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The Shu complex regulates Rad52 localization during rDNA repair.
DNA Repair (Amst). 2013 Sep; 12(9):786-90.DR

Abstract

The Shu complex, consisting of Rad51 paralogues, is an important regulator of homologous recombination, an error-free DNA repair pathway. Consequently, when members of this complex are disrupted, cells exhibit a mutator phenotype, sensitivity to DNA damage reagents and increased gross chromosomal rearrangements. Previously, we found that the Shu complex plays an important role in ribosomal DNA (rDNA) recombination when the Upstream Activating Factor (UAF) protein Uaf30 is disrupted. UAF30 encodes a protein needed for rDNA transcription and when deleted, rDNA recombination increases and the rDNA expands in a Shu1-dependent manner. Here we find using the uaf30-sensitized background that the central DNA repair protein Rad52, which is normally excluded from the nucleolus, frequently overlaps with the rDNA. This close association of Rad52 with the rDNA is dependent upon Shu1 in a uaf30 mutant. Previously, it was shown that in the absence of Rad52 sumoylation, Rad52 foci mislocalize to the nucleolus. Interestingly, here we find that using the uaf30 sensitized background the ability to regulate Rad52 sumoylation is important for Shu1 dependent rDNA recombination as well as Rad52 close association with rDNA. Our results suggest that in the absence of UAF30, the Shu complex plays a central role in Rad52 rDNA localization as long as Rad52 can be sumoylated. This discrimination is important for rDNA copy number homeostasis.

Authors+Show Affiliations

Columbia University Medical Center, Department of Genetics and Development, New York, NY 10032, United States.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

23790361

Citation

Bernstein, Kara A., et al. "The Shu Complex Regulates Rad52 Localization During rDNA Repair." DNA Repair, vol. 12, no. 9, 2013, pp. 786-90.
Bernstein KA, Juanchich A, Sunjevaric I, et al. The Shu complex regulates Rad52 localization during rDNA repair. DNA Repair (Amst). 2013;12(9):786-90.
Bernstein, K. A., Juanchich, A., Sunjevaric, I., & Rothstein, R. (2013). The Shu complex regulates Rad52 localization during rDNA repair. DNA Repair, 12(9), 786-90. https://doi.org/10.1016/j.dnarep.2013.05.003
Bernstein KA, et al. The Shu Complex Regulates Rad52 Localization During rDNA Repair. DNA Repair (Amst). 2013;12(9):786-90. PubMed PMID: 23790361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Shu complex regulates Rad52 localization during rDNA repair. AU - Bernstein,Kara A, AU - Juanchich,Amélie, AU - Sunjevaric,Ivana, AU - Rothstein,Rodney, Y1 - 2013/06/19/ PY - 2013/02/25/received PY - 2013/05/13/revised PY - 2013/05/15/accepted PY - 2013/6/25/entrez PY - 2013/6/25/pubmed PY - 2013/10/19/medline KW - DNA repair KW - Rad52 KW - Rad52 sumoylation KW - Shu complex KW - UAF complex KW - rDNA recombination SP - 786 EP - 90 JF - DNA repair JO - DNA Repair (Amst) VL - 12 IS - 9 N2 - The Shu complex, consisting of Rad51 paralogues, is an important regulator of homologous recombination, an error-free DNA repair pathway. Consequently, when members of this complex are disrupted, cells exhibit a mutator phenotype, sensitivity to DNA damage reagents and increased gross chromosomal rearrangements. Previously, we found that the Shu complex plays an important role in ribosomal DNA (rDNA) recombination when the Upstream Activating Factor (UAF) protein Uaf30 is disrupted. UAF30 encodes a protein needed for rDNA transcription and when deleted, rDNA recombination increases and the rDNA expands in a Shu1-dependent manner. Here we find using the uaf30-sensitized background that the central DNA repair protein Rad52, which is normally excluded from the nucleolus, frequently overlaps with the rDNA. This close association of Rad52 with the rDNA is dependent upon Shu1 in a uaf30 mutant. Previously, it was shown that in the absence of Rad52 sumoylation, Rad52 foci mislocalize to the nucleolus. Interestingly, here we find that using the uaf30 sensitized background the ability to regulate Rad52 sumoylation is important for Shu1 dependent rDNA recombination as well as Rad52 close association with rDNA. Our results suggest that in the absence of UAF30, the Shu complex plays a central role in Rad52 rDNA localization as long as Rad52 can be sumoylated. This discrimination is important for rDNA copy number homeostasis. SN - 1568-7856 UR - https://www.unboundmedicine.com/medline/citation/23790361/The_Shu_complex_regulates_Rad52_localization_during_rDNA_repair_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1568-7864(13)00127-4 DB - PRIME DP - Unbound Medicine ER -