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Food-animal related Staphylococcus aureus multidrug-resistant ST9 strains with toxin genes.
Foodborne Pathog Dis. 2013 Sep; 10(9):782-8.FP

Abstract

To determine whether methicillin-susceptible S. aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) are present in commercial pig farms and food products from supermarkets in China, we characterized S. aureus isolates from 250 samples associated with swine and animal-related food products in Shandong Province. The isolates were characterized by susceptibility testing, toxin gene detection, pulse-field gel electrophoresis (PFGE), multilocus sequence typing, and spa typing. MRSA were identified and typed by the staphylococcal cassette chromosome mec (SCCmec). The prevalence of S. aureus among all samples was 19.6% (49/250). MRSA and MSSA accounted for 16.7% (20/120) and 8.3% (10/120), respectively, of swine feces samples. Only MSSA was detected from swine carcass, pork, chicken, and raw milk, accounting for 15% (6/40), 10% (3/30), 20% (6/30), and 13.3% (4/30), respectively. The predominant MRSA clone was ST9-t899 SCCmecIVb/PFGE A (70.0%, 14/20). Among the MSSA isolates, ST9-t899/PFGE A was the most prevalent (27.6%), followed by ST15-t084 (17.2%), ST97-t2756 (10.3%), ST1-t127 (6.9%), and ST398-t899 (3.5%). Some lineages were found that are commonly detected in humans (e.g., ST1, ST5, ST7, ST59, ST88) or are human-specific (e.g., ST15). The toxin genes sec, seh, and enterotoxin gene cluster (egc) were significantly more prevalent among isolates of lineage ST9 (p<0.001) compared to other lineages, and the ST9 isolates were more resistant to erythromycin, clindamycin, ciprofloxacin, chloramphenicol, and gentamicin. The same lineage was identified from different sample types, indicating circulation of the related strains within the area of study. In conclusion, swine and food products of animal origin carried S. aureus, and the predominant ST9 clone possesses a multidrug-resistance profile and a high prevalence of sec, seh, and egc enterotoxin genes.

Authors+Show Affiliations

Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China. whuibj@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23806146

Citation

He, Wenqiang, et al. "Food-animal Related Staphylococcus Aureus Multidrug-resistant ST9 Strains With Toxin Genes." Foodborne Pathogens and Disease, vol. 10, no. 9, 2013, pp. 782-8.
He W, Liu Y, Qi J, et al. Food-animal related Staphylococcus aureus multidrug-resistant ST9 strains with toxin genes. Foodborne Pathog Dis. 2013;10(9):782-8.
He, W., Liu, Y., Qi, J., Chen, H., Zhao, C., Zhang, F., Li, H., & Wang, H. (2013). Food-animal related Staphylococcus aureus multidrug-resistant ST9 strains with toxin genes. Foodborne Pathogens and Disease, 10(9), 782-8. https://doi.org/10.1089/fpd.2012.1452
He W, et al. Food-animal Related Staphylococcus Aureus Multidrug-resistant ST9 Strains With Toxin Genes. Foodborne Pathog Dis. 2013;10(9):782-8. PubMed PMID: 23806146.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Food-animal related Staphylococcus aureus multidrug-resistant ST9 strains with toxin genes. AU - He,Wenqiang, AU - Liu,Yuqing, AU - Qi,Jing, AU - Chen,Hongbin, AU - Zhao,Chunjiang, AU - Zhang,Feifei, AU - Li,Henan, AU - Wang,Hui, Y1 - 2013/06/27/ PY - 2013/6/29/entrez PY - 2013/6/29/pubmed PY - 2014/3/29/medline SP - 782 EP - 8 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 10 IS - 9 N2 - To determine whether methicillin-susceptible S. aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) are present in commercial pig farms and food products from supermarkets in China, we characterized S. aureus isolates from 250 samples associated with swine and animal-related food products in Shandong Province. The isolates were characterized by susceptibility testing, toxin gene detection, pulse-field gel electrophoresis (PFGE), multilocus sequence typing, and spa typing. MRSA were identified and typed by the staphylococcal cassette chromosome mec (SCCmec). The prevalence of S. aureus among all samples was 19.6% (49/250). MRSA and MSSA accounted for 16.7% (20/120) and 8.3% (10/120), respectively, of swine feces samples. Only MSSA was detected from swine carcass, pork, chicken, and raw milk, accounting for 15% (6/40), 10% (3/30), 20% (6/30), and 13.3% (4/30), respectively. The predominant MRSA clone was ST9-t899 SCCmecIVb/PFGE A (70.0%, 14/20). Among the MSSA isolates, ST9-t899/PFGE A was the most prevalent (27.6%), followed by ST15-t084 (17.2%), ST97-t2756 (10.3%), ST1-t127 (6.9%), and ST398-t899 (3.5%). Some lineages were found that are commonly detected in humans (e.g., ST1, ST5, ST7, ST59, ST88) or are human-specific (e.g., ST15). The toxin genes sec, seh, and enterotoxin gene cluster (egc) were significantly more prevalent among isolates of lineage ST9 (p<0.001) compared to other lineages, and the ST9 isolates were more resistant to erythromycin, clindamycin, ciprofloxacin, chloramphenicol, and gentamicin. The same lineage was identified from different sample types, indicating circulation of the related strains within the area of study. In conclusion, swine and food products of animal origin carried S. aureus, and the predominant ST9 clone possesses a multidrug-resistance profile and a high prevalence of sec, seh, and egc enterotoxin genes. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/23806146/Food_animal_related_Staphylococcus_aureus_multidrug_resistant_ST9_strains_with_toxin_genes_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2012.1452?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -