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Molecular characterization of hepatitis A virus isolated from acute infections in Turkey.
Turk J Gastroenterol. 2012; 23(6):714-9.TJ

Abstract

BACKGROUND/AIMS

Hepatitis A virus is a global public health problem, especially in developing countries, and the most common cause of hepatitis in childhood. Hepatitis A virus is a single- stranded positive RNA virus subdivided to 6 genotypes (3 human,3 simian). The aim of this study was to determine the prevalent genotype in Turkey using sera of acute hepatitis A virus-infected patients from different geographical regions of the country.

MATERIALS AND METHODS

Sera of 137 patients with acute hepatitis A virus from different geographical regions were collected for phylogenetic analysis. The VP1-2A region of the hepatitis A virus genome was amplified by real-time-polymerase chain reaction in 76 patients where possible. Amplified polymerase chain reaction fragments were sequenced, and phylogenetic analysis was done together with other reference hepatitis A virus sequences obtained from GenBank database.

RESULTS

Sequencing and phylogenetic analysis of the VP1-2A junction of hepatitis A virus showed that the most prevalent genotype in Turkey is IB (100%). Comparison of Turkish isolates and reference sequences of genotype IB showed a similarity of 94.9%. The same comparison was done between Turkish isolates and reference hepatitis A virus genotype IB and HM175, and it was found that similarity between them ranged from 93.0-95.9%. When Turkish isolates were compared according to Mean Percentage Nucleotide Distance analysis, similarity ranged between 95.3%-100%.

CONCLUSIONS

Phylogenetic analysis pointed out that all Turkish isolates belong to genotype IB. Sequence analysis is a useful tool in revealing hepatitis A outbreaks, and allows us to detect and distinguish the presence of epidemic and small outbreaks.

Authors+Show Affiliations

Department of Medical Microbiology, Turkey Yüksek İhtisas Hospital, Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23794310

Citation

Dınç, Bedia, et al. "Molecular Characterization of Hepatitis a Virus Isolated From Acute Infections in Turkey." The Turkish Journal of Gastroenterology : the Official Journal of Turkish Society of Gastroenterology, vol. 23, no. 6, 2012, pp. 714-9.
Dınç B, Koyuncu D, Karatayli SC, et al. Molecular characterization of hepatitis A virus isolated from acute infections in Turkey. Turk J Gastroenterol. 2012;23(6):714-9.
Dınç, B., Koyuncu, D., Karatayli, S. C., Berk, E., Karatayli, E., Parlak, M., Çelık, I., Akgüç, M., Sertöz, R., Berktaş, M., Bozdayi, G., & Bozdayi, A. M. (2012). Molecular characterization of hepatitis A virus isolated from acute infections in Turkey. The Turkish Journal of Gastroenterology : the Official Journal of Turkish Society of Gastroenterology, 23(6), 714-9.
Dınç B, et al. Molecular Characterization of Hepatitis a Virus Isolated From Acute Infections in Turkey. Turk J Gastroenterol. 2012;23(6):714-9. PubMed PMID: 23794310.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of hepatitis A virus isolated from acute infections in Turkey. AU - Dınç,Bedia, AU - Koyuncu,Duygu, AU - Karatayli,Senem Ceren, AU - Berk,Elife, AU - Karatayli,Ersin, AU - Parlak,Mehmet, AU - Çelık,Inci, AU - Akgüç,Miray, AU - Sertöz,Rüçhan, AU - Berktaş,Mustafa, AU - Bozdayi,Gülendam, AU - Bozdayi,A Mithat, PY - 2013/6/25/entrez PY - 2013/6/26/pubmed PY - 2014/2/5/medline SP - 714 EP - 9 JF - The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology JO - Turk J Gastroenterol VL - 23 IS - 6 N2 - BACKGROUND/AIMS: Hepatitis A virus is a global public health problem, especially in developing countries, and the most common cause of hepatitis in childhood. Hepatitis A virus is a single- stranded positive RNA virus subdivided to 6 genotypes (3 human,3 simian). The aim of this study was to determine the prevalent genotype in Turkey using sera of acute hepatitis A virus-infected patients from different geographical regions of the country. MATERIALS AND METHODS: Sera of 137 patients with acute hepatitis A virus from different geographical regions were collected for phylogenetic analysis. The VP1-2A region of the hepatitis A virus genome was amplified by real-time-polymerase chain reaction in 76 patients where possible. Amplified polymerase chain reaction fragments were sequenced, and phylogenetic analysis was done together with other reference hepatitis A virus sequences obtained from GenBank database. RESULTS: Sequencing and phylogenetic analysis of the VP1-2A junction of hepatitis A virus showed that the most prevalent genotype in Turkey is IB (100%). Comparison of Turkish isolates and reference sequences of genotype IB showed a similarity of 94.9%. The same comparison was done between Turkish isolates and reference hepatitis A virus genotype IB and HM175, and it was found that similarity between them ranged from 93.0-95.9%. When Turkish isolates were compared according to Mean Percentage Nucleotide Distance analysis, similarity ranged between 95.3%-100%. CONCLUSIONS: Phylogenetic analysis pointed out that all Turkish isolates belong to genotype IB. Sequence analysis is a useful tool in revealing hepatitis A outbreaks, and allows us to detect and distinguish the presence of epidemic and small outbreaks. SN - 2148-5607 UR - https://www.unboundmedicine.com/medline/citation/23794310/Molecular_characterization_of_hepatitis_A_virus_isolated_from_acute_infections_in_Turkey_ L2 - http://www.turkjgastroenterol.org/eng/makale/2938/208/Full-Text DB - PRIME DP - Unbound Medicine ER -