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Neuraminidase inhibitor susceptibility profile of pandemic and seasonal influenza viruses during the 2009-2010 and 2010-2011 influenza seasons in Japan.
Antiviral Res 2013; 99(3):261-9AR

Abstract

Two new influenza virus neuraminidase inhibitors (NAIs), peramivir and laninamivir, were approved in 2010 which resulted to four NAIs that were used during the 2010-2011 influenza season in Japan. This study aims to monitor the susceptibility of influenza virus isolates in 2009-2010 and 2010-2011 influenza seasons in Japan to the four NAIs using the fluorescence-based 50% inhibitory concentration (IC₅₀) method. Outliers were identified using box-and-whisker plot analysis and full NA gene sequencing was performed to determine the mutations that are associated with reduction of susceptibility to NAIs. A total of 117 influenza A(H1N1)pdm09, 59 A(H3N2), and 18 type B viruses were tested before NAI treatment and eight A(H1N1)pdm09 and 1 type B viruses were examined from patients after NAI treatment in the two seasons. NA inhibition assay showed type A influenza viruses were more susceptible to NAIs than type B viruses. The peramivir and laninamivir IC₅₀ values of both type A and B viruses were significantly lower than the oseltamivir and zanamivir IC₅₀ values. Among influenza A(H1N1)pdm09 viruses, the prevalence of H274Y viruses increased from 0% in the 2009-2010 season to 3% in the 2010-2011 season. These H274Y viruses were resistant to oseltamivir and peramivir with 200-300 fold increase in IC₅₀ values but remained sensitive to zanamivir and laninamivir. Other mutations in NA, such as I222T and M241I were identified among the outliers. Among influenza A(H3N2) viruses, two outliers were identified with D151G and T148I mutations, which exhibited a reduction in susceptibility to oseltamivir and zanamivir, respectively. Among type B viruses, no outliers were identified to the four NAIs. For paired samples that were collected before and after drug treatment, three (3/11; 27.3%) H274Y viruses were identified among A(H1N1)pdm09 viruses after oseltamivir treatment but no outliers were found in the laninamivir-treatment group (n=3). Despite widespread use of NAIs in Japan, the prevalence of NAI-resistant influenza viruses is still low.

Authors+Show Affiliations

Division of International Health (Public Health), Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan. Electronic address: clyde@med.niigata-u.ac.jp.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23791870

Citation

Dapat, Clyde, et al. "Neuraminidase Inhibitor Susceptibility Profile of Pandemic and Seasonal Influenza Viruses During the 2009-2010 and 2010-2011 Influenza Seasons in Japan." Antiviral Research, vol. 99, no. 3, 2013, pp. 261-9.
Dapat C, Kondo H, Dapat IC, et al. Neuraminidase inhibitor susceptibility profile of pandemic and seasonal influenza viruses during the 2009-2010 and 2010-2011 influenza seasons in Japan. Antiviral Res. 2013;99(3):261-9.
Dapat, C., Kondo, H., Dapat, I. C., Baranovich, T., Suzuki, Y., Shobugawa, Y., ... Suzuki, H. (2013). Neuraminidase inhibitor susceptibility profile of pandemic and seasonal influenza viruses during the 2009-2010 and 2010-2011 influenza seasons in Japan. Antiviral Research, 99(3), pp. 261-9. doi:10.1016/j.antiviral.2013.06.003.
Dapat C, et al. Neuraminidase Inhibitor Susceptibility Profile of Pandemic and Seasonal Influenza Viruses During the 2009-2010 and 2010-2011 Influenza Seasons in Japan. Antiviral Res. 2013;99(3):261-9. PubMed PMID: 23791870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuraminidase inhibitor susceptibility profile of pandemic and seasonal influenza viruses during the 2009-2010 and 2010-2011 influenza seasons in Japan. AU - Dapat,Clyde, AU - Kondo,Hiroki, AU - Dapat,Isolde C, AU - Baranovich,Tatiana, AU - Suzuki,Yasushi, AU - Shobugawa,Yugo, AU - Saito,Kousuke, AU - Saito,Reiko, AU - Suzuki,Hiroshi, Y1 - 2013/06/17/ PY - 2012/12/21/received PY - 2013/06/05/revised PY - 2013/06/09/accepted PY - 2013/6/25/entrez PY - 2013/6/26/pubmed PY - 2014/5/3/medline KW - Half maximal inhibitory concentration (IC50) KW - Influenza KW - Laninamivir KW - Oseltamivir KW - Peramivir KW - Zanamivir SP - 261 EP - 9 JF - Antiviral research JO - Antiviral Res. VL - 99 IS - 3 N2 - Two new influenza virus neuraminidase inhibitors (NAIs), peramivir and laninamivir, were approved in 2010 which resulted to four NAIs that were used during the 2010-2011 influenza season in Japan. This study aims to monitor the susceptibility of influenza virus isolates in 2009-2010 and 2010-2011 influenza seasons in Japan to the four NAIs using the fluorescence-based 50% inhibitory concentration (IC₅₀) method. Outliers were identified using box-and-whisker plot analysis and full NA gene sequencing was performed to determine the mutations that are associated with reduction of susceptibility to NAIs. A total of 117 influenza A(H1N1)pdm09, 59 A(H3N2), and 18 type B viruses were tested before NAI treatment and eight A(H1N1)pdm09 and 1 type B viruses were examined from patients after NAI treatment in the two seasons. NA inhibition assay showed type A influenza viruses were more susceptible to NAIs than type B viruses. The peramivir and laninamivir IC₅₀ values of both type A and B viruses were significantly lower than the oseltamivir and zanamivir IC₅₀ values. Among influenza A(H1N1)pdm09 viruses, the prevalence of H274Y viruses increased from 0% in the 2009-2010 season to 3% in the 2010-2011 season. These H274Y viruses were resistant to oseltamivir and peramivir with 200-300 fold increase in IC₅₀ values but remained sensitive to zanamivir and laninamivir. Other mutations in NA, such as I222T and M241I were identified among the outliers. Among influenza A(H3N2) viruses, two outliers were identified with D151G and T148I mutations, which exhibited a reduction in susceptibility to oseltamivir and zanamivir, respectively. Among type B viruses, no outliers were identified to the four NAIs. For paired samples that were collected before and after drug treatment, three (3/11; 27.3%) H274Y viruses were identified among A(H1N1)pdm09 viruses after oseltamivir treatment but no outliers were found in the laninamivir-treatment group (n=3). Despite widespread use of NAIs in Japan, the prevalence of NAI-resistant influenza viruses is still low. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/23791870/Neuraminidase_inhibitor_susceptibility_profile_of_pandemic_and_seasonal_influenza_viruses_during_the_2009_2010_and_2010_2011_influenza_seasons_in_Japan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-3542(13)00160-5 DB - PRIME DP - Unbound Medicine ER -