, and the limits of quantitation were from 0. 01 to 0.1 ng/L. The calibration curves were linear over the ranges of 0.01 - 10 ng/L for AC, MA and YA, 0.1 - 100 ng/L for HA in human urine samples, and the correlation coefficients were 0. 998 1, 0.998 4, 0.999 5 and 0.998 6, respectively. The method was proved to be rapid and sensitive for aconitum alkaloid analysis in urine samples. SN - 1000-8713 UR - https://www.unboundmedicine.com/medline/citation/23785992/[Simultaneous_determination_of_four_trace_aconitum_alkaloids_in_urine_using_ultra_performance_liquid_chromatography_mass_spectrometry]_ DB - PRIME DP - Unbound Medicine ER -