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Silver nanoparticles induce endoplasmatic reticulum stress response in zebrafish.
Toxicol Appl Pharmacol. 2013 Oct 15; 272(2):519-28.TA

Abstract

Silver nanoparticles (AgNPs) find increasing applications, and therefore humans and the environment are increasingly exposed to them. However, potential toxicological implications are not sufficiently known. Here we investigate effects of AgNPs (average size 120 nm) on zebrafish in vitro and in vivo, and compare them to human hepatoma cells (Huh7). AgNPs are incorporated in zebrafish liver cells (ZFL) and Huh7, and in zebrafish embryos. In ZFL cells AgNPs lead to induction of reactive oxygen species (ROS), endoplasmatic reticulum (ER) stress response, and TNF-α. Transcriptional alterations also occur in pro-apoptotic genes p53 and Bax. The transcriptional profile differed in ZFL and Huh7 cells. In ZFL cells, the ER stress marker BiP is induced, concomitant with the ER stress marker ATF-6 and spliced XBP-1 after 6h and 24h exposure to 0.5 g/L and 0.05 g/L AgNPs, respectively. This indicates the induction of different pathways of the ER stress response. Moreover, AgNPs induce TNF-α. In zebrafish embryos exposed to 0.01, 0.1, 1 and 5mg/L AgNPs hatching was affected and morphological defects occurred at high concentrations. ER stress related gene transcripts BiP and Synv are significantly up-regulated after 24h at 0.1 and 5mg/L AgNPs. Furthermore, transcriptional alterations occurred in the pro-apoptotic genes Noxa and p21. The ER stress response was strong in ZFL cells and occurred in zebrafish embryos as well. Our data demonstrate for the first time that AgNPs lead to induction of ER stress in zebrafish. The induction of ER stress can have several consequences including the activation of apoptotic and inflammatory pathways.

Authors+Show Affiliations

University of Applied Sciences and Arts Northwestern Switzerland, School of Life Sciences, Gründenstrasse 40, CH-4132 Muttenz, Switzerland.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

23800688

Citation

Christen, Verena, et al. "Silver Nanoparticles Induce Endoplasmatic Reticulum Stress Response in Zebrafish." Toxicology and Applied Pharmacology, vol. 272, no. 2, 2013, pp. 519-28.
Christen V, Capelle M, Fent K. Silver nanoparticles induce endoplasmatic reticulum stress response in zebrafish. Toxicol Appl Pharmacol. 2013;272(2):519-28.
Christen, V., Capelle, M., & Fent, K. (2013). Silver nanoparticles induce endoplasmatic reticulum stress response in zebrafish. Toxicology and Applied Pharmacology, 272(2), 519-28. https://doi.org/10.1016/j.taap.2013.06.011
Christen V, Capelle M, Fent K. Silver Nanoparticles Induce Endoplasmatic Reticulum Stress Response in Zebrafish. Toxicol Appl Pharmacol. 2013 Oct 15;272(2):519-28. PubMed PMID: 23800688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanoparticles induce endoplasmatic reticulum stress response in zebrafish. AU - Christen,Verena, AU - Capelle,Martinus, AU - Fent,Karl, Y1 - 2013/06/22/ PY - 2013/04/11/received PY - 2013/06/01/revised PY - 2013/06/13/accepted PY - 2013/6/27/entrez PY - 2013/6/27/pubmed PY - 2013/12/16/medline KW - ER stress response KW - Nanoparticles KW - Silver nanoparticles KW - Toxicity KW - Zebrafish SP - 519 EP - 28 JF - Toxicology and applied pharmacology JO - Toxicol Appl Pharmacol VL - 272 IS - 2 N2 - Silver nanoparticles (AgNPs) find increasing applications, and therefore humans and the environment are increasingly exposed to them. However, potential toxicological implications are not sufficiently known. Here we investigate effects of AgNPs (average size 120 nm) on zebrafish in vitro and in vivo, and compare them to human hepatoma cells (Huh7). AgNPs are incorporated in zebrafish liver cells (ZFL) and Huh7, and in zebrafish embryos. In ZFL cells AgNPs lead to induction of reactive oxygen species (ROS), endoplasmatic reticulum (ER) stress response, and TNF-α. Transcriptional alterations also occur in pro-apoptotic genes p53 and Bax. The transcriptional profile differed in ZFL and Huh7 cells. In ZFL cells, the ER stress marker BiP is induced, concomitant with the ER stress marker ATF-6 and spliced XBP-1 after 6h and 24h exposure to 0.5 g/L and 0.05 g/L AgNPs, respectively. This indicates the induction of different pathways of the ER stress response. Moreover, AgNPs induce TNF-α. In zebrafish embryos exposed to 0.01, 0.1, 1 and 5mg/L AgNPs hatching was affected and morphological defects occurred at high concentrations. ER stress related gene transcripts BiP and Synv are significantly up-regulated after 24h at 0.1 and 5mg/L AgNPs. Furthermore, transcriptional alterations occurred in the pro-apoptotic genes Noxa and p21. The ER stress response was strong in ZFL cells and occurred in zebrafish embryos as well. Our data demonstrate for the first time that AgNPs lead to induction of ER stress in zebrafish. The induction of ER stress can have several consequences including the activation of apoptotic and inflammatory pathways. SN - 1096-0333 UR - https://www.unboundmedicine.com/medline/citation/23800688/Silver_nanoparticles_induce_endoplasmatic_reticulum_stress_response_in_zebrafish_ DB - PRIME DP - Unbound Medicine ER -