Tags

Type your tag names separated by a space and hit enter

Impedimetric immunosensor based on gold nanoparticles modified graphene paper for label-free detection of Escherichia coli O157:H7.
Biosens Bioelectron. 2013 Nov 15; 49:492-8.BB

Abstract

In this study, a low-cost and robust impedimetric immunosensor based on gold nanoparticles modified free-standing graphene paper electrode for rapid and sensitive detection of Escherichia coli O157:H7 (E. coli O157:H7) was developed. Graphene paper was prepared by chemical reduction of graphene oxide paper obtained from vacuum filtration method. Scanning electron microscope, Raman spectroscopy and X-ray diffraction techniques were employed to investigate the surface morphology and crystal structure of the prepared graphene paper. The gold nanoparticles were grown on the surface of graphene paper electrode by one-step electrodeposition technique. The immobilization of anti-E. coli O157:H7 antibodies on paper electrode were performed via biotin-streptavidin system. Electrochemical impedance spectroscopy was used to detect E. coli O157:H7 captured on the paper electrode. Results show that the developed paper immunosensor possesses greatly enhanced sensing performance, such as wide linear range (1.5 × 10(2)-1.5 × 10(7) cfu mL(-1)), low detection limit (1.5 × 10(2) cfu mL(-1)), and excellent specificity. Furthermore, flexible test demonstrate the graphene paper based sensing device has high tolerability to mechanical stress. The strategy of structurally integrating metal nanomaterials, graphene paper, and biorecognition molecules would provide new insight into design of flexible immunosensors for routine sensing applications.

Authors+Show Affiliations

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23811484

Citation

Wang, Yixian, et al. "Impedimetric Immunosensor Based On Gold Nanoparticles Modified Graphene Paper for Label-free Detection of Escherichia Coli O157:H7." Biosensors & Bioelectronics, vol. 49, 2013, pp. 492-8.
Wang Y, Ping J, Ye Z, et al. Impedimetric immunosensor based on gold nanoparticles modified graphene paper for label-free detection of Escherichia coli O157:H7. Biosens Bioelectron. 2013;49:492-8.
Wang, Y., Ping, J., Ye, Z., Wu, J., & Ying, Y. (2013). Impedimetric immunosensor based on gold nanoparticles modified graphene paper for label-free detection of Escherichia coli O157:H7. Biosensors & Bioelectronics, 49, 492-8. https://doi.org/10.1016/j.bios.2013.05.061
Wang Y, et al. Impedimetric Immunosensor Based On Gold Nanoparticles Modified Graphene Paper for Label-free Detection of Escherichia Coli O157:H7. Biosens Bioelectron. 2013 Nov 15;49:492-8. PubMed PMID: 23811484.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impedimetric immunosensor based on gold nanoparticles modified graphene paper for label-free detection of Escherichia coli O157:H7. AU - Wang,Yixian, AU - Ping,Jianfeng, AU - Ye,Zunzhong, AU - Wu,Jian, AU - Ying,Yibin, Y1 - 2013/06/11/ PY - 2013/04/20/received PY - 2013/05/27/revised PY - 2013/05/31/accepted PY - 2013/7/2/entrez PY - 2013/7/3/pubmed PY - 2014/2/14/medline KW - Gold nanoparticles KW - Graphene paper KW - Impedimetric immunosensor KW - Lab-free detection SP - 492 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 49 N2 - In this study, a low-cost and robust impedimetric immunosensor based on gold nanoparticles modified free-standing graphene paper electrode for rapid and sensitive detection of Escherichia coli O157:H7 (E. coli O157:H7) was developed. Graphene paper was prepared by chemical reduction of graphene oxide paper obtained from vacuum filtration method. Scanning electron microscope, Raman spectroscopy and X-ray diffraction techniques were employed to investigate the surface morphology and crystal structure of the prepared graphene paper. The gold nanoparticles were grown on the surface of graphene paper electrode by one-step electrodeposition technique. The immobilization of anti-E. coli O157:H7 antibodies on paper electrode were performed via biotin-streptavidin system. Electrochemical impedance spectroscopy was used to detect E. coli O157:H7 captured on the paper electrode. Results show that the developed paper immunosensor possesses greatly enhanced sensing performance, such as wide linear range (1.5 × 10(2)-1.5 × 10(7) cfu mL(-1)), low detection limit (1.5 × 10(2) cfu mL(-1)), and excellent specificity. Furthermore, flexible test demonstrate the graphene paper based sensing device has high tolerability to mechanical stress. The strategy of structurally integrating metal nanomaterials, graphene paper, and biorecognition molecules would provide new insight into design of flexible immunosensors for routine sensing applications. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23811484/Impedimetric_immunosensor_based_on_gold_nanoparticles_modified_graphene_paper_for_label_free_detection_of_Escherichia_coli_O157:H7_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00395-3 DB - PRIME DP - Unbound Medicine ER -