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A novel aptasensor for the ultra-sensitive detection of adenosine triphosphate via aptamer/quantum dot based resonance energy transfer.
Analyst. 2013 Sep 07; 138(17):4732-6.A

Abstract

We designed a novel aptamer based biosensor (aptasensor) for ultrasensitive detection of adenosine triphosphate (ATP) through resonance energy transfer (RET). The ATP aptamer was modified with Cy3 at the 3' end, and a green quantum dot (525) was attached to the 5' end of its complementary sequence respectively. The ATP aptamer and its complementary sequence could assemble into a duplex structure in the absence of target ATP, and then decrease the distance between the quantum dot and Cy3 which could produce significant RET signal. Upon ATP binding, the ATP aptamer could dissociate with its complementary sequence and then increase the distance between the quantum dot and Cy3 which would significantly decrease the RET signal. Therefore, the ATP detection could be easily achieved through detection of the fluorescence intensity ratio between 525 nm and 560 nm. The results show that the emission fluorescence intensity ratio of 525/560 is linearly related to the logarithmic concentration of ATP. The linear range of this aptasensor is from 0.1 nM to 1 μM, and the detection limit is lower down to 0.01 nM. Excellent selectivity of this aptasensor for ATP has been demonstrated through the detection of thymidine triphosphate (TTP), cytidine triphosphate (CTP), guanosine triphosphate (GTP) and adenosine diphosphate (ADP) respectively as control. The method we described here could easily detect ATP with excellent selectivity, linearity and sensitivity down to the nanomolar range, as well as avoid photobleaching.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li Z
Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, People's Republic of China.
Wang Y
No affiliation info available
Liu Y
No affiliation info available
Zeng Y
No affiliation info available
Huang A
No affiliation info available
Peng N
No affiliation info available
Liu X
No affiliation info available
Liu J
No affiliation info available

MeSH

Adenosine TriphosphateAptamers, NucleotideBase SequenceBiosensing TechniquesFluorescence Resonance Energy TransferQuantum Dots

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23814782

Citation

Li, Zheng, et al. "A Novel Aptasensor for the Ultra-sensitive Detection of Adenosine Triphosphate Via Aptamer/quantum Dot Based Resonance Energy Transfer." The Analyst, vol. 138, no. 17, 2013, pp. 4732-6.
Li Z, Wang Y, Liu Y, et al. A novel aptasensor for the ultra-sensitive detection of adenosine triphosphate via aptamer/quantum dot based resonance energy transfer. Analyst. 2013;138(17):4732-6.
Li, Z., Wang, Y., Liu, Y., Zeng, Y., Huang, A., Peng, N., Liu, X., & Liu, J. (2013). A novel aptasensor for the ultra-sensitive detection of adenosine triphosphate via aptamer/quantum dot based resonance energy transfer. The Analyst, 138(17), 4732-6. https://doi.org/10.1039/c3an00449j
Li Z, et al. A Novel Aptasensor for the Ultra-sensitive Detection of Adenosine Triphosphate Via Aptamer/quantum Dot Based Resonance Energy Transfer. Analyst. 2013 Sep 7;138(17):4732-6. PubMed PMID: 23814782.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel aptasensor for the ultra-sensitive detection of adenosine triphosphate via aptamer/quantum dot based resonance energy transfer. AU - Li,Zheng, AU - Wang,Yijing, AU - Liu,Ying, AU - Zeng,Yongyi, AU - Huang,Aimin, AU - Peng,Niancai, AU - Liu,Xiaolong, AU - Liu,Jingfeng, Y1 - 2013/07/01/ PY - 2013/7/2/entrez PY - 2013/7/3/pubmed PY - 2014/1/29/medline SP - 4732 EP - 6 JF - The Analyst JO - Analyst VL - 138 IS - 17 N2 - We designed a novel aptamer based biosensor (aptasensor) for ultrasensitive detection of adenosine triphosphate (ATP) through resonance energy transfer (RET). The ATP aptamer was modified with Cy3 at the 3' end, and a green quantum dot (525) was attached to the 5' end of its complementary sequence respectively. The ATP aptamer and its complementary sequence could assemble into a duplex structure in the absence of target ATP, and then decrease the distance between the quantum dot and Cy3 which could produce significant RET signal. Upon ATP binding, the ATP aptamer could dissociate with its complementary sequence and then increase the distance between the quantum dot and Cy3 which would significantly decrease the RET signal. Therefore, the ATP detection could be easily achieved through detection of the fluorescence intensity ratio between 525 nm and 560 nm. The results show that the emission fluorescence intensity ratio of 525/560 is linearly related to the logarithmic concentration of ATP. The linear range of this aptasensor is from 0.1 nM to 1 μM, and the detection limit is lower down to 0.01 nM. Excellent selectivity of this aptasensor for ATP has been demonstrated through the detection of thymidine triphosphate (TTP), cytidine triphosphate (CTP), guanosine triphosphate (GTP) and adenosine diphosphate (ADP) respectively as control. The method we described here could easily detect ATP with excellent selectivity, linearity and sensitivity down to the nanomolar range, as well as avoid photobleaching. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/23814782/A_novel_aptasensor_for_the_ultra_sensitive_detection_of_adenosine_triphosphate_via_aptamer/quantum_dot_based_resonance_energy_transfer_ L2 - https://doi.org/10.1039/c3an00449j DB - PRIME DP - Unbound Medicine ER -