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Synergistic effect of black tea and curcumin in improving the hepatotoxicity induced by aflatoxin B1 in rats.

Abstract

Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carcinogenic due its potential in inducing the oxidative stress and distortion of the most antioxidant enzymes. Since black tea possesses strong antioxidant activity, it protects cells and tissues against oxidative stress. Curcumin (CMN), a naturally occurring agent, has a combination of biological and pharmacological properties that include antioxidant activity. Therefore, the present study was carried out to investigate the possible role of separate and mixed supplementation of black tea extract and CMN in the hepatotoxicity induced by AFB1 in rats. A total of 48: adult male Sprague Dawley rats were randomly divided into eight groups with six rats in each group. Group 1 (normal control) includes rats that received no treatment. Groups 2, 3, and 4 (positive control) include rats that received olive oil, black tea extract, and CMN, respectively. Group 5 includes rats that received AFB1 at a dose of 750 μg/kg body weight (b.w.) dissolved in olive oil. Groups 6, 7, and 8 include rats that received AFB1 along with 2% black tea extract, CMN at a dose of 200 mg/kg b.w., and both black tea extract and CMN at the same previous doses, respectively. After 90 days, biochemical and histopathological examination was carried out for the blood samples and liver tissues. A significant decrease in the antioxidant enzymes and a significant increase in the lipid peroxidation and hydrogen peroxide in the rats treated with AFB1 were observed. Moreover, there were dramatic changes in the liver function biomarkers, lipid profile, and liver architecture. Supplementation of black tea extract or CMN showed an efficient role in repairing the distortion of the biochemical and histological changes induced by AFB1 in liver. This improvement was more pronounced when both CMN and black tea were used together.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt abeer75875@hotmail.com.

    ,

    Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.

    ,

    Department of Biology, Faculty of Science, King Khalid University, Abha, Saudi Arabia.

    Department of Biology, Faculty of Science, King Khalid University, Abha, Saudi Arabia.

    Source

    Toxicology and industrial health 31:12 2015 Dec pg 1269-80

    MeSH

    Aflatoxin B1
    Animals
    Antioxidants
    Biomarkers
    Camellia sinensis
    Curcumin
    Dietary Supplements
    Food Handling
    Hepatic Insufficiency
    Lipid Metabolism
    Lipid Peroxidation
    Liver
    Male
    Oxidation-Reduction
    Oxidative Stress
    Plant Extracts
    Plant Leaves
    Random Allocation
    Rats, Sprague-Dawley
    Tea

    Pub Type(s)

    Comparative Study
    Journal Article

    Language

    eng

    PubMed ID

    23796760

    Citation

    TY - JOUR T1 - Synergistic effect of black tea and curcumin in improving the hepatotoxicity induced by aflatoxin B1 in rats. AU - Alm-Eldeen,Abeer A, AU - Mona,Mohamed H, AU - Shati,Ali A, AU - El-Mekkawy,Haitham I, Y1 - 2013/06/24/ PY - 2013/6/26/entrez PY - 2013/6/26/pubmed PY - 2016/9/17/medline KW - Aflatoxin B1 KW - black tea KW - curcumin KW - histopathology KW - liver KW - oxidative stress KW - rat SP - 1269 EP - 80 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 31 IS - 12 N2 - Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carcinogenic due its potential in inducing the oxidative stress and distortion of the most antioxidant enzymes. Since black tea possesses strong antioxidant activity, it protects cells and tissues against oxidative stress. Curcumin (CMN), a naturally occurring agent, has a combination of biological and pharmacological properties that include antioxidant activity. Therefore, the present study was carried out to investigate the possible role of separate and mixed supplementation of black tea extract and CMN in the hepatotoxicity induced by AFB1 in rats. A total of 48: adult male Sprague Dawley rats were randomly divided into eight groups with six rats in each group. Group 1 (normal control) includes rats that received no treatment. Groups 2, 3, and 4 (positive control) include rats that received olive oil, black tea extract, and CMN, respectively. Group 5 includes rats that received AFB1 at a dose of 750 μg/kg body weight (b.w.) dissolved in olive oil. Groups 6, 7, and 8 include rats that received AFB1 along with 2% black tea extract, CMN at a dose of 200 mg/kg b.w., and both black tea extract and CMN at the same previous doses, respectively. After 90 days, biochemical and histopathological examination was carried out for the blood samples and liver tissues. A significant decrease in the antioxidant enzymes and a significant increase in the lipid peroxidation and hydrogen peroxide in the rats treated with AFB1 were observed. Moreover, there were dramatic changes in the liver function biomarkers, lipid profile, and liver architecture. Supplementation of black tea extract or CMN showed an efficient role in repairing the distortion of the biochemical and histological changes induced by AFB1 in liver. This improvement was more pronounced when both CMN and black tea were used together. SN - 1477-0393 UR - https://www.unboundmedicine.com/medline/citation/23796760/Synergistic_effect_of_black_tea_and_curcumin_in_improving_the_hepatotoxicity_induced_by_aflatoxin_B1_in_rats_ L2 - http://journals.sagepub.com/doi/abs/10.1177/0748233713491807?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed ER -