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An electrochemical immunosensor for sensitive detection of Escherichia coli O157:H7 using C60 based biocompatible platform and enzyme functionalized Pt nanochains tracing tag.
Biosens Bioelectron. 2013 Nov 15; 49:485-91.BB

Abstract

A sensitive and efficient electrochemical immunosensor was designed for amperometric detection of heat-killed Escherichia coli O157:H7 (E. coli O157:H7). The immunosensing platform was first composed of fullerene (C60), ferrocene (Fc) and thiolated chitosan (CHI-SH) composite nano-layer which could offer rich -SH functional groups and maintain good biocompatibility. Then the Au nanoparticles coated SiO2 nanocomposites (Au-SiO2) were assembled on the CHI-SH/Fc/C60 composite. Next, the large amount of avidin (SA) was coated on the Au-SiO2 surface, which was used to immobilize biotinylated capture antibodies of E. coli O157:H7 (bio-Ab1) through the covalent reaction between biotin and avidin. With surface area enhancement by C60 and Au-SiO2, and directional immobilization by avidin-biotin system, the amount of immobilized bio-Ab1 can be enhanced obviously. For signal amplification, the glucose oxidase (GOD) loaded Pt nanochains (PtNCs) were used as tracing tag to label signal antibodies (Ab2). With a sandwich-type immunoreaction, the concentration volume of heat-killed E. coli O157:H7 ranged from 3.2 × 10(1) to 3.2 × 10(6)CFU/mL with a limit of detection down to 15 CFU/mL (S/N=3), which could be well accepted for early clinical detection. The studied system provides new opportunities, and might speed up disease diagnosis, treatment and prevention with pathogen.

Authors+Show Affiliations

Department of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23811483

Citation

Li, Yan, et al. "An Electrochemical Immunosensor for Sensitive Detection of Escherichia Coli O157:H7 Using C60 Based Biocompatible Platform and Enzyme Functionalized Pt Nanochains Tracing Tag." Biosensors & Bioelectronics, vol. 49, 2013, pp. 485-91.
Li Y, Fang L, Cheng P, et al. An electrochemical immunosensor for sensitive detection of Escherichia coli O157:H7 using C60 based biocompatible platform and enzyme functionalized Pt nanochains tracing tag. Biosens Bioelectron. 2013;49:485-91.
Li, Y., Fang, L., Cheng, P., Deng, J., Jiang, L., Huang, H., & Zheng, J. (2013). An electrochemical immunosensor for sensitive detection of Escherichia coli O157:H7 using C60 based biocompatible platform and enzyme functionalized Pt nanochains tracing tag. Biosensors & Bioelectronics, 49, 485-91. https://doi.org/10.1016/j.bios.2013.06.008
Li Y, et al. An Electrochemical Immunosensor for Sensitive Detection of Escherichia Coli O157:H7 Using C60 Based Biocompatible Platform and Enzyme Functionalized Pt Nanochains Tracing Tag. Biosens Bioelectron. 2013 Nov 15;49:485-91. PubMed PMID: 23811483.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An electrochemical immunosensor for sensitive detection of Escherichia coli O157:H7 using C60 based biocompatible platform and enzyme functionalized Pt nanochains tracing tag. AU - Li,Yan, AU - Fang,Lichao, AU - Cheng,Ping, AU - Deng,Jun, AU - Jiang,Lili, AU - Huang,Hui, AU - Zheng,Junsong, Y1 - 2013/06/13/ PY - 2013/04/23/received PY - 2013/06/04/revised PY - 2013/06/05/accepted PY - 2013/7/2/entrez PY - 2013/7/3/pubmed PY - 2014/2/14/medline KW - Au nanoparticles coated SiO(2) nanocomposites KW - Escherichia coli O157:H7 KW - Fullerene KW - Immunosensor KW - Pt nanochains SP - 485 EP - 91 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 49 N2 - A sensitive and efficient electrochemical immunosensor was designed for amperometric detection of heat-killed Escherichia coli O157:H7 (E. coli O157:H7). The immunosensing platform was first composed of fullerene (C60), ferrocene (Fc) and thiolated chitosan (CHI-SH) composite nano-layer which could offer rich -SH functional groups and maintain good biocompatibility. Then the Au nanoparticles coated SiO2 nanocomposites (Au-SiO2) were assembled on the CHI-SH/Fc/C60 composite. Next, the large amount of avidin (SA) was coated on the Au-SiO2 surface, which was used to immobilize biotinylated capture antibodies of E. coli O157:H7 (bio-Ab1) through the covalent reaction between biotin and avidin. With surface area enhancement by C60 and Au-SiO2, and directional immobilization by avidin-biotin system, the amount of immobilized bio-Ab1 can be enhanced obviously. For signal amplification, the glucose oxidase (GOD) loaded Pt nanochains (PtNCs) were used as tracing tag to label signal antibodies (Ab2). With a sandwich-type immunoreaction, the concentration volume of heat-killed E. coli O157:H7 ranged from 3.2 × 10(1) to 3.2 × 10(6)CFU/mL with a limit of detection down to 15 CFU/mL (S/N=3), which could be well accepted for early clinical detection. The studied system provides new opportunities, and might speed up disease diagnosis, treatment and prevention with pathogen. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23811483/An_electrochemical_immunosensor_for_sensitive_detection_of_Escherichia_coli_O157:H7_using_C60_based_biocompatible_platform_and_enzyme_functionalized_Pt_nanochains_tracing_tag_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00405-3 DB - PRIME DP - Unbound Medicine ER -