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Fisetin, a tetra hydroxy flavone recuperates antioxidant status and protects hepatocellular ultrastructure from hyperglycemia mediated oxidative stress in streptozotocin induced experimental diabetes in rats.
Food Chem Toxicol. 2013 Sep; 59:249-55.FC

Abstract

Oxidative stress is a biological entity quoted as accountable for several pathological conditions including diabetes mellitus. Chronic hyperglycemia in diabetes is associated with oxidative stress mediated tissue damage. The present study is aimed to explore the role of fisetin, in ameliorating hyperglycemia-mediated oxidative damage to liver in streptozotocin induced diabetic rats. In addition to the levels of blood glucose, plasma insulin, glycosylated hemoglobin, the extent of oxidative stress was assessed by hepatic lipid peroxides and hydroperoxides. The levels of reduced glutathione and the activities of enzymatic antioxidants were determined in the liver tissues. The activities of serum aminotransferases and alkaline phosphatase were assayed. A portion of liver was processed for histological and ultrastructural studies. Oral administration of fisetin (10 mg/kg b. w.) to diabetic rats decreased the levels of blood glucose and glycosylated hemoglobin and increased the plasma insulin level. A reduction in lipid peroxides and hydroperoxides were observed. The diminished activities of antioxidant enzymes and reduced glutathione in diabetic rats were improved upon fisetin administration. Thus, the results of the present study indicate that fisetin treatment protects the hepatocytes by improving the antioxidant competence in hepatic tissues of diabetic rats which is further evidenced from histological and ultra structural observations.

Authors+Show Affiliations

Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23791753

Citation

Prasath, Gopalan Sriram, and Sorimuthu Pillai Subramanian. "Fisetin, a Tetra Hydroxy Flavone Recuperates Antioxidant Status and Protects Hepatocellular Ultrastructure From Hyperglycemia Mediated Oxidative Stress in Streptozotocin Induced Experimental Diabetes in Rats." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 59, 2013, pp. 249-55.
Prasath GS, Subramanian SP. Fisetin, a tetra hydroxy flavone recuperates antioxidant status and protects hepatocellular ultrastructure from hyperglycemia mediated oxidative stress in streptozotocin induced experimental diabetes in rats. Food Chem Toxicol. 2013;59:249-55.
Prasath, G. S., & Subramanian, S. P. (2013). Fisetin, a tetra hydroxy flavone recuperates antioxidant status and protects hepatocellular ultrastructure from hyperglycemia mediated oxidative stress in streptozotocin induced experimental diabetes in rats. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 59, 249-55. https://doi.org/10.1016/j.fct.2013.05.062
Prasath GS, Subramanian SP. Fisetin, a Tetra Hydroxy Flavone Recuperates Antioxidant Status and Protects Hepatocellular Ultrastructure From Hyperglycemia Mediated Oxidative Stress in Streptozotocin Induced Experimental Diabetes in Rats. Food Chem Toxicol. 2013;59:249-55. PubMed PMID: 23791753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fisetin, a tetra hydroxy flavone recuperates antioxidant status and protects hepatocellular ultrastructure from hyperglycemia mediated oxidative stress in streptozotocin induced experimental diabetes in rats. AU - Prasath,Gopalan Sriram, AU - Subramanian,Sorimuthu Pillai, Y1 - 2013/06/19/ PY - 2013/02/19/received PY - 2013/05/29/revised PY - 2013/05/30/accepted PY - 2013/6/25/entrez PY - 2013/6/25/pubmed PY - 2014/3/29/medline KW - Antioxidant KW - CAT KW - DMSO KW - Fisetin KW - GPx KW - GR KW - GSH KW - GST KW - HbAlc KW - Hepatocytes KW - Hyperglycemia KW - LSD KW - Oxidative stress KW - ROS KW - SD KW - SOD KW - STZ KW - TBARS KW - Tissue protective nature KW - catalase KW - dimethyl sulphoxide KW - glutathione S transferase KW - glutathione peroxidase KW - glutathione reductase KW - glycosylated hemoglobin KW - least significant difference KW - reactive oxygen species KW - reduced glutathione KW - standard deviation KW - streptozotocin KW - superoxide dismutase KW - thiobarbituric acid reactive substances SP - 249 EP - 55 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 59 N2 - Oxidative stress is a biological entity quoted as accountable for several pathological conditions including diabetes mellitus. Chronic hyperglycemia in diabetes is associated with oxidative stress mediated tissue damage. The present study is aimed to explore the role of fisetin, in ameliorating hyperglycemia-mediated oxidative damage to liver in streptozotocin induced diabetic rats. In addition to the levels of blood glucose, plasma insulin, glycosylated hemoglobin, the extent of oxidative stress was assessed by hepatic lipid peroxides and hydroperoxides. The levels of reduced glutathione and the activities of enzymatic antioxidants were determined in the liver tissues. The activities of serum aminotransferases and alkaline phosphatase were assayed. A portion of liver was processed for histological and ultrastructural studies. Oral administration of fisetin (10 mg/kg b. w.) to diabetic rats decreased the levels of blood glucose and glycosylated hemoglobin and increased the plasma insulin level. A reduction in lipid peroxides and hydroperoxides were observed. The diminished activities of antioxidant enzymes and reduced glutathione in diabetic rats were improved upon fisetin administration. Thus, the results of the present study indicate that fisetin treatment protects the hepatocytes by improving the antioxidant competence in hepatic tissues of diabetic rats which is further evidenced from histological and ultra structural observations. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/23791753/Fisetin_a_tetra_hydroxy_flavone_recuperates_antioxidant_status_and_protects_hepatocellular_ultrastructure_from_hyperglycemia_mediated_oxidative_stress_in_streptozotocin_induced_experimental_diabetes_in_rats_ DB - PRIME DP - Unbound Medicine ER -