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Remote ischaemic preconditioning down-regulates kinin receptor expression in neutrophils of patients undergoing heart surgery.
Interact Cardiovasc Thorac Surg. 2013 Oct; 17(4):653-8.IC

Abstract

OBJECTIVES

Remote ischaemic preconditioning (RIPC) may protect distant organs against ischaemia-reperfusion injury. We investigated the impact of RIPC on kinin receptor expression in neutrophils following RIPC in patients undergoing coronary artery bypass grafting (CABG).

METHODS

Patients undergoing elective CABG with cardiopulmonary bypass (CPB) were randomized to RIPC (n = 15) or control (n = 15) groups. The study group underwent RIPC by inflation of a blood pressure cuff on the arm. Expression of kinin receptors, plasma concentrations of IL-6, IL-8, IL-10, TNF-α and neutrophil elastase were determined at baseline (before RIPC/sham), immediately before surgery (after RIPC/sham) and 30 min and 24 h after surgery. Plasma bradykinin levels were assessed before and after RIPC/sham, and at 30 min, 6, 12 and 24 h after surgery. Serum creatine kinase (CK), troponin I, C-reactive protein (CRP) and lactate levels were measured immediately prior to surgery and 30 min, 6, 12, 24 and 48 h after surgery.

RESULTS

Kinin B2 receptor expression did not differ between the groups at baseline (pre-RIPC), but was significantly lower in the RIPC group than in the control group after RIPC/sham (P < 0.05). Expressions of both kinin B1 and B2 receptors were significantly down-regulated in the RIPC group, and this persisted to 24 h after surgery (P < 0.001). Neutrophil elastase levels were significantly increased after surgery. There were no differences in CK, CRP, cytokine, lactate or troponin I levels between the groups.

CONCLUSIONS

RIPC down-regulated the expression of kinin B1 and B2 receptors in neutrophils of patients undergoing CABG.

Authors+Show Affiliations

School of Surgery, University of Western Australia, Perth, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23814135

Citation

Saxena, Pankaj, et al. "Remote Ischaemic Preconditioning Down-regulates Kinin Receptor Expression in Neutrophils of Patients Undergoing Heart Surgery." Interactive Cardiovascular and Thoracic Surgery, vol. 17, no. 4, 2013, pp. 653-8.
Saxena P, Aggarwal S, Misso NL, et al. Remote ischaemic preconditioning down-regulates kinin receptor expression in neutrophils of patients undergoing heart surgery. Interact Cardiovasc Thorac Surg. 2013;17(4):653-8.
Saxena, P., Aggarwal, S., Misso, N. L., Passage, J., Newman, M. A., Thompson, P. J., d'Udekem, Y., Praporski, S., & Konstantinov, I. E. (2013). Remote ischaemic preconditioning down-regulates kinin receptor expression in neutrophils of patients undergoing heart surgery. Interactive Cardiovascular and Thoracic Surgery, 17(4), 653-8. https://doi.org/10.1093/icvts/ivt279
Saxena P, et al. Remote Ischaemic Preconditioning Down-regulates Kinin Receptor Expression in Neutrophils of Patients Undergoing Heart Surgery. Interact Cardiovasc Thorac Surg. 2013;17(4):653-8. PubMed PMID: 23814135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Remote ischaemic preconditioning down-regulates kinin receptor expression in neutrophils of patients undergoing heart surgery. AU - Saxena,Pankaj, AU - Aggarwal,Shashi, AU - Misso,Neil L, AU - Passage,Jurgen, AU - Newman,Mark A J, AU - Thompson,Philip J, AU - d'Udekem,Yves, AU - Praporski,Slavica, AU - Konstantinov,Igor E, Y1 - 2013/06/27/ PY - 2013/7/2/entrez PY - 2013/7/3/pubmed PY - 2014/5/3/medline KW - Bradykinin KW - Cardiac surgery KW - Ischaemia KW - Ischaemic preconditioning KW - Kinin receptors SP - 653 EP - 8 JF - Interactive cardiovascular and thoracic surgery JO - Interact Cardiovasc Thorac Surg VL - 17 IS - 4 N2 - OBJECTIVES: Remote ischaemic preconditioning (RIPC) may protect distant organs against ischaemia-reperfusion injury. We investigated the impact of RIPC on kinin receptor expression in neutrophils following RIPC in patients undergoing coronary artery bypass grafting (CABG). METHODS: Patients undergoing elective CABG with cardiopulmonary bypass (CPB) were randomized to RIPC (n = 15) or control (n = 15) groups. The study group underwent RIPC by inflation of a blood pressure cuff on the arm. Expression of kinin receptors, plasma concentrations of IL-6, IL-8, IL-10, TNF-α and neutrophil elastase were determined at baseline (before RIPC/sham), immediately before surgery (after RIPC/sham) and 30 min and 24 h after surgery. Plasma bradykinin levels were assessed before and after RIPC/sham, and at 30 min, 6, 12 and 24 h after surgery. Serum creatine kinase (CK), troponin I, C-reactive protein (CRP) and lactate levels were measured immediately prior to surgery and 30 min, 6, 12, 24 and 48 h after surgery. RESULTS: Kinin B2 receptor expression did not differ between the groups at baseline (pre-RIPC), but was significantly lower in the RIPC group than in the control group after RIPC/sham (P < 0.05). Expressions of both kinin B1 and B2 receptors were significantly down-regulated in the RIPC group, and this persisted to 24 h after surgery (P < 0.001). Neutrophil elastase levels were significantly increased after surgery. There were no differences in CK, CRP, cytokine, lactate or troponin I levels between the groups. CONCLUSIONS: RIPC down-regulated the expression of kinin B1 and B2 receptors in neutrophils of patients undergoing CABG. SN - 1569-9285 UR - https://www.unboundmedicine.com/medline/citation/23814135/Remote_ischaemic_preconditioning_down_regulates_kinin_receptor_expression_in_neutrophils_of_patients_undergoing_heart_surgery_ L2 - https://academic.oup.com/icvts/article-lookup/doi/10.1093/icvts/ivt279 DB - PRIME DP - Unbound Medicine ER -