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Role of polyamines in regulating silymarin production in Silybum marianum (L.) Gaertn (Asteraceae) cell cultures under conditions of calcium deficiency.
J Plant Physiol 2013; 170(15):1344-8JP

Abstract

As part of our efforts to identify the possible role of polyamines (PAs) in silymarin (Sm) production, the effects of calcium deprivation on cell growth and on endogenous PAs levels and Sm production by milk thistle (Silybum marianum (L.) Gaertn) grown in cell cultures were examined. Young cultured cells of the H2 line of S. marianum were transferred to a medium without calcium and with ethylene glycol-bis-(β-aminoethyl) ether-N,N,N',N'-tetraacetic acid present to chelate any free calcium in order to analyze the effects of this medium on the levels of PAs and Sm produced by the cells. During the 17 days of exposure to this calcium-free medium most of the cell populations were in the G0/G1 phase (from day 7 to day 14 of culture) while PA levels underwent a progressive decline up to day 17, after which they were no longer detectable. We observed that putrescine (Put) accumulation was always lower than that observed under normal conditions. The lack of calcium in the MS medium advances the onset of the stationary phase, whose beginning is marked by an increase in the Put/spermidine (Spd) index, raising the production of Sm; the suspensions were productive for a longer time and hence produced more of the substance. Our results indicate that under stress conditions the production of Sm in young-cell suspensions of S. marianum is not associated with high levels of PAs in the medium--contrary to what one would expect--allowing us to conclude that growth inhibition appears to be the factor responsible for the maximum Sm accumulation while PAs are not directly involved in the Sm synthesis pathway by milk thistle grown in culture.

Authors+Show Affiliations

Department of Plant Physiology, Faculty of Biology, University of Salamanca, Plaza de los Doctores de la Reina s/n, E-37007 Salamanca, Spain. anaco@usal.esNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23810612

Citation

Cacho, Margarita, et al. "Role of Polyamines in Regulating Silymarin Production in Silybum Marianum (L.) Gaertn (Asteraceae) Cell Cultures Under Conditions of Calcium Deficiency." Journal of Plant Physiology, vol. 170, no. 15, 2013, pp. 1344-8.
Cacho M, Torres Domínguez A, Elena-Rosselló JA. Role of polyamines in regulating silymarin production in Silybum marianum (L.) Gaertn (Asteraceae) cell cultures under conditions of calcium deficiency. J Plant Physiol. 2013;170(15):1344-8.
Cacho, M., Torres Domínguez, A., & Elena-Rosselló, J. A. (2013). Role of polyamines in regulating silymarin production in Silybum marianum (L.) Gaertn (Asteraceae) cell cultures under conditions of calcium deficiency. Journal of Plant Physiology, 170(15), pp. 1344-8. doi:10.1016/j.jplph.2013.05.005.
Cacho M, Torres Domínguez A, Elena-Rosselló JA. Role of Polyamines in Regulating Silymarin Production in Silybum Marianum (L.) Gaertn (Asteraceae) Cell Cultures Under Conditions of Calcium Deficiency. J Plant Physiol. 2013 Oct 15;170(15):1344-8. PubMed PMID: 23810612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of polyamines in regulating silymarin production in Silybum marianum (L.) Gaertn (Asteraceae) cell cultures under conditions of calcium deficiency. AU - Cacho,Margarita, AU - Torres Domínguez,Alexis, AU - Elena-Rosselló,Juana-Ana, Y1 - 2013/06/27/ PY - 2012/11/21/received PY - 2013/05/06/revised PY - 2013/05/07/accepted PY - 2013/7/2/entrez PY - 2013/7/3/pubmed PY - 2014/3/14/medline KW - Ca(2+) deprived KW - EGTA KW - Flavonolignans KW - Growth cycle KW - Milk thistle KW - PAs KW - PH KW - Put KW - Putrescine and plant stress KW - S KW - SH KW - Sm KW - Spd KW - Spm KW - ethylene glycol-bis-(β-aminoethyl) ether-N,N,N′,N′-tetraacetic acid KW - hydrolyzed pellet fraction KW - hydrolyzed soluble fraction KW - polyamines KW - putrescine KW - silymarin KW - soluble fraction KW - spermidine KW - spermine SP - 1344 EP - 8 JF - Journal of plant physiology JO - J. Plant Physiol. VL - 170 IS - 15 N2 - As part of our efforts to identify the possible role of polyamines (PAs) in silymarin (Sm) production, the effects of calcium deprivation on cell growth and on endogenous PAs levels and Sm production by milk thistle (Silybum marianum (L.) Gaertn) grown in cell cultures were examined. Young cultured cells of the H2 line of S. marianum were transferred to a medium without calcium and with ethylene glycol-bis-(β-aminoethyl) ether-N,N,N',N'-tetraacetic acid present to chelate any free calcium in order to analyze the effects of this medium on the levels of PAs and Sm produced by the cells. During the 17 days of exposure to this calcium-free medium most of the cell populations were in the G0/G1 phase (from day 7 to day 14 of culture) while PA levels underwent a progressive decline up to day 17, after which they were no longer detectable. We observed that putrescine (Put) accumulation was always lower than that observed under normal conditions. The lack of calcium in the MS medium advances the onset of the stationary phase, whose beginning is marked by an increase in the Put/spermidine (Spd) index, raising the production of Sm; the suspensions were productive for a longer time and hence produced more of the substance. Our results indicate that under stress conditions the production of Sm in young-cell suspensions of S. marianum is not associated with high levels of PAs in the medium--contrary to what one would expect--allowing us to conclude that growth inhibition appears to be the factor responsible for the maximum Sm accumulation while PAs are not directly involved in the Sm synthesis pathway by milk thistle grown in culture. SN - 1618-1328 UR - https://www.unboundmedicine.com/medline/citation/23810612/Role_of_polyamines_in_regulating_silymarin_production_in_Silybum_marianum__L___Gaertn__Asteraceae__cell_cultures_under_conditions_of_calcium_deficiency_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0176-1617(13)00216-2 DB - PRIME DP - Unbound Medicine ER -