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Multiclass determination of phytochemicals in vegetables and fruits by ultra high performance liquid chromatography coupled to tandem mass spectrometry.
Food Chem. 2013 Nov 15; 141(2):1120-9.FC

Abstract

In this study a simultaneous determination of several classes of phytochemicals (isoflavones, glucosinolates, flavones, flavonols and phenolic acids) in tomato, broccoli, carrot, eggplant and grape has been carried out by ultra high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Solid-liquid extraction assisted by rotary agitator was utilised, using a mixture of methanol:water (80:20, v/v) as solvent. The analytical procedure was validated in all the matrices, obtaining recoveries ranging from 60% to 120% with repeatability values (expressed as relative standard deviations, RSDs) lower than 25%. Limits of quantification (LOQs) were always equal or lower than 50μg/kg, except for some glucosinolates (125μg/kg). Finally the method was applied to different matrices such as tomato, broccoli, carrot, grape and eggplant, observing that chlorogenic acid was detected in most of the samples at higher concentrations in relation to the other compounds.

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Authors+Show Affiliations

Alarcón-Flores MI
Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, ceiA3, E-04120 Almería, Spain.
Romero-González R
No affiliation info available
Vidal JL
No affiliation info available
Frenich AG
No affiliation info available

MeSH

Chromatography, High Pressure LiquidFruitPhytochemicalsPlant ExtractsTandem Mass SpectrometryVegetables

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23790894

Citation

Alarcón-Flores, María Isabel, et al. "Multiclass Determination of Phytochemicals in Vegetables and Fruits By Ultra High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry." Food Chemistry, vol. 141, no. 2, 2013, pp. 1120-9.
Alarcón-Flores MI, Romero-González R, Vidal JL, et al. Multiclass determination of phytochemicals in vegetables and fruits by ultra high performance liquid chromatography coupled to tandem mass spectrometry. Food Chem. 2013;141(2):1120-9.
Alarcón-Flores, M. I., Romero-González, R., Vidal, J. L., & Frenich, A. G. (2013). Multiclass determination of phytochemicals in vegetables and fruits by ultra high performance liquid chromatography coupled to tandem mass spectrometry. Food Chemistry, 141(2), 1120-9. https://doi.org/10.1016/j.foodchem.2013.03.100
Alarcón-Flores MI, et al. Multiclass Determination of Phytochemicals in Vegetables and Fruits By Ultra High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry. Food Chem. 2013 Nov 15;141(2):1120-9. PubMed PMID: 23790894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiclass determination of phytochemicals in vegetables and fruits by ultra high performance liquid chromatography coupled to tandem mass spectrometry. AU - Alarcón-Flores,María Isabel, AU - Romero-González,Roberto, AU - Vidal,José Luis Martínez, AU - Frenich,Antonia Garrido, Y1 - 2013/04/18/ PY - 2012/04/10/received PY - 2013/03/18/revised PY - 2013/03/19/accepted PY - 2013/6/25/entrez PY - 2013/6/26/pubmed PY - 2014/1/5/medline SP - 1120 EP - 9 JF - Food chemistry JO - Food Chem VL - 141 IS - 2 N2 - In this study a simultaneous determination of several classes of phytochemicals (isoflavones, glucosinolates, flavones, flavonols and phenolic acids) in tomato, broccoli, carrot, eggplant and grape has been carried out by ultra high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Solid-liquid extraction assisted by rotary agitator was utilised, using a mixture of methanol:water (80:20, v/v) as solvent. The analytical procedure was validated in all the matrices, obtaining recoveries ranging from 60% to 120% with repeatability values (expressed as relative standard deviations, RSDs) lower than 25%. Limits of quantification (LOQs) were always equal or lower than 50μg/kg, except for some glucosinolates (125μg/kg). Finally the method was applied to different matrices such as tomato, broccoli, carrot, grape and eggplant, observing that chlorogenic acid was detected in most of the samples at higher concentrations in relation to the other compounds. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/23790894/Multiclass_determination_of_phytochemicals_in_vegetables_and_fruits_by_ultra_high_performance_liquid_chromatography_coupled_to_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(13)00445-7 DB - PRIME DP - Unbound Medicine ER -
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