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Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS.
RNA. 2013 Aug; 19(8):1089-104.RNA

Abstract

OxyS and RprA are two small noncoding RNAs (sRNAs) that modulate the expression of rpoS, encoding an alternative sigma factor that activates transcription of multiple Escherichia coli stress-response genes. While RprA activates rpoS for translation, OxyS down-regulates the transcript. Crucially, the RNA binding protein Hfq is required for both sRNAs to function, although the specific role played by Hfq remains unclear. We have investigated RprA and OxyS interactions with Hfq using biochemical and biophysical approaches. In particular, we have obtained the molecular envelopes of the Hfq-sRNA complexes using small-angle scattering methods, which reveal key molecular details. These data indicate that Hfq does not substantially change shape upon complex formation, whereas the sRNAs do. We link the impact of Hfq binding, and the sRNA structural changes induced, to transcript stability with respect to RNase E degradation. In light of these findings, we discuss the role of Hfq in the opposing regulatory functions played by RprA and OxyS in rpoS regulation.

Authors+Show Affiliations

Biophysics Laboratories, School of Biological Sciences, Institute of Biomedical and Biomolecular Sciences, University of Portsmouth, Portsmouth, PO1 2DT, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23804244

Citation

Henderson, Charlotte A., et al. "Hfq Binding Changes the Structure of Escherichia Coli Small Noncoding RNAs OxyS and RprA, Which Are Involved in the Riboregulation of RpoS." RNA (New York, N.Y.), vol. 19, no. 8, 2013, pp. 1089-104.
Henderson CA, Vincent HA, Casamento A, et al. Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS. RNA. 2013;19(8):1089-104.
Henderson, C. A., Vincent, H. A., Casamento, A., Stone, C. M., Phillips, J. O., Cary, P. D., Sobott, F., Gowers, D. M., Taylor, J. E., & Callaghan, A. J. (2013). Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS. RNA (New York, N.Y.), 19(8), 1089-104. https://doi.org/10.1261/rna.034595.112
Henderson CA, et al. Hfq Binding Changes the Structure of Escherichia Coli Small Noncoding RNAs OxyS and RprA, Which Are Involved in the Riboregulation of RpoS. RNA. 2013;19(8):1089-104. PubMed PMID: 23804244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS. AU - Henderson,Charlotte A, AU - Vincent,Helen A, AU - Casamento,Alessandra, AU - Stone,Carlanne M, AU - Phillips,Jack O, AU - Cary,Peter D, AU - Sobott,Frank, AU - Gowers,Darren M, AU - Taylor,James E, AU - Callaghan,Anastasia J, Y1 - 2013/06/26/ PY - 2013/6/28/entrez PY - 2013/6/28/pubmed PY - 2013/9/28/medline KW - Hfq KW - OxyS KW - RprA KW - rpoS KW - sRNA SP - 1089 EP - 104 JF - RNA (New York, N.Y.) JO - RNA VL - 19 IS - 8 N2 - OxyS and RprA are two small noncoding RNAs (sRNAs) that modulate the expression of rpoS, encoding an alternative sigma factor that activates transcription of multiple Escherichia coli stress-response genes. While RprA activates rpoS for translation, OxyS down-regulates the transcript. Crucially, the RNA binding protein Hfq is required for both sRNAs to function, although the specific role played by Hfq remains unclear. We have investigated RprA and OxyS interactions with Hfq using biochemical and biophysical approaches. In particular, we have obtained the molecular envelopes of the Hfq-sRNA complexes using small-angle scattering methods, which reveal key molecular details. These data indicate that Hfq does not substantially change shape upon complex formation, whereas the sRNAs do. We link the impact of Hfq binding, and the sRNA structural changes induced, to transcript stability with respect to RNase E degradation. In light of these findings, we discuss the role of Hfq in the opposing regulatory functions played by RprA and OxyS in rpoS regulation. SN - 1469-9001 UR - https://www.unboundmedicine.com/medline/citation/23804244/Hfq_binding_changes_the_structure_of_Escherichia_coli_small_noncoding_RNAs_OxyS_and_RprA_which_are_involved_in_the_riboregulation_of_rpoS_ L2 - http://www.rnajournal.org/cgi/pmidlookup?view=long&pmid=23804244 DB - PRIME DP - Unbound Medicine ER -