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Experimental challenge of chicken vaccinated with commercially available H5 vaccines reveals loss of protection to some highly pathogenic avian influenza H5N1 strains circulating in Hong Kong/China.
Vaccine 2013; 31(35):3536-42V

Abstract

Highly pathogenic avian influenza (HPAI) H5N1 virus continues to circulate in poultry in Asia and Africa posing a threat to both public and animal health. Vaccination, used as an adjunct to improved bio-security and stamping-out policies, contributed to protecting poultry in Hong Kong from HPAI H5N1 infection in 2004-2008 although the virus was repeatedly detected in dead wild birds. The detection of clade 2.3.4 H5N1 viruses in poultry markets and a farm in Hong Kong in 2008 raised the question whether this virus has changed to evade protection from the H5 vaccines in use. We tested the efficacy of three commercial vaccines (Nobilis, Poulvac and Harbin Re-5 vaccine) in specific pathogen free white leghorn chickens against a challenge with A/chicken/Hong Kong/8825-2/2008 (clade 2.3.4) isolated from vaccinated poultry in Hong Kong and A/chicken/Hong Kong/782/2009 (clade 2.3.2). Harbin Re5 vaccine provided the best, albeit not complete protection against challenge with the clade 2.3.4 virus. All three vaccines provided good protection from death and significantly reduced virus shedding following challenge with the clade 2.3.2 virus. Only Harbin Re-5 was able to completely protect chickens from virus shedding as well as mortality. Sera from vaccinated chickens had lower geometric hemagglutination inhibition titers against A/chicken/Hong Kong/8825-2/08, as compared to two other clade 2.3.4 and one clade 0 virus. Alignment of amino-acid sequences of the haemagglutinin of A/chicken/Hong Kong/8825-2/08 and the other H5 viruses revealed several mutations in positions including 69, 71, 83, 95, 133,140, 162, 183, 189, 194 and 270 (H5 numbering) which may correlate with loss of vaccine protection. Our results indicated that the tested HPAI H5N1 (2.3.4) virus has undergone antigenic changes that allow it to evade immunity from poultry vaccines. This highlights the need for continued surveillance and monitoring of vaccine induced immunity, with experimental vaccine challenge studies being done where indicated.

Authors+Show Affiliations

Center of Influenza Research, School of Public Health and State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23791547

Citation

Connie Leung, Y H., et al. "Experimental Challenge of Chicken Vaccinated With Commercially Available H5 Vaccines Reveals Loss of Protection to some Highly Pathogenic Avian Influenza H5N1 Strains Circulating in Hong Kong/China." Vaccine, vol. 31, no. 35, 2013, pp. 3536-42.
Connie Leung YH, Luk G, Sia SF, et al. Experimental challenge of chicken vaccinated with commercially available H5 vaccines reveals loss of protection to some highly pathogenic avian influenza H5N1 strains circulating in Hong Kong/China. Vaccine. 2013;31(35):3536-42.
Connie Leung, Y. H., Luk, G., Sia, S. F., Wu, Y. O., Ho, C. K., Chow, K. C., ... Malik Peiris, J. S. (2013). Experimental challenge of chicken vaccinated with commercially available H5 vaccines reveals loss of protection to some highly pathogenic avian influenza H5N1 strains circulating in Hong Kong/China. Vaccine, 31(35), pp. 3536-42. doi:10.1016/j.vaccine.2013.05.076.
Connie Leung YH, et al. Experimental Challenge of Chicken Vaccinated With Commercially Available H5 Vaccines Reveals Loss of Protection to some Highly Pathogenic Avian Influenza H5N1 Strains Circulating in Hong Kong/China. Vaccine. 2013 Aug 2;31(35):3536-42. PubMed PMID: 23791547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental challenge of chicken vaccinated with commercially available H5 vaccines reveals loss of protection to some highly pathogenic avian influenza H5N1 strains circulating in Hong Kong/China. AU - Connie Leung,Y H, AU - Luk,Geraldine, AU - Sia,Sin-Fun, AU - Wu,Yu-On, AU - Ho,Chuk-Kwan, AU - Chow,Kai-Chi, AU - Tang,Shing-Chun, AU - Guan,Yi, AU - Malik Peiris,J S, Y1 - 2013/06/19/ PY - 2013/01/16/received PY - 2013/04/01/revised PY - 2013/05/20/accepted PY - 2013/6/25/entrez PY - 2013/6/25/pubmed PY - 2014/4/9/medline KW - Antigenic drift KW - Avian influenza H5N1 KW - Protection KW - Vaccine SP - 3536 EP - 42 JF - Vaccine JO - Vaccine VL - 31 IS - 35 N2 - Highly pathogenic avian influenza (HPAI) H5N1 virus continues to circulate in poultry in Asia and Africa posing a threat to both public and animal health. Vaccination, used as an adjunct to improved bio-security and stamping-out policies, contributed to protecting poultry in Hong Kong from HPAI H5N1 infection in 2004-2008 although the virus was repeatedly detected in dead wild birds. The detection of clade 2.3.4 H5N1 viruses in poultry markets and a farm in Hong Kong in 2008 raised the question whether this virus has changed to evade protection from the H5 vaccines in use. We tested the efficacy of three commercial vaccines (Nobilis, Poulvac and Harbin Re-5 vaccine) in specific pathogen free white leghorn chickens against a challenge with A/chicken/Hong Kong/8825-2/2008 (clade 2.3.4) isolated from vaccinated poultry in Hong Kong and A/chicken/Hong Kong/782/2009 (clade 2.3.2). Harbin Re5 vaccine provided the best, albeit not complete protection against challenge with the clade 2.3.4 virus. All three vaccines provided good protection from death and significantly reduced virus shedding following challenge with the clade 2.3.2 virus. Only Harbin Re-5 was able to completely protect chickens from virus shedding as well as mortality. Sera from vaccinated chickens had lower geometric hemagglutination inhibition titers against A/chicken/Hong Kong/8825-2/08, as compared to two other clade 2.3.4 and one clade 0 virus. Alignment of amino-acid sequences of the haemagglutinin of A/chicken/Hong Kong/8825-2/08 and the other H5 viruses revealed several mutations in positions including 69, 71, 83, 95, 133,140, 162, 183, 189, 194 and 270 (H5 numbering) which may correlate with loss of vaccine protection. Our results indicated that the tested HPAI H5N1 (2.3.4) virus has undergone antigenic changes that allow it to evade immunity from poultry vaccines. This highlights the need for continued surveillance and monitoring of vaccine induced immunity, with experimental vaccine challenge studies being done where indicated. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/23791547/Experimental_challenge_of_chicken_vaccinated_with_commercially_available_H5_vaccines_reveals_loss_of_protection_to_some_highly_pathogenic_avian_influenza_H5N1_strains_circulating_in_Hong_Kong/China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(13)00691-9 DB - PRIME DP - Unbound Medicine ER -