TY - JOUR T1 - An electrochemical immunosensor for sensitive detection of Escherichia coli O157:H7 using C60 based biocompatible platform and enzyme functionalized Pt nanochains tracing tag. AU - Li,Yan, AU - Fang,Lichao, AU - Cheng,Ping, AU - Deng,Jun, AU - Jiang,Lili, AU - Huang,Hui, AU - Zheng,Junsong, Y1 - 2013/06/13/ PY - 2013/04/23/received PY - 2013/06/04/revised PY - 2013/06/05/accepted PY - 2013/7/2/entrez PY - 2013/7/3/pubmed PY - 2014/2/14/medline KW - Au nanoparticles coated SiO(2) nanocomposites KW - Escherichia coli O157:H7 KW - Fullerene KW - Immunosensor KW - Pt nanochains SP - 485 EP - 91 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 49 N2 - A sensitive and efficient electrochemical immunosensor was designed for amperometric detection of heat-killed Escherichia coli O157:H7 (E. coli O157:H7). The immunosensing platform was first composed of fullerene (C60), ferrocene (Fc) and thiolated chitosan (CHI-SH) composite nano-layer which could offer rich -SH functional groups and maintain good biocompatibility. Then the Au nanoparticles coated SiO2 nanocomposites (Au-SiO2) were assembled on the CHI-SH/Fc/C60 composite. Next, the large amount of avidin (SA) was coated on the Au-SiO2 surface, which was used to immobilize biotinylated capture antibodies of E. coli O157:H7 (bio-Ab1) through the covalent reaction between biotin and avidin. With surface area enhancement by C60 and Au-SiO2, and directional immobilization by avidin-biotin system, the amount of immobilized bio-Ab1 can be enhanced obviously. For signal amplification, the glucose oxidase (GOD) loaded Pt nanochains (PtNCs) were used as tracing tag to label signal antibodies (Ab2). With a sandwich-type immunoreaction, the concentration volume of heat-killed E. coli O157:H7 ranged from 3.2 × 10(1) to 3.2 × 10(6)CFU/mL with a limit of detection down to 15 CFU/mL (S/N=3), which could be well accepted for early clinical detection. The studied system provides new opportunities, and might speed up disease diagnosis, treatment and prevention with pathogen. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23811483/An_electrochemical_immunosensor_for_sensitive_detection_of_Escherichia_coli_O157:H7_using_C60_based_biocompatible_platform_and_enzyme_functionalized_Pt_nanochains_tracing_tag_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00405-3 DB - PRIME DP - Unbound Medicine ER -