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Effect of blueberry pretreatment on diethylnitrosamine-induced oxidative stress and liver injury in rats.
Environ Toxicol Pharmacol 2013; 36(2):529-538ET

Abstract

Diethylnitrosamine (DEN) treatment increases the generation of reactive oxygen species (ROS), apoptosis, necrosis and proliferation in the liver. Blueberries (BB; Vaccinium corymbosum L.) contain polyphenols and other active components and have high antioxidant capacities. We investigated the effect of BB pretreatment on DEN-induced liver injury and oxidative and nitrosative stress in male rats. Rats were fed with 5% and 10% BB containing diet for six weeks and DEN (200mg/kg; i.p.) was applied two days before the end of this period. Liver function tests were determined in serum and histopathological evaluation was performed in the liver tissue. Apoptosis-related proteins, Bax and B cell lymphoma-2 (Bcl-2) and proliferating cell nuclear antigen (PCNA) expressions were also examined. Oxidative and nitrosative stress were evaluated in the liver by measuring thiobarbituric acid reactive substances, diene conjugate, protein carbonyl and nitrotyrosine levels, and glutathione levels and glutathione peroxidase, superoxide dismutase and glutathione transferase (GST) activities. Pretreatment with high dose of BB reduced apoptotic, necrotic and proliferative changes in the liver induced by DEN. Dietary BB also decreased hepatic lipid peroxidation, protein oxidation and nitrotyrosine levels together with increased GST activity. In conclusion, BB may have an inhibiting effect on acute liver injury by reducing apoptosis, necrosis, proliferation, oxidative and nitrosative stress in DEN-treated rats.

Authors+Show Affiliations

Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.Department of Pathology, Oncology Institute, Istanbul University, Istanbul, Turkey.Department of Pathology, Oncology Institute, Istanbul University, Istanbul, Turkey.Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. Electronic address: sdabbasoglu@yahoo.com.Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23811110

Citation

Bingül, İlknur, et al. "Effect of Blueberry Pretreatment On Diethylnitrosamine-induced Oxidative Stress and Liver Injury in Rats." Environmental Toxicology and Pharmacology, vol. 36, no. 2, 2013, pp. 529-538.
Bingül İ, Başaran-Küçükgergin C, Tekkeşin MS, et al. Effect of blueberry pretreatment on diethylnitrosamine-induced oxidative stress and liver injury in rats. Environ Toxicol Pharmacol. 2013;36(2):529-538.
Bingül, İ., Başaran-Küçükgergin, C., Tekkeşin, M. S., Olgaç, V., Doğru-Abbasoğlu, S., & Uysal, M. (2013). Effect of blueberry pretreatment on diethylnitrosamine-induced oxidative stress and liver injury in rats. Environmental Toxicology and Pharmacology, 36(2), pp. 529-538. doi:10.1016/j.etap.2013.05.014.
Bingül İ, et al. Effect of Blueberry Pretreatment On Diethylnitrosamine-induced Oxidative Stress and Liver Injury in Rats. Environ Toxicol Pharmacol. 2013;36(2):529-538. PubMed PMID: 23811110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of blueberry pretreatment on diethylnitrosamine-induced oxidative stress and liver injury in rats. AU - Bingül,İlknur, AU - Başaran-Küçükgergin,Canan, AU - Tekkeşin,Merva Soluk, AU - Olgaç,Vakur, AU - Doğru-Abbasoğlu,Semra, AU - Uysal,Müjdat, Y1 - 2013/06/10/ PY - 2012/08/16/received PY - 2013/05/29/revised PY - 2013/05/31/accepted PY - 2013/7/2/entrez PY - 2013/7/3/pubmed PY - 2014/3/13/medline KW - Blueberry KW - Diethylnitrosamine KW - Hepatic injury KW - Oxidative stress KW - Rat SP - 529 EP - 538 JF - Environmental toxicology and pharmacology JO - Environ. Toxicol. Pharmacol. VL - 36 IS - 2 N2 - Diethylnitrosamine (DEN) treatment increases the generation of reactive oxygen species (ROS), apoptosis, necrosis and proliferation in the liver. Blueberries (BB; Vaccinium corymbosum L.) contain polyphenols and other active components and have high antioxidant capacities. We investigated the effect of BB pretreatment on DEN-induced liver injury and oxidative and nitrosative stress in male rats. Rats were fed with 5% and 10% BB containing diet for six weeks and DEN (200mg/kg; i.p.) was applied two days before the end of this period. Liver function tests were determined in serum and histopathological evaluation was performed in the liver tissue. Apoptosis-related proteins, Bax and B cell lymphoma-2 (Bcl-2) and proliferating cell nuclear antigen (PCNA) expressions were also examined. Oxidative and nitrosative stress were evaluated in the liver by measuring thiobarbituric acid reactive substances, diene conjugate, protein carbonyl and nitrotyrosine levels, and glutathione levels and glutathione peroxidase, superoxide dismutase and glutathione transferase (GST) activities. Pretreatment with high dose of BB reduced apoptotic, necrotic and proliferative changes in the liver induced by DEN. Dietary BB also decreased hepatic lipid peroxidation, protein oxidation and nitrotyrosine levels together with increased GST activity. In conclusion, BB may have an inhibiting effect on acute liver injury by reducing apoptosis, necrosis, proliferation, oxidative and nitrosative stress in DEN-treated rats. SN - 1872-7077 UR - https://www.unboundmedicine.com/medline/citation/23811110/Effect_of_blueberry_pretreatment_on_diethylnitrosamine_induced_oxidative_stress_and_liver_injury_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1382-6689(13)00127-0 DB - PRIME DP - Unbound Medicine ER -