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Sex steroid receptor expression and localization in benign prostatic hyperplasia varies with tissue compartment.
Differentiation 2013 Apr-Jun; 85(4-5):140-9D

Abstract

Androgens and estrogens, acting via their respective receptors, are important in benign prostatic hyperplasia (BPH). The goals of this study were to quantitatively characterize the tissue distribution and staining intensity of androgen receptor (AR) and estrogen receptor-alpha (ERα), and assess cells expressing both AR and ERα, in human BPH compared to normal prostate. A tissue microarray composed of normal prostate and BPH tissue was used and multiplexed immunohistochemistry was performed to detect AR and ERα. We used a multispectral imaging platform for automated scanning, tissue and cell segmentation and marker quantification. BPH specimens had an increased number of epithelial and stromal cells and increased percentage of epithelium. In both stroma and epithelium, the mean nuclear area was decreased in BPH relative to normal prostate. AR expression and staining intensity in epithelial and stromal cells was significantly increased in BPH compared to normal prostate. ERα expression was increased in BPH epithelium. However, stromal ERα expression and staining intensity was decreased in BPH compared to normal prostate. Double positive (AR and ERα) epithelial cells were more prevalent in BPH, and fewer double negative (AR and ERα) stromal and epithelial negative cells were observed in BPH. These data underscore the importance of tissue layer localization and expression of steroid hormone receptors in the prostate. Understanding the tissue-specific hormone action of androgens and estrogens will lead to a better understanding of mechanisms of pathogenesis in the prostate and may lead to better treatment for BPH.

Authors+Show Affiliations

Department of Urology, University of Wisconsin, Madison, WI, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

23792768

Citation

Nicholson, Tristan M., et al. "Sex Steroid Receptor Expression and Localization in Benign Prostatic Hyperplasia Varies With Tissue Compartment." Differentiation; Research in Biological Diversity, vol. 85, no. 4-5, 2013, pp. 140-9.
Nicholson TM, Sehgal PD, Drew SA, et al. Sex steroid receptor expression and localization in benign prostatic hyperplasia varies with tissue compartment. Differentiation. 2013;85(4-5):140-9.
Nicholson, T. M., Sehgal, P. D., Drew, S. A., Huang, W., & Ricke, W. A. (2013). Sex steroid receptor expression and localization in benign prostatic hyperplasia varies with tissue compartment. Differentiation; Research in Biological Diversity, 85(4-5), pp. 140-9. doi:10.1016/j.diff.2013.02.006.
Nicholson TM, et al. Sex Steroid Receptor Expression and Localization in Benign Prostatic Hyperplasia Varies With Tissue Compartment. Differentiation. 2013;85(4-5):140-9. PubMed PMID: 23792768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sex steroid receptor expression and localization in benign prostatic hyperplasia varies with tissue compartment. AU - Nicholson,Tristan M, AU - Sehgal,Priyanka D, AU - Drew,Sally A, AU - Huang,Wei, AU - Ricke,William A, Y1 - 2013/06/20/ PY - 2012/12/17/received PY - 2013/02/16/revised PY - 2013/02/27/accepted PY - 2013/6/25/entrez PY - 2013/6/25/pubmed PY - 2013/11/10/medline KW - 5-alpha reductase inhibitors KW - 5ARI KW - ACTA2 KW - AR KW - Androgen receptor KW - BPH KW - Benign prostatic hyperplasia KW - DAB KW - ERα KW - Estrogen receptor-alpha KW - HT KW - IHC KW - LUTS KW - PSA KW - SERMs KW - TMA KW - TURP KW - androgen receptor KW - benign prostatic hyperplasia KW - d-amino benzene KW - estrogen receptor-alpha KW - hematoxylin KW - immunohistochemistry KW - lower urinary tract symptoms KW - prostate specific antigen KW - selective estrogen receptor modulators KW - smooth muscle alpha-actin KW - tissue microarray KW - transurethral resection of the prostate SP - 140 EP - 9 JF - Differentiation; research in biological diversity JO - Differentiation VL - 85 IS - 4-5 N2 - Androgens and estrogens, acting via their respective receptors, are important in benign prostatic hyperplasia (BPH). The goals of this study were to quantitatively characterize the tissue distribution and staining intensity of androgen receptor (AR) and estrogen receptor-alpha (ERα), and assess cells expressing both AR and ERα, in human BPH compared to normal prostate. A tissue microarray composed of normal prostate and BPH tissue was used and multiplexed immunohistochemistry was performed to detect AR and ERα. We used a multispectral imaging platform for automated scanning, tissue and cell segmentation and marker quantification. BPH specimens had an increased number of epithelial and stromal cells and increased percentage of epithelium. In both stroma and epithelium, the mean nuclear area was decreased in BPH relative to normal prostate. AR expression and staining intensity in epithelial and stromal cells was significantly increased in BPH compared to normal prostate. ERα expression was increased in BPH epithelium. However, stromal ERα expression and staining intensity was decreased in BPH compared to normal prostate. Double positive (AR and ERα) epithelial cells were more prevalent in BPH, and fewer double negative (AR and ERα) stromal and epithelial negative cells were observed in BPH. These data underscore the importance of tissue layer localization and expression of steroid hormone receptors in the prostate. Understanding the tissue-specific hormone action of androgens and estrogens will lead to a better understanding of mechanisms of pathogenesis in the prostate and may lead to better treatment for BPH. SN - 1432-0436 UR - https://www.unboundmedicine.com/medline/citation/23792768/Sex_steroid_receptor_expression_and_localization_in_benign_prostatic_hyperplasia_varies_with_tissue_compartment_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0301-4681(13)00018-2 DB - PRIME DP - Unbound Medicine ER -